LOCUS       sequence-472765-        3146 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  Carries the X. dendrorhous HP2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Tobin EE, Collins JH, Marsan CB, Nadeau GT, Mori K, Lipzen A, Mondo 
            S, Grigoriev IV, Young EM
  TITLE     Omics-driven onboarding of the carotenoid producing red yeast 
            Xanthophyllomyces dendrorhous CBS 6938.
  JOURNAL   Appl Microbiol Biotechnol. 2024 Dec 28;108(1):547. doi: 
            10.1007/s00253-024-13379-w.
  PUBMED    39731599
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol."; date: "2024-12-28"; volume: "108(1):547. 
            doi"; pages: " 10.1007/s00253-024-13379-w"
COMMENT     Sequence Label: p0AmpAB-PXdHP2
FEATURES             Location/Qualifiers
     source          1..3146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     470..489
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(708..812)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     880..898
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(936..958)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1058..1077
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2234..2822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2314..2333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2993..3146,1..638,639..707))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
       61 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
      121 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
      181 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
      241 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
      301 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
      361 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
      421 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
      481 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
      541 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
      601 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
      661 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
      721 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
      781 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
      841 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
      901 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
      961 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     1021 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     1081 ggtctcagtg ccttgcaaga agtcttactc cttctcattt tctctcttcg ccctcttcac
     1141 agcgtggcct tgatgacaac caaatatgga aggaactgag attttttcct ttttttttgc
     1201 tctcactcag ctcaaccaat attttccttg aaaggacaag tcgttttgac atgactatcg
     1261 tgtcatgtct ttctgctcag atgacgcgtc gctgtgtata gaggtaaaga acttagggtt
     1321 aattcaagat ttgggtctat gcatctaata ctacattgct gttcaacatc ctgttggccg
     1381 atatgccagg atcttatgta ctatgtagca aaagcctctg aatagaagga taacaaacaa
     1441 gccaactggc tgttgaaggt cagagagaaa tctctcacag gcccaaggaa acagacttac
     1501 ccaagtataa ttgaccaggg ggaaacatga acttgagata gggatcattc catgagtaac
     1561 tttgaggcca ataattttac atccgaagct atattttgca tccagatgat ctgcgtatgt
     1621 aaccataagc acattggtta cgtttcattt ttccgatcgt tcccccgata tgagttttgg
     1681 ccatcgactt tttcctggct caacgccaac ttggctctgt tcagacccgc cagatccctt
     1741 gattccctgc gttttatggg agaaagtgct gctaaccctg ggcaaaggtc ggatcattca
     1801 ccggcctcgt actctgcctg tatgtcctgg ggctgtccgt tagggaagct ttcctgatac
     1861 ctttctcttc ttttccattt tgtttgtcaa ctgtctttcg ttcccttctc tgctcacaac
     1921 cattgcaaac acatatacgc ccatcaagga acagcacttc ttcctcttct ctgctcaaga
     1981 atccattctg ctttgattga ccactacagt tctaggctgg catttcctct atatttccac
     2041 caacgcccaa gactcttcat aagagaacct tgtcctgacg ttcgttgttc cgaatgagag
     2101 accgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2161 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2221 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2281 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2341 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2401 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2461 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     2521 ccttatccgg taactatcgt cttgagccca acccggtaag acacgactta tcgccactgg
     2581 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     2641 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     2701 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2761 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2821 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2881 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2941 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3001 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3061 ggctccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3121 caatgatacc gcgagagcca cgctca
//
