LOCUS       sequence-472833-       14555 bp    DNA     circular SYN 14-MAY-2025
DEFINITION  Protein expression of FL ScVPS34 and FL ScVPS15-ZZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14555)
  AUTHORS   Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, 
            Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL
  TITLE     Structure and flexibility of the endosomal Vps34 complex reveals the
            basis of its function on membranes.
  JOURNAL   Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365.
  PUBMED    26450213
REFERENCE   2  (bases 1 to 14555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2015-10-9"; volume: "350(6257):aac7365. doi"; pages: " 
            10.1126/science.aac7365"
COMMENT     Sequence Label: pYO359
FEATURES             Location/Qualifiers
     source          1..14555
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        365..466
                     /gene="S. cerevisiae TRP1"
                     /label=TRP1 promoter
     CDS             467..1141
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1244..1699)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1381..1402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1593..1612
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1825..1847
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1839..1856
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1840..1856
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1866..1885
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1866..1884
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1962..2079
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     protein_bind    5356..5473
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     CDS             10177..10197
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10219..10239
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10321..10341
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             10357..10530
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             10531..10704
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     terminator      10727..10914
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(10782..10806)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(10953..10973)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10953..10971)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10992..11008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10992..11008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11005..11027)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11016..11032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11040..11046,11047..11064,11065..11070))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11085..11106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11223..11240)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11394..11982)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11474..11493)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12153..12944,12945..13013))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12776..12795
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13014..13118)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13145..14487
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(14108..14155)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     14528..14546
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc aacgacatta ctatatatat aatataggaa gcatttaata gaacagcatc
      241 gtaatatatg tgtactttgc agttatgacg ccagatggca gtagtggaag atattcttta
      301 ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg gagctttttt gccgattaag
      361 aattaattcg gtcgaaaaaa gaaaaggaga gggccaagag ggagggcatt ggtgactatt
      421 gagcacgtga gtatacgtga ttaagcacac aaaggcagct tggagtatgt ctgttattaa
      481 tttcacaggt agttctggtc cattggtgaa agtttgcggc ttgcagagca cagaggccgc
      541 agaatgtgct ctagattccg atgctgactt gctgggtatt atatgtgtgc ccaatagaaa
      601 gagaacaatt gacccggtta ttgcaaggaa aatttcaagt cttgtaaaag catataaaaa
      661 tagttcaggc actccgaaat acttggttgg cgtgtttcgt aatcaaccta aggaggatgt
      721 tttggctctg gtcaatgatt acggcattga tatcgtccaa ctgcatggag atgagtcgtg
