LOCUS       sequence-472743-        2437 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous SDR promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdSDR
FEATURES             Location/Qualifiers
     source          1..2437
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     466..485
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(704..808)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     876..894
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(932..954)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1054..1073
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(1521..2109)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1601..1620)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2280..2437,1..634,635..703))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
       61 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
      121 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
      181 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
      241 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
      301 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
      361 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
      421 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
      481 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
      541 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
      601 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
      661 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
      721 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
      781 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
      841 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
      901 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      961 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     1021 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgggtc
     1081 tcagtgcatc agaaggaata cagtgcgttc gacgtggatg gaatgaattg aaacggaaag
     1141 ggatggtcgt caggaggagg atgagcaaga cgcgttcaca agacgcgtgt gctgataaat
     1201 tacattctta tctaatcata tcagcgtgtc tgtcgatgac gcttcaactc ccgatcttcg
     1261 atcggttgcg gacgaacacg aacgctcaat ggtttaaaga ggggaggaag aacaaggatg
     1321 cgctctcttt tctcctttcc tatctcaccc actcgccaca tcatctgaat caacacatca
     1381 atgagagacc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1441 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     1501 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1561 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1621 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1681 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1741 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1801 cgctgcgcct tatccggtaa ctatcgtctt gagcccaacc cggtaagaca cgacttatcg
     1861 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1921 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1981 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2041 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2101 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2161 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2221 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2281 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2341 gttgcctggc tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2401 agtgctgcaa tgataccgcg agagccacgc tcaccgg
//
