LOCUS       sequence-472758-        3147 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous GYG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdGYG
FEATURES             Location/Qualifiers
     source          1..3147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     468..487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(706..810)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     878..896
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(934..956)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1056..1075
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2233..2821)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2313..2332)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2992..3147,1..636,637..705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
       61 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
      121 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
      181 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
      241 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
      301 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
      361 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
      421 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
      481 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
      541 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
      601 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
      661 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
      721 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
      781 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
      841 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
      901 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
      961 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     1021 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactggg
     1081 tctcagtgcc aacgcacaaa cctaatacta catagaaaga ccgacagaca gaacgtaaag
     1141 aagaggaagc caaaggctga accagtgggt accgttcaga cagcatttgc gtcacagaca
     1201 acctatacac gctcaaggat tcaaatagaa gagagatact atgatgtgca ataccaaaga
     1261 cggaaatagc aacgagatgg atgaatctac tcagtggcca acggcgacag cagagtacgc
     1321 ccgagtaaaa gaattgctta cgccagcccc ggaatgcgcc atgctcatac catggtatac
     1381 catcatacct atgcattcat accatacact ttccatgctg ccatctgaat caatttgtcc
     1441 gctgcgctct cagtgtgcga aattactcat ctgatttcgc tgcttccttc ctctgcccaa
     1501 gatggaccgg ccggcgtaat tgagctgtgt cacatgcgat caacatcaaa gtcagctcca
     1561 agattaatcc taaaacagtg ctgatgcaga cggaggcata tatgtcgttg tttttggcat
     1621 gcttgcgcac ctgaaggtgc actacatctt tcatgacgag tgacaaactc tcttcccctt
     1681 tgcttctttc ttttctcttt tcgtgtacca taagctgttc tctctgagtc aggttctgcc
     1741 ttgatctgta catataggtg gttacatcga acacgtctct ttcctcgtat catttaccaa
     1801 ctattgtcta tctgtccttg tcctcttgca gacctagccg ggatcaccga gatgggattg
     1861 aatggacgca ccccaaccgc ggcccaagtt gatcctataa aacccccaaa ttaccccatg
     1921 gcataccgcg atctgggtcc acacacgtga cgacattgct gtcacgtgac ctgccgatcg
     1981 atcgccggat atgaccgctt cccgagttat cgagagaaaa caaaagtgaa aaaaaaaaag
     2041 aagagaatcg tccttcagca tacacaacca tctcgatcaa cccatctaac aaatgagaga
     2101 ccgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2161 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2221 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2281 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2341 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2401 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2461 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2521 cttatccggt aactatcgtc ttgagcccaa cccggtaaga cacgacttat cgccactggc
     2581 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2641 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2701 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2761 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     2821 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     2881 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     2941 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     3001 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     3061 gctccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     3121 aatgataccg cgagagccac gctcacc
//
