LOCUS       sequence-472946-        9132 bp    DNA     circular SYN 15-MAY-2025
DEFINITION  Expresses Sr62TK-Kinase1 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9132)
  AUTHORS   Chen R, Chen J, Outram MA, Powell OR, Arndell T, Gajendiran K, Wang 
            YL, Ayliffe MA, Blundell C, Figueroa M, Sperschneider J, Vanhercke 
            T, Tang D, Xu Y, Zhong G, Gardener C, Yu G, Gourdoupis S, Jaremko Ł,
            Matny O, Steffenson BJ, Boshoff WHP, Meyer WB, Arold ST, Dodds PN, 
            Wulff BBH
  TITLE     A wheat tandem kinase sensor activates an NLR helper to trigger 
            immunity
  JOURNAL   bioRxiv 2024.08.30.610287
REFERENCE   2  (bases 1 to 9132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.08.30.610287"
COMMENT     Sequence Label: pGADT7_Sr62TK-Kinase1
FEATURES             Location/Qualifiers
     source          1..9132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(396..1187,1188..1256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1019..1038
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1257..1361)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1429..1447
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1485..1507)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     1607..1626
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      1643..2807
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        3581..4285
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             4331..4351
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4367..4708
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     primer_bind     4714..4733
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4714..4732
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4739..4748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4751..4777
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     terminator      6370..6557
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(6425..6449)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(6674..7768)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIEATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7769..8174)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(8216..8232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8216..8232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8229..8251)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8240..8256
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8264..8270,8271..8288,8289..8294))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8309..8330
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    8385..8418
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    8496..8529
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(8598..8615)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8769..9132,1..225))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8849..8868)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
       61 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
      421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
      481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
      541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
      601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
      661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
      721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
      781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
      841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
      901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
      961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1381 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     1441 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     1501 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     1561 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     1621 attgtactga gagtgcacca taacgcattt aagcataaac acgcactatg ccgttcttct
     1681 catgtatata tatatacagg caacacgcag atataggtgc gacgtgaaca gtgagctgta
     1741 tgtgcgcagc tcgcgttgca ttttcggaag cgctcgtttt cggaaacgct ttgaagttcc
     1801 tattccgaag ttcctattct ctagctagaa agtataggaa cttcagagcg cttttgaaaa
     1861 ccaaaagcgc tctgaagacg cactttcaaa aaaccaaaaa cgcaccggac tgtaacgagc
     1921 tactaaaata ttgcgaatac cgcttccaca aacattgctc aaaagtatct ctttgctata
     1981 tatctctgtg ctatatccct atataaccta cccatccacc tttcgctcct tgaacttgca
     2041 tctaaactcg acctctacat tttttatgtt tatctctagt attactcttt agacaaaaaa
     2101 attgtagtaa gaactattca tagagtgaat cgaaaacaat acgaaaatgt aaacatttcc
     2161 tatacgtagt atatagagac aaaatagaag aaaccgttca taattttctg accaatgaag
     2221 aatcatcaac gctatcactt tctgttcaca aagtatgcgc aatccacatc ggtatagaat
     2281 ataatcgggg atgcctttat cttgaaaaaa tgcacccgca gcttcgctag taatcagtaa
     2341 acgcgggaag tggagtcagg ctttttttat ggaagagaaa atagacacca aagtagcctt
     2401 cttctaacct taacggacct acagtgcaaa aagttatcaa gagactgcat tatagagcgc
     2461 acaaaggaga aaaaaagtaa tctaagatgc tttgttagaa aaatagcgct ctcgggatgc
     2521 atttttgtag aacaaaaaag aagtatagat tctttgttgg taaaatagcg ctctcgcgtt
     2581 gcatttctgt tctgtaaaaa tgcagctcag attctttgtt tgaaaaatta gcgctctcgc
     2641 gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa tagcgctttc
     2701 gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa aattagcgct
     2761 ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttgct tgcatgcctg
     2821 caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg
     2881 aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg
     2941 tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca tgctgactct
     3001 gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc
     3061 ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg agattggaga
