LOCUS       sequence-472741-        3146 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous HAP3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdHAP3
FEATURES             Location/Qualifiers
     source          1..3146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     467..486
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(705..809)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     877..895
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(933..955)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1055..1074
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2231..2819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2311..2330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2990..3146,1..635,636..704))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
       61 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
      121 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
      181 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
      241 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
      301 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
      361 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
      421 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
      481 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
      541 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
      601 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
      661 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
      721 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
      781 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
      841 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
      901 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
      961 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     1021 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgggt
     1081 ctcagtgcta tatttatgca aacaccaagc acaccagaat agatttcaag acaaactcaa
     1141 catctgtagc aacgagagtc aacgatgtaa catgaaatat cttagttgga ctggatggta
     1201 tgtaggactg atagtatgag attggatgct cttcacattg agaagaattt ggcttcacct
     1261 ggatataaag atgctctgac acacttctag aacacagacg atcgaaatat ggttagatat
     1321 ataacagcat atcgtcagta tatcgactgg tatctcatcg caatagaggg atctggtatc
     1381 aatcagcata aagttagatc aaagtgtgtg cttatgaagc gaggaatatt ggtatattca
     1441 gcgatagtat cagccgggca ggtatcgtca gcagagcagg tcgaacaaca ccattggcgt
     1501 gtatatgact atagaattac attcagcact atgttctgtt ctgctatggt tctctcaaat
     1561 gaggatgaaa actctccgag tgcaaaagcg gactctggat gttcttacgt cgtgggtcac
     1621 ataatctgtc gacgccgtct atccgacggc tctccttacg gcgtagcctg acctatatac
     1681 acatgttgta gatccttctg gtaacatact ctatatccat gtcatgacca aacatggacg
     1741 gatgagcact taaagtactc aaacctgtcc gacctgatct gacggccgaa ccccagaggg
     1801 tttagaatca aattcgaatc tggctgacta ccattggcca gacggcgaaa accgattctc
     1861 gaacgttttg atttgtcccc ctctcttttt tccaaggaag tggtttctct tcttcttctc
     1921 attttctatt tctttgccgt ttgtctcttt tcgctatctt ttctgtccga ccacagcctc
     1981 ctggatctct ccctcttcca gatcaatgtc atcaccgtca gcagtgtcat ccctttcccc
     2041 agagacagac tcagatgtcg agccgatagt tccggtcgtc gggaagcaaa atgagagacc
     2101 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     2161 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     2221 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2281 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2341 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2401 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2461 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2521 tatccggtaa ctatcgtctt gagcccaacc cggtaagaca cgacttatcg ccactggcag
     2581 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2641 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2701 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2761 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2821 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2881 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2941 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3001 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctggc
     3061 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3121 tgataccgcg agagccacgc tcaccg
//
