LOCUS       sequence-472880-       11539 bp    DNA     circular SYN 14-MAY-2025
DEFINITION  To express ICP0 (doxycycline inducible) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11539)
  TITLE     Manel lab plasmids 2025
REFERENCE   2  (bases 1 to 11539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pEasiLV-MCS-coICP0
FEATURES             Location/Qualifiers
     source          1..11539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4137
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     polyA_signal    4147..4371
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(4385..5062)
                     /codon_start=1
                     /product="far-red noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2009)"
                     /label=E2-Crimson
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGVGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFFYGSKAYIKHPADIPDYLKQSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTLIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERNYPRDGVLKGENHM
                     ALKLKGGGHYLCEFKSIYMAKKPVKLPGYHYVDYKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(4645..4664)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(5069..5122)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(join(5147..5152,5153..5188,5189..5191,5192..5227,
                     5228..5230,5231..5266,5267..5272,5273..5890))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    5902..6454
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5904..6472
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6064..6083)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6293..6310
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(9052..9076)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(9073..9111)
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    join(9144..9162,9163..9185,9186..9204,9205..9227,9228..9246,
                     9247..9269,9270..9288,9289..9311,9312..9330,9331..9353,
                     9354..9372,9373..9395,9396..9414)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     misc_feature    9435..9526
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(9467..9486)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    9540..9588
                     /note="synthetic polyadenylation signal"
     misc_feature    9601..10189
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9654..9674)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10072..10083)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10276..10509
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10581..10702
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10618..10637)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10672..10691
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10742..10877
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10897..10916)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10898..10916)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10926..10943)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10926..10942)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10935..10957)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11084..11512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11171..11190)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11381..11402
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttata
     4141 gatatcccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
     4201 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
     4261 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
     4321 aaggcacggg ggaggggcaa acaacagatg gctggaaact agaaggcaca gtcgagacac
     4381 tagtctactg gaacaggtgg tggcgggcct cggcgcgctc gtactgctcc accacggtgt
     4441 agtcctcgtt gtgggaggtg atgtcgagct tgtagtccac gtagtggtag ccgggcagct
     4501 tcacgggctt cttggccatg tagatggact tgaactcaca caggtagtgg ccgccgccct
     4561 tcagcttcag cgccatgtgg ttctcgccct tcagcacgcc gtcgcggggg tagttgcgct
     4621 cagtggaggg ctcccagccc agagtcttct tctgcattac ggggccgtcg gaggggaagt