      781 gcaagaatac caagagttcc tcggtttgcc agttattaaa agactcgtat ttccaaaaga
      841 ctgcaacata ctactcagtg cagcttcaca gaaacctcat tcgtttattc ccttgtttga
      901 ttcagaagca ggtgggacag gtgaactttt ggattggaac tcgatttctg actgggttgg
      961 aaggcaagag agccccgaaa gcttacattt tatgttagct ggtggactga cgccagaaaa
     1021 tgttggtgat gcgcttagat taaatggcgt tattggtgtt gatgtaagcg gaggtgtgga
     1081 gacaaatggt gtaaaagact ctaacaaaat agcaaatttc gtcaaaaatg ctaagaaata
     1141 ggttattact gagtagtatt tatttaagta ttgtttgtgc acttgccgat ctatgcggtg
     1201 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt aagcgttaat
     1261 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     1321 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     1381 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     1441 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     1501 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     1561 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     1621 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     1681 gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     1741 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     1801 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     1861 gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc gagcggaccg
     1921 gccggccgga tctttgcaaa gatccaaaaa aagcatgcgt acggattaga agccgccgag
     1981 cgggtgacag ccctccgaag gaagactctc ctccgtgcgt cctcgtcttc accggtcgcg
     2041 ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata
     2101 ctagctttta tggttatgaa gaggaaaatt ggcagtaacc tggccccaca aacctcaaat
     2161 gaacgaatca aattaacaac cagatatctc gactgaaaaa aaaggtttaa accagttccc
     2221 tgaaattatt cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt
     2281 ctgtaaatct atttcttaaa cttcttgaat tctactttta tagttagtct tttttttagt
     2341 tttaaaacac caagaactta gtttcgaata aacacacata aataaaccat ggctctgaac
     2401 aacatcacat tctgtgtctc acaagatctg gatgttcccc tgaaagtgaa aatcaagtca
     2461 ttggaaggac ataagccact gttgaagcca tctcaaaaaa tcctgaaccc tgaattaatg
     2521 ctgatagggt caaatgtatt cccttctagt gatctaattg tgtccttaca ggtatttgat
     2581 aaagagagaa acagaaattt aactcttccg atatacacgc cgtatattcc tttcagaaac
     2641 tctagaacct gggattattg gttgactttg cccatacgta tcaaacagct tacctttagc
     2701 agtcatttac gcattatttt atgggaatac aatggttcca agcaaattcc ctttttcaat
     2761 ctagaaacga gcatctttaa cttaaaagac tgtactttaa aaagagggtt tgaatcctta
     2821 aaatttcgtt atgatgtgat tgaccactgt gaagtggtca ctgataacaa agatcaagaa
     2881 aatttgaata aatattttca gggtgaattc acgagactcc cttggcttga cgaaatcact
     2941 ataagcaaat taaggaaaca acgagaaaat aggacttggc ctcagggcac ctttgtctta
     3001 aacttagaat ttccaatgtt agagcttcct gttgtgttca tcgaaaggga aattatgaat
     3061 actcaaatga atattccaac tttgaagaac aatcctggac taagcacagg cttacgagaa
     3121 ccgaatagga atgaccctca aatcaaaatt tctttggggg acaaatatca ctccacattg
     3181 aagttttacg atcctgacca accaaacaat gatccaatag aggaaaagta taggagattg
     3241 gaaagagcat ctaaaaatgc aaacttggac aagcaagtga agccggacat taaaaagagg
     3301 gactacttga ataaaatcat caactacccc cctggcacta aattaacagc acatgaaaag
     3361 ggttcaatat ggaaatatag gtattatttg atgaacaata aaaaagctct tacaaagtta
     3421 ttacagagta caaatttgag agaagaatcg gaaagagtag aggttttaga actaatggac
     3481 tcatgggcag aaattgatat agacgacgca ttagaactat taggctcaac ttttaaaaac
     3541 ctttcagtga gatcgtacgc cgtaaatagg ctgaagaagg catcagacaa ggagctggaa
     3601 ttgtacttat tacagttggt agaagctgtc tgttttgaga acctgtccac tttttccgac
     3661 aaatctaaca gtgaattcac tattgtggat gccgtatctt cgcaaaagct ttcgggtgat
     3721 tctatgttac tatctacatc gcatgccaac caaaaattat taaagtccat ctcgagtgaa
     3781 tcggaaactt ccgggacaga atcgctacca atcgtcattt ctcccttggc tgagtttttg
     3841 attaggagag cactggtcaa cccaaggtta ggaagtttct tctattggta tttaaagtcc
     3901 gagtctgagg acaagccata tttagaccag attttaagtt ccttttggag tagactggat
     3961 aaaaaatctc ggaatatact gaacgatcaa gtcaggctaa taaatgtgct cagggaatgt
     4021 tgtgaaacaa ttaagaggct gaaagatact acagccaaaa agatggaatt actagtgcat
     4081 ttattggaga cgaaagtcag gcctcttgtc aaagtacgac caattgcttt accactagac
