LOCUS       sequence-472653-        6023 bp    DNA     circular SYN 14-MAY-2025
DEFINITION  IVT mRNA of human gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Soto DC, Uribe-Salazar JM, Kaya G, Valdarrago R, Sekar A, Haghani 
            NK, Hino K, La GN, Mariano NAF, Ingamells C, Baraban AE, Turner TN, 
            Green ED, Simo S, Quon G, Andres AM, Dennis MY
  TITLE     Gene expansions contributing to human brain evolution.
  JOURNAL   bioRxiv [Preprint]. 2024 Sep 26:2024.09.26.615256. doi: 
            10.1101/2024.09.26.615256.
  PUBMED    39386494
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Sep 26:2024.09.26.615256. doi: 
            10.1101/2024.09.26.615256."
COMMENT     Sequence Label: GPR89B_full_pGCS1
FEATURES             Location/Qualifiers
     source          1..6023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    1143..1277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1180..1199)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1234..1253
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    1307..1331
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3235..3259)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3299..3317)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3300..3317)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(3352..4336)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     rep_origin      4407..4862
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4494..4513)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4704..4725
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4888..4992
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4993..5061,5062..5853)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5211..5230)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgctccc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tgggtaccgg gcccaatgca ttggcgccgc ggccgcgaat
     1081 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     1141 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     1201 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     1261 cttatcatgt ctggatctac gtaatacgac tcactatagt tctagaacca ctttgtacaa
     1321 gaaagctggg ttaggaggtg tgggatgcta actcatgtta gatcacacaa gcataataaa
     1381 taccaagcta tgcttgataa aatgaacgta aggcaaaagt gttacagtct ctgattttaa
     1441 tgagtcaatc tcaaggcaaa gctataacct tttccatgtg aaccttaaaa cggaaatcct
     1501 acgtgtttgg ctcagctacc atagacatct cttgccccag agttccagtg tatttttcac
     1561 ctttagtttc ttggctcctc tccgcctcta caccagcctc atatccacac gggatgctct
     1621 ctgctgagta ttgtcttgag ttagttctcc ctctcatgtt ctggctgatg ctactcatgg
     1681 tcatagtatc acctgagggg gaaggagtat gctatgaagg tgaaaaatgc taccatagca
     1741 ttttgtttgt ttataaaatg tcaggccctg gttccatttt tcccccctct tatatctaaa
     1801 ttttgaaacc actggcctct aacagtctgt agtaggctta agttcaaggt gccatttgct
     1861 tctctggtgc ctgtttgtga gccaaataga ggaagagtat gctagagaga gcgctgacca
     1921 ggaagatcac atcaaaccaa cggtgataga agttgaactg cagttctcca aggacttcag
     1981 tgattatggt gcggtattct aaaggcatac tcattcggat cagcagcaca gaggagacaa
     2041 agtacatgcc cattatctgt gctaatagca ggacaatgac attggaggac ttactgctag
     2101 agatggcata aaagaacttg gtaagagtga tcagcaatcc tctgatggat gtgacgatga
     2161 ttattccaac aagaatgaag gaaatgtgtt gggaccaaaa cttcacatca aattggattc
     2221 ccagataatt cacagtgatc tcaatgcctc ttgtgacagg atccgttttc ccaactcgat
     2281 caaaaacaat attgatggta gccatgaaaa ttttccaaac acagtaaata gagaaaaagt
     2341 aaccaagaaa attaaaatat ttccccttga aggttttgga gtattctatt ctctccttgg
     2401 tagcatatag atcagctgtt tccagaaaaa gctgcctgct taattcttcc aaagcatcca
     2461 cttcctgttg aataagagta agattttcac ttcctgatgc tgaagtggta acacttttta
     2521 tcattcccca gaaacctgat ggtttgttat gcacttcccc cttctggaac attgttctcc
     2581 gtgccattgc catccttttc tttttgctta tgatcatatc catggtttgc agcagtcgcc
     2641 gttccagggc tagaatatcc gtgtcagtca cattcctgag gaagtaagac atgtaagtgt
     2701 atgggcagtt gacagcacca aatccagaaa gaagagccat gagagtcact ccaatcacac
     2761 caacccggct gatgagctgt tctatggata agatcccatg ttttgggctg agaatgggaa
     2821 agggatctcc tagtttccag aagaaataca taaaggtcag ccataagaga caggaaaaaa
     2881 gcagtcgttg tttatgcagt agtcggatat tgctcacaat aaaatagcca atgtaaaaag
     2941 gcaccatgaa aaccaggatc agcagaatta cacacaggtt cattttccag tgaaaataac
     3001 gggagctgct attcaatact cctaagattt caaagatgat gagctcaaac atggtgcaag
     3061 aaaatgcaaa cgtcacggag aagatcacct gtacaacata ctgacgtatc tcatagtctt
     3121 taaacaattg gcgcatgaag aaaagccacc caaatccaaa aaatagtatc tgggaggtaa
     3181 tcatgatgct ggagtcgatc aggaaactca tggcgaagtg taaggaaggc tcgaagcctg
     3241 cttttttgta caaacttgtg gccggccgga tcctgcaaaa agaacaagta gcttgtattc
     3301 tatagtgtca cctaaatcaa gcttgctccg acgtccccag gcagaatggc ggttccctaa
     3361 acgagcattg cttatataga cctcccatta ggcacgccta ccgcccattt acgtcaatgg
     3421 aacgcccatt tgcgtcattg cccctcccca ttgacgtcaa tggggatgta cttggcagcc
     3481 atcgcgggcc atttaccgcc attgacgtca atgggagtac tgccaatgta ccctggcgta
     3541 cttccaatag taatgtactt gccaagttac tattaataga tattgatgta ctgccaagtg
     3601 ggccatttac cgtcattgac gtcaataggg ggcgtgagaa cggatatgaa tgggcaatga
     3661 gccatcccat tgacgtcaat ggtgggtggt cctattgacg tcaatgggca ttgagccagg
     3721 cgggccattt accgtaattg acgtcaatgg gggaggcgcc atatacgtca ataggaccgc
     3781 ccatatgacg tcaataggaa agaccatata tagagaccat tgacgtcaat gggggagtgg
     3841 ctatgggcgg tattaggaag ccccatatat ggtatatggg accgcccatt gggaggggct
     3901 atctacgtca ataggaaaac ccatatatgg aatactatat ggcatagggc caatacatag
     3961 tattgaacct ggccaatagc catattggca tagggccata ttggatattg cctatatatt
     4021 gatcctggca tatagccaat atggccgcca ttattggcac catgccaatt caatatggcg
     4081 gacttggcac agggccaaga cccctcctca aacttggcac cgtgccaagt agacccctcc
     4141 ccagttggca cagtgccagg tccgccatat tgaattggca tggtgccaag tccgccatat
     4201 tgaattggct ggggtccaga tccaccatat tgaattggca cgagtccata tacgccatat
     4261 tgaattggca caggtccaga tccaccatat tgaattggca tgagtccata tacgccatat
     4321 tgaattggcg gtcgactggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4381 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     4441 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     4501 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4561 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4621 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4681 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     4741 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     4801 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     4861 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4921 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4981 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     5041 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5101 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5161 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5221 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5281 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5341 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5401 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5461 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5521 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5581 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5641 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5701 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5761 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5821 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5881 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5941 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     6001 gtagaaaaga tcaaaggatc ttc
//
