LOCUS       sequence-472745-        3146 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous E3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdE3
FEATURES             Location/Qualifiers
     source          1..3146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     465..484
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(703..807)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     875..893
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(931..953)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1053..1072
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2229..2817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2309..2328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2988..3146,1..633,634..702))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
       61 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
      121 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
      181 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
      241 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
      301 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
      361 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
      421 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
      481 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
      541 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
      601 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
      661 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
      721 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
      781 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
      841 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
      901 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
      961 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     1021 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgggtct
     1081 cagtgccctg aaatcgagac agaagagcag gctcgagaca agagtggaac aagacctggg
     1141 gaagatatat acaggagatt gtgagagaca ctttacgagg attactacag aagctctata
     1201 tcattgccac agaagctcga tgtcgatatc ttggatgctg tacatccata ggtcgggtac
     1261 tatacgggta caccatcggg acacccacag gcagtacgga tgatacattg agaacacgct
     1321 tacgactaga ctgtttatat gtatattgac actctataca cacatactgt gacacgcacg
     1381 gacgccactc aaatacacat cgatactact acacacacat ttcctccaag tgcactcagt
     1441 gtatgaacag acccaagatc gggaatgtgt tccgctgagt cgatctcgat ctggacgaga
     1501 ggagagagtc gatggatcgg gaaaatgtaa ttttcaggaa caggccatcc gaggcggcgg
     1561 cgaggaggag aaaaaaaaga aaggcaataa ggcaataagg caataaggca ataaggcaat
     1621 aaggcaataa atcaatatgg cggcgccggc agagaagaag ctaataagcc aataagccac
     1681 cgaaaaacac cagggcatca gggcactagg gcgccagtca ttctaatcca ccggccggtg
     1741 acgttgtgga tgatcgatcg agcgagccag ggcaagcctg ctgcgctttt aacctccaga
     1801 cctcccaccg gtcgatccca gttcctgagc ggggagaacg caagaggtcc gtgcgagggt
     1861 gcgtgctcgc taattccgtc tctccaaagg aacaccacca ccagggaaag ctgaagagtt
     1921 tgtcgtttcg tctcttgtct tgactgctct ctgtcgtcca tcatcatcat catagatact
     1981 cacacttaca ttcccactca cacatcatct tactctgtac aacgatacca ttacaagcag
     2041 gtttgactct ctgcttgaaa agatttcgtg aattctgcca tcctcgcaat gagagaccga
     2101 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2161 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     2221 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     2281 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     2341 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     2401 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     2461 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     2521 tccggtaact atcgtcttga gcccaacccg gtaagacacg acttatcgcc actggcagca
     2581 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     2641 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2701 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2761 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2821 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2881 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2941 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3001 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctggctc
     3061 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3121 ataccgcgag agccacgctc accggc
//
