LOCUS       sequence-472938-        9937 bp    DNA     circular SYN 15-MAY-2025
DEFINITION  Expression in BL21(DE3) of WT T4 DNA polymerase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9937)
  TITLE     Escherichia phage T4 Expression Constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTYB3-T4gp43(WT)
FEATURES             Location/Qualifiers
     source          1..9937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..139
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(140..208,209..1000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(358..377)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(1042..1555)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1237..1258)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1449..1468
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      1666..2254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2155..2174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2408..2425
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2447..2466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2607..2629
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2623..2814)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3371..4453)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4415..4434
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4454..4531)
                     /gene="lacI"
                     /label=lacI promoter
     terminator      4684..4770
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4867..4953
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5050..5136
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5233..5319
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     5454..5473
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5454..5472
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5473..5497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5512..5534
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8236..9054
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae VMA1"
                     /product="5' region of the intein from the yeast Vma1 
                     subunit of the vacuolar ATPase (Chong et al., 1998)"
                     /label=Sce VMA intein 5' region
                     /translation="CFAKGTNVLMADGSIECIENIEVGNKVMGKDGRPREVIKLPRGRE
                     TMYSVVQKSQHRAHKSDSSREVPELLKFTCNATHELVVRTPRSVRRLSRTIKGVEYFEV
                     ITFEMGQKKAPDGRIVELVKEVSKSYPISEGPERANELVESYRKASNKAYFEWTIEARD
                     LSLLGSHVRKATYQTYAPILYENDHFFDYMQKSKFHLTIEGPKVLAYLLGLWIGDGLSD
                     RATFSVDSRDTSLMERVTEYAEKLNLCAEYKDRKEPQVAKTVNLYSKVVRG"
     CDS             9510..9521
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             9631..9786
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     primer_bind     complement(9851..9869)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      9865..9912
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aactacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
       61 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      121 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      181 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      241 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      301 ccttgagagt tttcgccccg aagaacgttt cccaatgatg agcactttta aagttctgct
      361 atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
      421 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      481 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      541 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      601 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      661 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      721 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      781 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      841 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      901 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
      961 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1021 atatatactt tagattgatt taccccggtt gataatcaga aaagccccaa aaacaggaag
     1081 attgtataag caaatattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     1141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     1201 tcaaaagaat agcccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     1261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     1321 ctacgtgaac catcacccaa atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     1381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     1441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     1501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag
     1561 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1621 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1681 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1741 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1801 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1861 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1921 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1981 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2041 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2101 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2161 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2221 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2281 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2341 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2401 cgagcgcagc gagtcagtga gcgaggaagc tatggtgcac tctcagtaca atctgctctg
     2461 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     2521 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     2581 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     2641 atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gcagcgattc
     2701 acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt
     2761 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc
     2821 ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat
     2881 gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa
     2941 acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcc
     3001 gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt
     3061 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
     3121 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
     3181 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt
     3241 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
     3301 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
     3361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     3421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
     3481 tcaccagtga gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
     3541 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
     3601 gcgggatata acatgagctg tcttcggtat cgtcgtatcc cactaccgag atatccgcac
     3661 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
     3721 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
     3781 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
     3841 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
     3901 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
     3961 cttcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
     4021 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
     4081 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
     4141 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
     4201 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
     4261 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
     4321 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
     4381 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
     4441 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
     4501 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac
     4561 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
     4621 aatggtgcat gccggcatgc cgccctttcg tcttcaagaa ttaattccca attccccagg
     4681 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     4741 tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag
     4801 caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggaa ttaattcccc
     4861 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     4921 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     4981 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gaattaattc
     5041 cccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     5101 tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt
     5161 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggaattaa
     5221 ttccccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     5281 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
     5341 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggaat
     5401 tggggatcgg aattaattcc cggtttaaac cggggatctc gatcccgcga aattaatacg
     5461 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
     5521 ctttaagaag gagatataca tatgaaagaa ttttatatct ctattgaaac agtcggaaat
     5581 aacattgttg aacgttatat tgatgaaaat ggaaaggaac gtacccgtga agtagaatat
     5641 cttccaacta tgtttaggca ttgtaaggaa gagtcaaaat acaaagacat ctatggtaaa
     5701 aactgcgctc ctcaaaaatt tccatcaatg aaagatgctc gagattggat gaagcgaatg
     5761 gaagacatcg gtctcgaagc tctcggtatg aacgatttta aactcgctta tataagtgat
     5821 acatatggtt cagaaattgt ttatgaccga aaatttgttc gtgtagctaa ctgtgacatt
     5881 gaggttactg gtgataaatt tcctgaccca atgaaagcag aatatgaaat tgatgctatc
     5941 actcattacg attcaattga cgatcgtttt tatgttttcg accttttgaa ttcaatgtac
     6001 ggttcagtat caaaatggga tgcaaagtta gctgctaagc ttgactgtga aggtggcgat
     6061 gaagttcctc aagaaattct tgaccgagta atttatatgc cattcgataa tgagcgtgat
