LOCUS       sequence-472742-        2540 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous ETFQO promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdETFQO
FEATURES             Location/Qualifiers
     source          1..2540
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     469..488
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(707..811)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     879..897
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(935..957)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1057..1076
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1627..2215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1707..1726)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2386..2540,1..637,638..706))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
       61 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
      121 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
      181 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
      241 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
      301 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
      361 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
      421 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
      481 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
      541 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
      601 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
      661 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
      721 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
      781 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
      841 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
      901 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
      961 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1021 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactgg
     1081 gtctcagtgc tagacatata cattttttct cttttctttt cacctcatct tgctctaaac
     1141 aaatccttct tctatagact agctatgtag atgtcgagct gtatttgtcc attcaatcct
     1201 ttctataaat gatcatatcc gagtcatctt tttttggtgc tgtggctaac atgaggtcga
     1261 cggtcgattg accaatcaga acaccggttt tgatccaacc gccggggcgc ctccatcaag
     1321 ttttggtcat tttcagtgtt tcgtttttct ttatcttcct ctatttcttt ctcacccgct
     1381 tgcccgatca ttctatccac cttcacacaa actctgaaaa gaacaaacac ggatcggtgc
     1441 atacacggat taagtgttat ctgatcctgg tgagcatcat tcatcaatga gagaccgagc
     1501 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     1561 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     1621 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     1681 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     1741 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     1801 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     1861 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     1921 cggtaactat cgtcttgagc ccaacccggt aagacacgac ttatcgccac tggcagcagc
     1981 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     2041 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     2101 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     2161 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     2221 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     2281 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     2341 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     2401 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctggctccc
     2461 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     2521 accgcgagag ccacgctcac
//