     4141 cctgatgtgt tgatatgcga cgtttgtcct gaaacttcga aggtatttaa aagctcctta
     4201 tcgccactaa aaataacatt caaaacgacc ttaaaccaac catatcactt aatgtttaag
     4261 gttggcgatg atttgagaca agatcaactg gtagtacaga tcataagttt aatgaatgag
     4321 ttattaaaaa acgaaaacgt ggacttgaaa ttgacaccat acaaaatttt ggcaacagga
     4381 ccacaagaag gtgccattga atttatccct aatgacacat tagctagcat actaagcaaa
     4441 tatcacggta ttcttggcta ccttaaactc cactatcctg atgagaacgc gacattaggc
     4501 gtacagggct gggttttaga taattttgtc aaatcttgtg ctggctattg tgttattaca
     4561 tacatcttag gtgtcggcga taggcactta gacaacttac tagtcacacc agatgggcac
     4621 ttttttcacg cagactttgg ttatatcttg ggtcaggacc ccaaaccttt tccgccatta
     4681 atgaaattgc cccctcaaat tatagaggcg tttgggggtg cagaatcatc aaattatgat
     4741 aaatttcgca gctactgttt tgtggcatat tcgattttaa gaaggaatgc aggcttaatc
     4801 ttaaacctgt ttgaattaat gaagacgtcg aacatacctg atatcagaat agatcccaat
     4861 ggtgctatat tacgtgtaag agagagattt aatttgaata tgtccgaaga agatgccaca
     4921 gtgcattttc agaatctaat caatgatagt gtaaatgctt tgctgcctat cgtgattgat
     4981 catttacata atctggcaca atactggcgg acctaagtcg actttgttcc cactgtactt
     5041 ttagctcgta caaaatacaa tatacttttc atttctccgt aaacaacatg ttttcccatg
     5101 taatatcctt ttctattttt cgttccgtta ccaactttac acatacttta tatagctatt
     5161 cacttctata cactaaaaaa ctaagacaat tttaattttg ctgcctgcca tatttcaatt
     5221 tgttataaat tcctataatt tatcctatta gtagctaaaa aaagatgaat gtgaatcgaa
     5281 tcctaagaga attcccctcg aagatcgatc tggcgcccgg gggatctttg caaagatcca
     5341 aaaaaagcat gcgtacggat tagaagccgc cgagcgggtg acagccctcc gaaggaagac
     5401 tctcctccgt gcgtcctcgt cttcaccggt cgcgttcctg aaacgcagat gtgcctcgcg
     5461 ccgcactgct ccgaacaata aagattctac aatactagct tttatggtta tgaagaggaa
     5521 aattggcagt aacctggccc cacaaacctc aaatgaacga atcaaattaa caaccagata
     5581 tctcgactga aaaaaaaggt ttaaaccagt tccctgaaat tattccccta cttgactaat
     5641 aagtatataa agacggtagg tattgattgt aattctgtaa atctatttct taaacttctt
     5701 gaattctact tttatagtta gtcttttttt tagttttaaa acaccaagaa cttagtttcg
     5761 aataaacaca cataaataaa cctcgagatg ggggcacaat tatcactagt ggtccaagca
     5821 tcaccttcca tagccatttt ttcatatatc gatgtcttag aggaagtaca ctacgtttca
     5881 cagttaaact catcaagatt cttaaaaaca tgcaaggcac tggaccctaa cggcgaaatt
     5941 gttatcaaag tgtttattaa accaaaagac caatatagct tacgaccttt tctccaacgt
     6001 ataagggctc aatcgtttaa gttgggacaa ctaccgcacg ttttaaacta cagtaaattg
     6061 atcgagacaa atagagccgg ctacatgata cggcagcact taaaaaataa tttatatgac
     6121 agattgagtt tgagacctta cttacaagac attgaactga aattcattgc tttccagttg
     6181 ttaaatgcat taaaggacat tcataatctg aatattgtcc atggtgatat aaagacagaa
     6241 aatatcctag taacaagttg gaattggtgt atattgacag attttgctgc atttatcaaa
     6301 cccgtatatt tgcctgaaga taatccaggt gaatttttat tctacttcga cacctcgaag
     6361 agaagaacct gttatctagc cccggagagg tttaactcta aactttacca agatggaaaa
     6421 tctaacaatg gtaggctaac taaagaaatg gacatattta gtcttggatg tgttattgca
     6481 gaaatatttg ctgaaggaag acccatcttc aacttatcac agctattcaa atataaaagt
     6541 aattcatatg acgtaaacag ggaatttctc atggaggaaa tgaattctac cgatttaagg
     6601 aacttggttc tagacatgat tcaactagat ccatccaaaa gactttcatg tgatgaacta
     6661 ctgaataaat atcgtggcat tttcttcccc gattatttct acactttcat ttatgattat
     6721 ttcagaaatt tggttactat gacaacaagc acaccgatat cagataacac ttgcaccaat
     6781 agtaccttgg aagacaatgt aaaactttta gatgaaacta cggaaaaaat atacagagat
     6841 ttttcccaaa tatgtcattg tttggacttt cctttaataa aagacggggg tgagattggt
     6901 tcagaccccc caattttgga atcttacaaa atagagatag aaattagtcg gtttttaaac
     6961 acaaacttat atttccccca aaattaccat ttagtcttac agcagtttac caaagtatcc
     7021 gaaaagataa aatcagttaa agaggaatgt gccttactct ttatctctta tttgtctcat
     7081 agtataagaa gtattgtttc gactgctacg aaacttaaaa atttagaact attagcagta
     7141 tttgcacaat tcgtttctga tgaaaataaa attgatcgag tcgtacctta tttcgtatgt
     7201 tgttttgaag atagtgacca ggacgtccag gccctatctt tgttaacatt aatccaggta
     7261 ctcacctctg taagaaaatt gaatcaattg aacgagaata tatttgtgga ctacttactt
     7321 ccgagactga aaagattact tatttccaat aggcagaata ccaattattt aaggattgtg
     7381 tttgctaatt gtttgagcga cttggccatt atcattaata gatttcaaga atttacattt