     6121 atgctcatgg aatatatcaa tctttgggaa cagaaacgac ctgctatttt tactggttgg
     6181 aatattgagg ggtttgacgt tccgtatatc atgaatcgtg ttaaaatgat tctgggtgaa
     6241 cgtagtatga aacgtttctc tccaatcggt cgggtaaaat ctaaactaat tcaaaatatg
     6301 tacggtagca aagaaattta ttctattgat ggcgtatcta ttcttgatta tttagatttg
     6361 tacaagaaat tcgcttttac taatttgccg tcattctctt tggaatcagt tgctcaacat
     6421 gaaaccaaaa aaggtaaatt accatacgac ggtcctatta ataaacttcg tgagactaat
     6481 catcaacgat acattagtta taacatcatt gacgtagaat cagttcaagc aatcgataaa
     6541 attcgtgggt ttatcgatct agttttaagt atgtcttatt acgctaaaat gcctttttct
     6601 ggtgtaatga gtcctattaa aacttgggat gctattattt ttaactcatt gaaaggtgaa
     6661 cataaggtta ttcctcaaca aggttcgcac gttaaacaga gttttccggg tgcatttgtg
     6721 tttgaaccta aaccaattgc acgtcgatac attatgagtt ttgacttgac gtctctgtat
     6781 ccgagcatta ttcgccaggt taacattagt cctgaaacta ttcgtggtca gtttaaagtt
     6841 catccaattc atgaatatat cgcaggaaca gctcctaaac cgagtgatga atattcttgt
     6901 tctccgaatg gatggatgta tgataaacat caagaaggta tcattccaaa ggaaatcgct
     6961 aaagtatttt tccagcgtaa agactggaaa aagaaaatgt tcgctgaaga aatgaatgcc
     7021 gaagctatta aaaagattat tatgaaaggc gcagggtctt gttcaactaa accagaagtt
     7081 gaacgatatg ttaagttcag tgatgatttc ttaaatgaac tatcgaatta caccgaatct
     7141 gttctcaata gtctgattga agaatgtgaa aaagcagcta cacttgctaa tacaaatcag
     7201 ctgaaccgta aaattctcat taacagtctt tatggtgctc ttggtaatat tcatttccgt
     7261 tactatgatt tgcgaaatgc tactgctatc acaattttcg gccaagtcgg tattcagtgg
     7321 attgctcgta aaattaatga atatctgaat aaagtatgcg gaactaatga tgaagatttc
     7381 attgcagcag gtgatactga ttcggtatat gtttgcgtag ataaagttat tgaaaaagtt
     7441 ggtcttgacc gattcaaaga gcagaacgat ttggttgaat tcatgaatca gttcggtaag
     7501 aaaaagatgg aacctatgat tgatgttgca tatcgtgagt tatgtgatta tatgaataac
     7561 cgcgagcatc tgatgcatat ggaccgtgaa gctatttctt gccctccgct tggttcaaag
     7621 ggcgttggtg gattttggaa agcgaaaaag cgttatgctc tgaacgttta tgatatggaa
     7681 gataagcgat ttgctgaacc gcatctaaaa atcatgggta tggaaactca gcagagttca
     7741 acaccaaaag cagtgcaaga agctctcgaa gaaagtattc gtcgtattct tcaggaaggt
     7801 gaagagtctg tccaagaata ctacaagaac ttcgagaaag aatatcgtca acttgactat
     7861 aaagttattg ctgaagtaaa aactgcgaac gatatagcga aatatgatga taaaggttgg
     7921 ccaggattta aatgcccgtt ccatattcgt ggtgtgctaa cttatcgtcg agctgttagc
     7981 ggtttaggtg tagctccaat tttggatgga aataaagtaa tggttcttcc attacgtgaa
     8041 ggaaatccat ttggtgacaa gtgcattgct tggccatcgg gtacagaact tccaaaagaa
     8101 attcgttctg atgtgctatc ttggattgac cactcaactt tgttccaaaa atcgtttgtt
     8161 aaaccgcttg cgggtatgtg tgaatcggct ggcatggact atgaagaaaa agcttcgtta
     8221 gacttcctgt ttggctgctt tgccaagggt accaatgttt taatggcgga tgggtctatt
     8281 gaatgtattg aaaacattga ggttggtaat aaggtcatgg gtaaagatgg cagacctcgt
     8341 gaggtaatta aattgcccag aggaagagaa actatgtaca gcgtcgtgca gaaaagtcag
     8401 cacagagccc acaaaagtga ctcaagtcgt gaagtgccag aattactcaa gtttacgtgt
     8461 aatgcgaccc atgagttggt tgttagaaca cctcgtagtg tccgccgttt gtctcgtacc
     8521 attaagggtg tcgaatattt tgaagttatt acttttgaga tgggccaaaa gaaagccccc
     8581 gacggtagaa ttgttgagct tgtcaaggaa gtttcaaaga gctacccaat atctgagggg
     8641 cctgagagag ccaacgaatt agtagaatcc tatagaaagg cttcaaataa agcttatttt
     8701 gagtggacta ttgaggccag agatctttct ctgttgggtt cccatgttcg taaagctacc
     8761 taccagactt acgctccaat tctttatgag aatgaccact ttttcgacta catgcaaaaa
     8821 agtaagtttc atctcaccat tgaaggtcca aaagtacttg cttatttact tggtttatgg
     8881 attggtgatg gattgtctga cagggcaact ttttcggttg attccagaga tacttctttg
     8941 atggaacgtg ttactgaata tgctgaaaag ttgaatttgt gcgccgagta taaggacaga
     9001 aaagaaccac aagttgccaa aactgttaat ttgtactcta aagttgtcag aggtaatggt
     9061 attcgcaata atcttaatac tgagaatcca ttatgggacg ctattgttgg cttaggattc
     9121 ttgaaggacg gtgtcaaaaa tattccttct ttcttgtcta cggacaatat cggtactcgt
     9181 gaaacatttc ttgctggtct aattgattct gatggctatg ttactgatga gcatggtatt
     9241 aaagcaacaa taaagacaat tcatacttct gtcagagatg gtttggtttc ccttgctcgt
     9301 tctttaggct tagtagtctc ggttaacgca gaacctgcta aggttgacat gaatgtcacc
     9361 aaacataaaa ttagttatgc tatttatatg tctggtggag atgttttgct taacgttctt
     9421 tcgaagtgtg ccggctctaa aaaattcagg cctgctcccg ccgctgcttt tgcacgtgag
     9481 tgccgcggat tttatttcga gttacaagaa ttgaaggaag acgattatta tgggattact
     9541 ttatctgatg attctgatca tcagtttttg cttggatccc aggttgtcgt ccatgcatgc
     9601 ggtggcctga ccggtctgaa ctcaggcctc acgacaaatc ctggtgtatc cgcttggcag
     9661 gtcaacacag cttatactgc gggacaattg gtcacatata acggcaagac gtataaatgt
     9721 ttgcagcccc acacctcctt ggcaggatgg gaaccatcca acgttcctgc cttgtggcag
     9781 cttcaatgac tgcaggaagg ggatccggct gctaacaaag cccgaaagga agctgagttg
     9841 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     9901 aggggttttt tgctgaaagg aggaactata tccggat
//
