LOCUS       sequence-472727-        2711 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous Nth promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdNth
FEATURES             Location/Qualifiers
     source          1..2711
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     467..486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(705..809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     877..895
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(933..955)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1055..1074
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1796..2384)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1876..1895)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2555..2711,1..635,636..704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
       61 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      121 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      181 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
      241 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      301 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      361 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      421 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      481 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      541 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      601 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      661 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      721 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      781 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      841 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      901 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
      961 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     1021 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgggt
     1081 ctcagtgcat gttctattgt tctctgtcat tacctgtggc tagctatatg tactaccccg
     1141 agaactactc ttcttgattt catctatgat cgtactccct gttgtgcctc gatgtatatt
     1201 atgagagaat ttctggctgt taatatacgt tgcttgatat ctggaacgat tctcgttgat
     1261 ctggtgtcgt tctcctccac gtatctaata tggtcgacat caaagatctt gtgagcgaac
     1321 tgtattttta acatgagtgt tgttcatgtt aatttctttg atagttagcc caataaatcc
     1381 tctccttctc tcgtcatcat caagagtcag atagaactga aagtgtcgaa accctattca
     1441 aaactcgcga taagaaactc ttctgaacat agatcaagaa ggaatatctc agatggtcaa
     1501 gcctaaccaa acctatcaat gaattcaaga gtttgctaca tcttatctta tctctatcac
     1561 ggcgatcggg aaaatgaaca atgacgcaaa tctaggctta aaatctcgat cccttgagct
     1621 cgtccgggta cccctcttct tactttgtac atccaatgag agaccgagcg gtatcagctc
     1681 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1741 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     1801 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1861 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1921 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1981 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2041 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2101 gtcttgagcc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2161 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2221 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2281 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2341 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2401 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2461 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2521 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2581 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctggctcccc gtcgtgtaga
     2641 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc
     2701 cacgctcacc g
//