     7441 gctcagcact gcaatgataa ctcaatggat aacaacacgg aaatcatgga aagcagtacc
     7501 aagtattcag caaaattgat ccaaagtgtc gaagatttaa ctgtctcttt tttaacagat
     7561 aatgatactt atgtaaagat ggcacttttg caaaacattc ttccactttg taaatttttt
     7621 ggtagggaaa gaacaaacga tattatactg agtcatttaa taacctacct taatgataag
     7681 gacccagcat tgcgagtttc cttaattcaa acaatatccg gaatatcaat tcttttgggt
     7741 accgttacat tagaacagta tattttacca ttgttaatcc agaccatcac tgattcggaa
     7801 gaattagtag tgatcagtgt tttacaaagc ttaaaatctt tgttcaagac tgggttgatt
     7861 aggaaaaaat attatattga tatatcaaaa acaacatctc ccttgttgtt gcatcctaat
     7921 aattggataa gacagtttac tttgatgata attatagaaa ttattaataa gttatcaaaa
     7981 gccgaagtgt actgcattct ctatccaata ataaggcctt tcttcgaatt tgacgttgag
     8041 ttcaacttca aatcaatgat aagctgttgc aagcaaccag tgtcaagatc ggtttacaat
     8101 ctattgtgta gttggtctgt tagagcgtca aaatctttat tttggaaaaa aatcatcaca
     8161 aatcatgtag attcattcgg aaataataga atcgaattta taacaaaaaa ttactcaagt
     8221 aaaaattatg gatttaataa aagagatacg aaatcaagtt cctcgctgaa gggtattaaa
     8281 acatcatcca ccgtctattc acatgacaac aaggaaattc ccttaactgc tgaagacaga
     8341 aattggattg ataagttcca cattattggg ctaacagaaa aagatatttg gaaaattgtg
     8401 gctttgaggg gttatgtaat aaggacagcg agagttatgg cagcgaaccc tgattttcca
     8461 tataataata gtaattaccg tccattagta cagaactcac cacctaacct aaaccttacg
     8521 aatattatgc caagaaacat tttctttgat gtagagtttg ctgaagagtc gacaagtgag
     8581 ggacaagatt ctaacttaga gaaccaacag atatataaat atgatgaaag tgagaaagat
     8641 agcaataagc taaatattaa cggtagcaaa cagctatcta ccgtcatgga cataaatgga
     8701 tcactaatat tcaaaaataa gtccattgcc actactactt ctaatttgaa gaacgttttt
     8761 gttcagttag aaccaacgtc ctatcacatg cattctccaa atcatggctt gaaagataat
     8821 gcaaatgtta aaccagaaag gaaggtagtc gtcagcaaca gctatgaagg cgacgttgaa
     8881 agcatagaaa aattcctatc gactttcaaa attttacctc ctctgagaga ttataaggag
     8941 tttgggccta ttcaagagat tgtacggagt ccaaacatgg gtaatttgag gggcaagttg
     9001 atagctactt tgatggaaaa cgaacccaat tctattacgt cttctgctgt ttctccagga
     9061 gaaacaccct atttaataac aggttcagat caaggtgtaa tcaagatttg gaacctgaaa
     9121 gagattatcg tgggcgaggt ttactcttct tctttaactt atgactgctc ctctaccgta
     9181 actcagataa ccatgattcc taactttgac gcgtttgccg tttccagtaa agatggacaa
     9241 ataattgtat taaaggttaa tcattaccaa caagaaagtg aagtcaaatt tttgaattgc
     9301 gaatgcatca ggaaaattaa cttgaagaat tttggtaaaa atgaatacgc agtgagaatg
     9361 agagcatttg tgaatgagga aaaatctcta ctagtagcat tgacgaattt gtcaagggtt
     9421 attatatttg atattagaac cctggagagg ttacaaatta tagagaattc tccaaggcat
     9481 ggtgccgttt caagcatctg tatcgatgaa gagtgttgtg tcctaatttt ggggacgact
     9541 agaggtatta ttgatatatg ggatatccgt ttcaacgtgc tgataaggag ttggtccttt
     9601 ggggaccacg caccaatcac gcatgtggag gtttgtcagt tttatggaaa gaattctgta
     9661 attgttgtag gaggtagttc aaaaacattt ctaacaatat ggaattttgt taaggggcat
     9721 tgtcagtatg ctttcataaa ttctgatgaa cagccatcta tggagcactt tttaccaatt
     9781 gagaaaggct tagaagaatt aaatttttgt ggaatcaggt ctttaaacgc actaagcact
     9841 atctcagtat ctaatgataa aattcttctt accgatgaag caacaagttc cattgttatg
     9901 tttagcctaa atgagctttc ttcttctaaa gcagtaataa gtccttcaag attcagtgac
     9961 gtttttattc ctacacaagt tacggcaaat ctcacaatgt tattgagaaa aatgaaacgt
    10021 actagcactc attcagtaga tgattctcta tatcatcatg atattataaa ttctatatct
    10081 acatgtgaag ttgatgagac acctttgctg gttgcttgtg ataactcagg gcttattgga
    10141 atcttccaag gatcctctag accaactact gcctctgaaa atttgtactt tcaaggcgag
    10201 ttgaagacca ctgcttctga aaacttgtac ttccaaggtg agttactcga gggtgaattg
    10261 aagactgctg ctttggctca acacgacgaa gctagatccg gagcaggggc gggtgctagc
    10321 gagaacctgt acttccaggg cgcccacgat gaagccgtag acaacaaatt caacaaagaa
    10381 caacaaaacg cgttctatga gatcttacat ttacctaact taaacgaaga acaacgaaac
    10441 gccttcatcc aaagtttaaa agatgaccca agccaaagcg ctaacctttt agcagaagct
    10501 aaaaagctaa atgatgctca ggcgccgaaa gtagacaaca aattcaacaa agaacaacaa
    10561 aacgcgttct atgagatctt acatttacct aacttaaacg aagaacaacg aaacgccttc
    10621 atccaaagtt taaaagatga cccaagccaa agcgctaacc ttttagcaga agctaaaaag
    10681 ctaaatgatg ctcaggcgcc gaaagtagac gcgaattcgt gagctcgcga atttcttatg
    10741 atttatgatt tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag
    10801 tgactcttag gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag
    10861 gttgctttct caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcagcgg
    10921 ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
    10981 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    11041 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    11101 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    11161 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    11221 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    11281 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    11341 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    11401 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    11461 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    11521 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    11581 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    11641 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    11701 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    11761 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    11821 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    11881 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    11941 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    12001 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    12061 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    12121 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    12181 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    12241 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    12301 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    12361 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    12421 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    12481 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    12541 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    12601 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    12661 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    12721 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    12781 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    12841 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    12901 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    12961 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    13021 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    13081 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    13141 cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
    13201 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
    13261 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
    13321 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
    13381 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
    13441 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
    13501 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
    13561 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
    13621 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
    13681 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
    13741 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
    13801 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
    13861 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
    13921 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
    13981 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
    14041 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
    14101 gcgctctgaa gttcctatac tttctagaga ataggaactt cggaatagga acttcaaagc
    14161 gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac agctcactgt
    14221 tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga agaacggcat
    14281 agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg atgaaaggta
    14341 gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc ttccttcagc
    14401 actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct catccttcaa
    14461 tgctatcatt tcctttgata ttggatcata ctaagaaacc attattatca tgacattaac
    14521 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
