LOCUS       sequence-472740-        2655 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the codon-optimized X. dendrorhous NLuc gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpBC-Nluc
FEATURES             Location/Qualifiers
     source          1..2655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(138..726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(218..237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(897..1688,1689..1757))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1520..1539
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1758..1862)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1930..1948
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1986..2008)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2108..2127
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 agagaccgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
       61 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      121 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      181 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      241 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      301 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      361 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      421 tgcgccttat ccggtaacta tcgtcttgag cccaacccgg taagacacga cttatcgcca
      481 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
      541 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
      601 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      661 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      721 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      781 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      841 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      901 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      961 gcctggctcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     1021 gctgcaatga taccgcgaga gccacgctca ccggctccag atttatcagc aataaaccag
     1081 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     1141 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     1201 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     1261 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     1321 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     1381 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     1441 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     1501 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1561 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1621 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1681 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1741 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1801 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1861 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1921 acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt
     1981 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     2041 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     2101 aactatgcgg catcagagca gattgtactg ggtctcaaat ggttttcacc ctcgaagatt
     2161 ttgtggggga ctggagacag accgctggat acaacctcga tcaggttctt gaacagggag
     2221 gcgtctccag tcttttccag aatcttggcg tgtctgtcac ccccattcaa cgaatcgtcc
     2281 tctcgggaga gaacgggctc aagatcgata tccacgtcat cattccttac gagggattga
     2341 gtggagatca gatgggacaa attgaaaaga ttttcaaggt cgtttatcct gttgacgacc
     2401 accactttaa agtcatcctg cattatggca ctctcgttat tgacggagtt acaccgaaca
     2461 tgatcgatta ttttggtagg ccttatgagg gtattgctgt attcgacgga aagaaaatta
     2521 ccgtcactgg tactctctgg aatggtaaca aaattatcga cgagcgactt attaacccgg
     2581 acggttcatt gctgtttcgg gtcactatca atggagtcac tggttggcgg ctctgtgaga
     2641 ggatcctcgc ctaaa
//