     4681 tcacgccgat gaacttcacg tggtagatga gggtgccgtc ctgcagggag gagtcctggg
     4741 tcacggtcac cacgccgccg tcctcgaagt tcatcacgcg ctcccacttg aagccctcgg
     4801 ggaaggactg cttgaggtag tcggggatgt cggcggggtg cttgatgtac gccttggagc
     4861 cgtagaagaa ctggggggac aggatgtccc aggcgaaggg cagggggccg cccttggtca
     4921 cttgcagctt ggcggtctgg gtgccctcgt agggcttgcc ctcgcccacg ccctcgatct
     4981 cgaactcgtg gccgttcacg gagccctcca tgtgcacctt gaagcgcatg aagggcttga
     5041 tgacgttctc agtgctatcc atgtcgactg ggccaggatt ctcctcgacg tcaccgcatg
     5101 ttagcagact tcctctgccc tctccactgc cacgcgttcc tccgcccccg gggagcatgt
     5161 caaggtcaaa atcgtcaaga gcgtcagcag gcagcatatc aaggtcaaag tcgtcaaggg
     5221 catcggctgg gagcatgtct aagtcaaaat cgtcaagggc gtcggtcggc ccgccgcttt
     5281 cgcactttag ctgtttctcc aggccacata tgattagttc caggccgaaa aggaaggcag
     5341 gttcggctcc ctgccggtcg aacagctcaa ttgcttgtct cagaagtggg ggcatagaat
     5401 cggtggtagg tgtctctctt tcctcttttg ctacttgatg ctcctgttcc tccaatacgc
     5461 agcccagtgt aaagtggccc acggcggaca gagcgtacag tgcgttctcc agggagaagc
     5521 cttgctgaca caggaacgcg agctgatttt ccagggtttc gtactgtttc tctgttgggc
     5581 gggtgccgag atgcacttta gccccgtcgc gatgtgagag gagagcacag cggtatgact
     5641 tggcgttgtt ccgcagaaag tcttgccatg actcgccttc cagggggcag aagtgggtat
     5701 gatgcctgtc cagcatctcg attggcaggg catcgagcag ggcccgcttg ttcttcacgt
     5761 gccagtacag ggtaggctgc tcaactccca gcttttgagc gagtttcctt gtcgtcaggc
     5821 cttcgatacc gacaccattg agtaattcca gagctccgtt tatgactttg ctcttgtcca
     5881 gtctagacat ggttgtggcc atattatcat cgtgtttttc aaaggaaaac cacgtccccg
     5941 tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac
     6001 cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg
     6061 ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg
     6121 cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg
     6181 cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt
     6241 cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct
     6301 ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg
     6361 gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac
     6421 cggccttatt ccaagcggct tcggccagta acgttagggg ggggggggcg gaattctaga
     6481 gtcgcggccg cctacacatt gatcctagca gaagcacagg ctgcagatgc atctcgagtc
     6541 actgctttcc ctcgtctctt gtggaagcgc cttgctcgcc ggaccaagga tgtctgctcc
     6601 tcagagatct aaaatccaga gcgccgacga gggttccttg gtcgaacagc atattgccca
     6661 cgggtgtcat ccagaggcta ttccattcgc tagcgggggc tgtggggtac tcgggaggca
     6721 tcacgtggtt tccggctgtc tcggggagga gtgtccttct agaccagccg ggcacggcag
     6781 ctctcagtct ggtagccatg ttgccggtct gatccaccag caccacatag gcgccaatgt
     6841 ttccggtctc catgtcgagg atgggcagac aatctccggt gatggttttg ttcacgtagg
     6901 ggctcagagc caccacgctg ctgactccgc tgataggcag ataccttgtc agtcctccgg
     6961 cgggcacagc gccgctggtc tcagcatgtc ttgtctttct agcgcacttt ctggggcccc
     7021 tagggccgct agcggctcta ggtcccagag aggtttcctc ttgtcttcct ccgagggctc
     7081 cgacagctcc tccgctggaa gcgctagcgc tgctgctagc agctccggca gcgctgctag
     7141 cggctccagc gggtgtagga gcgctgctgg agctggcgga gctggagcta gcggaagcgg
     7201 cggaggatgt cagaggtgtt ccgggtccgc cttggcctct gccaccgggg ccggaatcgc
     7261 taggagggtg tgtggcggct cttttagcgc cggcgggagc gaggggaggt cttgtggact
     7321 gagggctggg gttctcttgg ccggagcccc ttctctttct aggcctcacg gcggcttctt
     7381 gctccacgct agcggcggaa ggcagaggag cggctccgga aggagcagct cctctgctag
     7441 gtccgtgtcc gccttccacg ccgggtcctc cgctgcctct agcgactgta gccctagctc
     7501 tgcacagaga ttgctcttga ttggcagctt gagccagaga gctatgggac tggggcactc
     7561 ttctagcatc ggcgggtctg gcggcgggtt cagctccggg ggcgggggcc ctaggtccgg
     7621 ggccgggagg aggggctctc acgcaaggag cgacagcagc cctaggtctg gcgggtccgc
     7681 tggcagcagc gctggcggga gggccgggcc taggggcgct gccgggcata ggagcggcgg
     7741 gaggcctatg aggggaggcg ggggggctgt cgctgatcac aatggggtct ctattgttag
     7801 cgagaggagc gggtctgttc accagaggtc cggttcttcc tctgggcctt ccggcttcgg
     7861 cctccaccac tcccactccg actccgactc ctccgctagg tccggaagct cctctagggg
     7921 cggcagctct gctttgtctg ctgccgccgc ctccggagct attggtggtg gtgtttgtgt
