LOCUS       sequence-472749-        2928 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous HP3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdHP3
FEATURES             Location/Qualifiers
     source          1..2928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     468..487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(706..810)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     878..896
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(934..956)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1056..1075
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2014..2602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2094..2113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2773..2928,1..636,637..705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
       61 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      121 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      181 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      241 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      301 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      361 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      421 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      481 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      541 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      601 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      661 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
      721 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      781 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
      841 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
      901 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
      961 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1021 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactggg
     1081 tctcagtgct agttagttac ctcctacaag gtctgataca tcggctttgc tctacccttt
     1141 cttgtggttt gcgtttgttt atcttctgaa ttggtagcct tatcttttga ttttccatct
     1201 gcatcaatta aaggcaaatc aactggggtt tactcccatc gtttgtctct cattctatct
     1261 tattcttatt ttgtcttggc ttgttatgtt ttgttttgtt ttgttttgtt ttgttttgtt
     1321 ttgttttctt ttcttttctt ttgttttctt tccttatccc tcgtcctgtt gtaaacgttt
     1381 ctttcctcct ggtggtatat atgatttatc ctcctcctcc tcttgattgt tttctttcta
     1441 tccaatcggg ccgacaggca atgttacaaa caaaacaaaa aacacatggg gtttaaaaga
     1501 ccgatgagac tgtttcgaac ggagattgct tgtgtgtttc tgtggctgag acggctgaaa
     1561 gctgtgtatg tgtatcgatt gacgttggcc aatcgatttt gcacgtgatc tcgaaactgc
     1621 gcggcggccc tcgtcatcac gatctcaaca accgtctctt ctacttccat cgaaccagaa
     1681 gagtgtgaac catcccgttt tttcttcgcc agttctataa acatgggtct tatgctgccc
     1741 gtctctttct tccttggatg cattcgcttt ccatacataa cgtccgttca agatagacta
     1801 ccaaaggtgc ttcttcagta tacctagccc aggatctctc ctgactctgc ttcgctcgct
     1861 ggtatatgga acaatgagag accgagcggt atcagctcac tcaaaggcgg taatacggtt
     1921 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1981 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2041 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2161 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     2221 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     2281 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagccca acccggtaag
     2341 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     2401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     2461 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     2521 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     2581 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     2641 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     2701 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     2761 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     2821 tcgttcatcc atagttgcct ggctccccgt cgtgtagata actacgatac gggagggctt
     2881 accatctggc cccagtgctg caatgatacc gcgagagcca cgctcacc
//
