LOCUS       sequence-472730-        2662 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous MSS4 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdMSS4
FEATURES             Location/Qualifiers
     source          1..2662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     467..486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(705..809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     877..895
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(933..955)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1055..1074
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1747..2335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1827..1846)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2506..2662,1..635,636..704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
       61 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      121 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      181 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
      241 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      301 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      361 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      421 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      481 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      541 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      601 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      661 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      721 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      781 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      841 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      901 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
      961 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     1021 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgggt
     1081 ctcagtgcat aaccttcata cactccacaa tccgaaatgt cttgcctgtt ttgttcttcc
     1141 tccgatcggc tgaagggata gtctttgaaa ggtgatgtgg tcggtgacga ccggcctatt
     1201 tttctttctt ctattgattg ttccaaagcg tacatatttg tagccctgtc tcctcttctc
     1261 tcctctcctc tccttatcga tctgtttgtc cgtttatata gcccctaggc ttgaatattg
     1321 taatacgttc atctgtcttt tcatctagtc tatccaatta ttatacattg cagggtaccc
     1381 acaaaatcac gtgttgcttg ccagccacat atttgcatcc acaagcacag cccactttcg
     1441 ttaccccggc ccgtatagaa acgtcatagt agccaatcaa ttgtatattg gatatcttca
     1501 tcgtgtgttc catcatcgat cgtcgatcgg gcccgttcta gcctgctttc ttcatccctg
     1561 gctttgtctt gccgccagag ttcatgccaa agatgacgat ggagcaatga gagaccgagc
     1621 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1681 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1741 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1801 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1861 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1921 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1981 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     2041 cggtaactat cgtcttgagc ccaacccggt aagacacgac ttatcgccac tggcagcagc
     2101 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2161 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2221 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2281 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2341 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2401 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2461 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2521 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctggctccc
     2581 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2641 accgcgagag ccacgctcac cg
//