     7981 tgctgcttcc gtggggtcca atgggagcgc tagggggagc tgtcctagca gcggcgggtt
     8041 gaggggcagc atggctggct ccgccggtca cgggaggagc ggcagcgcct ctcctaggag
     8101 gagctctagg tgttctccta ggggcggggg gcacgtagtc ggcatcatcg aggtcgtcgt
     8161 cgtcttcgtc tgttgtaggc tcggggtgtg taggggacag agccctcact gtgtgtcctc
     8221 cgagggtcag atatctagga gcaaacctct gattgccggt ccagatgaaa tccacggctg
     8281 tgccggccct cacagcttct tcagcttcca ttcttgtttg ggggtcgttg acgataggaa
     8341 tggtgctaaa ggagccgcta ggtgtcactc cgacgatcag atacaccagt ttagcgttac
     8401 acagagggca tgtgtttctg agctgcatcc atgtcttcat gcaagggatg cagaatctat
     8461 gcatgcaagg aaaggtgtca cacctcagat gaggggcgat ctcgtctgtg cacacggcgc
     8521 acacatctcc ttcgtcgctt cctccgtcct ctctgggagg agcgcctcca cagcttccgg
     8581 ggtcttcctc tctaggaggg ctgccgccgg acacagctcc tccatcgacg ccttgagggc
     8641 ccagcagtcc ggtctcgaag agctcggtat ctgtggaatc ggcctcgcta gcggagtcgt
     8701 catcgtggtg gtcagcgtcg cctcttcctc ccacctctgt ctcagcttcg gaatcgctgc
     8761 tatcggggag gtccctatca caagggaaga cccacacgtc gggggcgggt tctctttggg
     8821 gtcttccctc gggtcttctt gtgctggctc cgggtctagg ctccatggat ccaccggttt
     8881 aattaagttt aaacgacaaa gtctacgtac tcgagtacgt agactttgtc gtttaaactt
     8941 aattaaaccg gtggatcccc gggtaccgag ctcgaattcg gggccgcgga ggctggatcg
     9001 gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg cgtctccagg cgatctgacg
     9061 gttcactaaa cgagctctgc ttatataggc ctcccaccgt acacgcctac ctcgacccgg
     9121 gtaccgagct cgactttcac ttttctctat cactgatagg gagtggtaaa ctcgactttc
     9181 acttttctct atcactgata gggagtggta aactcgactt tcacttttct ctatcactga
     9241 tagggagtgg taaactcgac tttcactttt ctctatcact gatagggagt ggtaaactcg
     9301 actttcactt ttctctatca ctgataggga gtggtaaact cgactttcac ttttctctat
     9361 cactgatagg gagtggtaaa ctcgactttc acttttctct atcactgata gggagtggta
     9421 aactcgatat cgatagagaa atgttctggc acctgcactt gcactgggga cagcctattt
     9481 tgctagtttg ttttgtttcg ttttgttttg atggagagcg tatgttagta ctatcaattc
     9541 acacaaaaaa ccaacacaca gatgtaatga aaataaagat attttattgc tagtctcgac
     9601 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9661 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9721 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9781 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9841 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     9901 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9961 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
    10021 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
    10081 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
    10141 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
    10201 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
    10261 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
    10321 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    10381 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10441 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10501 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
    10561 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
    10621 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    10681 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
    10741 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    10801 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    10861 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
    10921 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    10981 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    11041 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
    11101 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
    11161 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
    11221 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
    11281 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
    11341 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
    11401 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
    11461 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
    11521 atattaacgc ttacaattt
//
