LOCUS       sequence-472736-        3146 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous HP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdHP1
FEATURES             Location/Qualifiers
     source          1..3146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     469..488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(707..811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     879..897
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(935..957)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1057..1076
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2233..2821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2313..2332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2992..3146,1..637,638..706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
       61 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
      121 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
      181 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
      241 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
      301 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
      361 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
      421 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
      481 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
      541 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
      601 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
      661 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
      721 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
      781 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      841 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
      901 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
      961 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1021 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactgg
     1081 gtctcagtgc tctatacctc ttctgatcac atcacttgat aagacagagt ctgcgctcta
     1141 ccgattcttt tattttcttg atcctcgatt tttgtaaagt cgagttgtgt ctgtccttga
     1201 aggacggcgt tctttttgtt tctttgaata cagacagcag tgctgcttgt ttcttcctct
     1261 tctttctgat tcattctttt cctgtcttac tccgttatga tatcacgtta ttcccatcag
     1321 actcgtctca agtgaaagag cagatataaa gcgggtcgtc cttattctat acggtataaa
     1381 tgaagcttca cgttcttccg gtcacttgac tagcaacata attaaaacca accaattaca
     1441 cgtgggatgg cggggtaaag ttcatcggcc cgggttagtc cctgacccaa caatatcaag
     1501 ttccagaccg tactaaacgc cgacagtgct tatttctgtc ggagtcctgg agtcccggtg
     1561 ccccggtgcc tgagcttgcc cgcctccgcc ttcctcgcag gctgaacatc gttcgaacaa
     1621 atggctcctt ccagttatat cctgagtaaa caacagaatg aataggacaa acattcggta
     1681 ccaaaacaca catatatggc gcacacccgt acccttagcg cacgcgggtg cactcaagct
     1741 cacaccccaa gacacgtttc ctaaggaaga aggcaaactc agaataccaa ataaaagagg
     1801 ccgaacacct actacccgaa atcgttaggt tcggtgtcga aacgtgcacg ggatagaaga
     1861 gaatgtccct ccgatgaaac agtgcgcgcc tttcggggtc gcaatgaatg tctgagacgg
     1921 aaaactcaac gagaacgttc aaaatgatcg gatgggcgag acaaatctct cctctctttc
     1981 cttctttctc tgtaaaatac acccactctc tttgctttcc ctcgatttct tccccctctt
     2041 tcgttcctca tcgaatatcc tccagaaaga taccgaccct ctcctactca gaatgagaga
     2101 ccgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2521 cttatccggt aactatcgtc ttgagcccaa cccggtaaga cacgacttat cgccactggc
     2581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2641 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3061 gctccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3121 aatgataccg cgagagccac gctcac
//
