LOCUS       sequence-472725-        2638 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous CCP promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdCCP
FEATURES             Location/Qualifiers
     source          1..2638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     290..309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(528..632)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     700..718
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(756..778)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     878..897
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1546..2134)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1626..1645)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2305..2638,1..458,459..527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
       61 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
      121 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
      181 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
      241 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
      301 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      361 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
      421 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      481 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
      541 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
      601 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
      661 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
      721 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
      781 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
      841 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
      901 ggtctcagtg cagtgtacag aacatacaaa cgcgtgtgcg tcgggcttgg atgagtctgt
      961 agtcttttga tgaacgatga aggaggtcta tgtcgtattc agatctgttg ttgtccaaag
     1021 cttcatgtga gaaatcttat aggatctgtc cgtgttcgac ggggtcgcta ctcagacaaa
     1081 ccagagtgtc ctattgtaaa tgctacggaa ataataacaa atacagaggg ttgatggtca
     1141 agaacatcgt ccattgtggg ggacacgaca ttcccggtgt ggggggtttt aaactattgt
     1201 cgtcatatgt atgtagaata agagtaaccc taactttccc aatgtggaaa aacttgcagt
     1261 cagatgattg atccttccgg tcatgccgga gttctccagc cttaaaggca gcggggtgag
     1321 taagtaaatg tatcaactca cttccttctt atccatccat ctaaacctac cttacctttt
     1381 gtgtccgcga aaataatcag taataatgag agaccgagcg gtatcagctc actcaaaggc
     1441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1741 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagcc
     1861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1981 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     2041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     2101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     2161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     2221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2341 gatctgtcta tttcgttcat ccatagttgc ctggctcccc gtcgtgtaga taactacgat
     2401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc cacgctcacc
     2461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtca
//
