LOCUS       sequence-47267-e         838 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3110-01
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(45..725)
                     /codon_start=1
                     /product="monomeric variant of green-to-red photoswitchable
                     fluorescent protein EosFP (McKinney et al., 2009)"
                     /label=mEos2
                     /translation="MSAIKPDMKIKLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAKYPDNIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFYGTNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIHMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     promoter        complement(810..828)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(811..828)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 aatcataaat cataagaaat tcgcttattt agaagtggcg cgccctatcg tctggcattg
       61 tcaggcaatc cagaatgagc aacagcatgc tcatacagct taaccttgtt gtaatctttg
      121 tcatggctta aaatctcaat gcagtggtcc acaaagtggt agcctggtaa cttgacaccc
      181 ttctccttag ctttgtaagt agttctgaag tcacatcggt aatgggcatt tccttcaagc
      241 aacaaagcca tatgaatatc acccgtcagc actccatcac gcacatacat tttctcagtg
      301 gagggctccc atttcagcgt cttcttctgc ataactggac cattggcggg aaagttggta
      361 ccataaaatc gaactttatt atagaaagtg tccccttcca ttgttatgtc gtttctggca
      421 atgcaaatgc ccccgtcttc gaaagtcaag cttcgttccc acgaataccc cttaggaaac
      481 gactgcttaa aatagtcttg tatgttgtct ggatatttgg cgaataccct gttgccgtaa
      541 tggaatgcag tggtcaggat atcaaaggca aaaggcagag gtccgccctc tttgacttca
      601 agatccatac tctgttttcc ctcaaaaggc ttgcctgtac catctccgtc gatcacaaag
      661 tggtgcccgt ttacgttgcc ttccatacgg agtttgatct tcatgtctgg cttaatcgca
      721 ctcatgttaa ttaaaccagc accgtcaccg atcttaagcc cggggatccg tcgacctgca
      781 gcgtacgaag cttcagctgg cggccgcgtt ctatagtgtc acctaaatcg tatgtgta
//
