LOCUS       sequence-472732-        3146 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous TR promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdTR
FEATURES             Location/Qualifiers
     source          1..3146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     617..636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(855..959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1027..1045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1083..1105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1205..1224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2381..2969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2461..2480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3140..3146,1..785,786..854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
       61 tggctccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
      121 gcaatgatac cgcgagagcc acgctcaccg gctccagatt tatcagcaat aaaccagcca
      181 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
      241 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
      301 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
      361 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
      421 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
      481 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
      541 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
      601 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
      661 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
      721 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
      781 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
      841 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
      901 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
      961 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     1021 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     1081 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     1141 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     1201 tatgcggcat cagagcagat tgtactgggt ctcagtgcta ttcttcctcc ctaatggaat
     1261 ggatggtgaa ggcaagcaaa gacggagcaa ggaagaaaga agaaagaggg gaagaaagat
     1321 agatccagag agatgcctct gttcaaatag actaaacttc gtcctgcagt ttatctgata
     1381 ctctgtttca accggcccca aatttgaatc tgattgagaa aaatcgtgaa agatgaatga
     1441 gaaatgctca gacttcttct tcggttagcc aatcagatga gcctgacggg gtttttacaa
     1501 tccccaaata ctcccgagcg acttcgccgg ggttataaac tctattcaca tctgagctcg
     1561 aaggcgaaag aagagaaata agatggtgat gagtagaaaa tgaagaaagg aaaggatagg
     1621 gaaaaaagaa gagaatggga aataaaaatt aaaaaaaaag aagagaagga gaagcgatcc
     1681 gacccatgct gaagcttgtt tagtcaagac tgagcgccag aacaaagctc aacgaattct
     1741 catcctatag aaattcacga cagaaagacg gcggaacgac tgtagtaggg aggcatgtct
     1801 atgtgagttc tatctgatag tcagatcact tgctctatct gattaactca agaaaaggag
     1861 aagagtgaac gaaagaagca ggagaggaac aggaatgtca actgggatga taacttaatc
     1921 agattacatc gctatcagtc agtggctttg tcagttcgtt gcaggttcaa gaaccatatg
     1981 ttctataacg gtcaggagac attcttgaag gcagccagca tatacagtca agtagaaaga
     2041 aacgaacaag tctcttgtca ttgatctgag gatgtctcca acccaaggga tgacaacgag
     2101 gcaatcagaa atctgggaaa caccagatcg aatatgactc gggtgaggat tgggaataaa
     2161 gaaaaaatgc cagtaaagtt cgagacacat agctcagctt gagacgcaga aaccacgaca
     2221 gcatcattct tgacccagca atgagagacc gagcggtatc agctcactca aaggcggtaa
     2281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     2341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     2401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     2461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     2521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     2581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     2641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagcccaacc
     2701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     2761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     2821 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     2881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     2941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     3001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     3061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     3121 agtaaacttg gtctgacagt taccaa
//