     3121 agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg gtgtcattat
     3181 ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa gaatgagcca
     3241 agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat gaccttgaag
     3301 gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg gttgctacca
     3361 gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt acgctttcaa
     3421 ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac acttctccta
     3481 tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac ccctccgcgc
     3541 tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt tgtttccggg
     3601 tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga ttcctataat
     3661 accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat agaacagata
     3721 ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc attgatggtg
     3781 gtacataacg aactaatact gtagccctag acttgatagc catcatcata tcgaagtttc
     3841 actacccttt ttccatttgc catctattga agtaataata ggcgcatgca acttcttttc
     3901 tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa
     3961 tgatggaaga cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt
     4021 tccagagctg atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc
     4081 acactactct ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat
     4141 aaaaaaaagt ttgctgtctt gctatcaagt ataaatagac ctgcaattat taatcttttg
     4201 tttcctcgtc attgttctcg ttccctttct tccttgtttc tttttctgca caatatttca
     4261 agctatacca agcatacaat caactccaag ctttgcaaag atggataaag cggaattaat
     4321 tcccgagcct ccaaaaaaga agagaaaggt cgaattgggt accgccgcca attttaatca
     4381 aagtgggaat attgctgata gctcattgtc cttcactttc actaacagta gcaacggtcc
     4441 gaacctcata acaactcaaa caaattctca agcgctttca caaccaattg cctcctctaa
     4501 cgttcatgat aacttcatga ataatgaaat cacggctagt aaaattgatg atggtaataa
     4561 ttcaaaacca ctgtcacctg gttggacgga ccaaactgcg tataacgcgt ttggaatcac
     4621 tacagggatg tttaatacca ctacaatgga tgatgtatat aactatctat tcgatgatga
     4681 agatacccca ccaaacccaa aaaaagagat ctttaatacg actcactata gggcgagcgc
     4741 cgccatggag tacccatacg acgtaccaga ttacgctcat atggccatgg aggccagtga
     4801 attcatggac ggatacgagt tcgagagggc ggagatagat gcactggagc gcgtcgtacg
     4861 ggatccaaga gaaaagccaa taagcctgtc gttttggctt ctcaggcgca taacgaatga
     4921 tttctctaac gcatctgaaa ttggtcgagg tggattttcg aacgtttacc tgggggtgct
     4981 tccaagtggg ttgcgtattg ctgtcaaaaa gctttaccaa cttactgcat tggctaatga
     5041 atttgaagat gaagttctta ccacaatgag ggttgctcac aaaaacatag tgcgactcat
     5101 aggctactgt cgtcacacgc aatctgaagt cttcgaatta aaaggagaat ctattttcgc
     5161 acaggtcaga gaaaggttga tctgtacaga gtatgtgcct aatggaactc ttgatggaca
     5221 tatcactgat aagtttcatg gaggacttga ttgggaccag cgttatcaag ttctcaaagg
     5281 aatttgtcac ggtttgcatt atctgcacga tgaaatacgc gttatccaca gagatatcaa
     5341 aacagaaaat atattgctag atgataacgg cgtgccaaaa gtttttgact ttggtttttc
     5401 gaagtgcctt cctgatggag aatctttaat taaagatgaa gcaacccagg gaaccctagg
     5461 gtatttggcg ccggagtcag ttgataagca agaatattcc tttaagtccg acatatacag
     5521 ctttggtgtt gtgatcatac aattattgac tggatcgtgg atcagtaaga accgaaatgt
     5581 tgagctagaa cttaaggagt tgagaaaaag gttggtaaga gaaggagcat ttccatcatg
     5641 ggaaaacaaa taccaccaag ttagaacatg tctggagatt gtgtataact gcatggacat
     5701 cgacccagat aaaaggccga ctgcattgga aattatccaa cggcttgatg aaaccgaaag
     5761 cgcgaactat tctgttagca gtggccatgc aacaccactt tggcagtcag gagatgaaga
     5821 atccgactca gcggaagtgg atgcttttga gccagagaga acaccatcaa aagacattct
     5881 ttcagatgat gaggaacccg catcagcaga cgcaaatgga gaaacgagca cacaaaaaca
     5941 taatatgcct gatcttgtaa gtaaacagtc aggatccatc gagctcgagc tgcagatgaa
     6001 tcgtagatac tgaaaaaccc cgcaagttca cttcaactgt gcatcgtgca ccatctcaat
     6061 ttctttcatt tatacatcgt tttgccttct tttatgtaac tatactcctc taagtttcaa
     6121 tcttggccat gtaacctctg atctatagaa ttttttaaat gactagaatt aatgcccatc
     6181 ttttttttgg acctaaattc ttcatgaaaa tatattacga gggcttattc agaagctttg
     6241 gacttcttcg ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg
     6301 ccagaaattt acgaaaagat ggaaaagggt caaatcgttg gtagatacgt tgttgacact
     6361 tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa
     6421 ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg
     6481 agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt
     6541 gaccacacct ctaccggccg gtcgaaattc ccctacccta tgaacatatt ccattttgta
     6601 atttcgtgtc gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa
     6661 aaagagaatc tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt
     6721 cggtggtact gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt
     6781 taactgcatc ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca
     6841 ttgcagcaga caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag
     6901 aaccgtggca tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg
     6961 acgcagatgg caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat
     7021 caccaaacat gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga
     7081 tcatggcggc agaatcaatc aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga
     7141 tggtttcctc cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca
     7201 aggaccaaat aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga
     7261 ttctttgcac ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt
     7321 cttcctttct cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag
     7381 tacctttagc aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt
     7441 tacatggtct taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt
     7501 caggtctaac actacctgta ccccatttag gaccacccac agcacctaac aaaacggcat
     7561 cagccttctt ggaggcttcc agcgcctcat ctggaagtgg gacacctgta gcttcgatag
     7621 cagcaccacc aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa
     7681 tagctttaag aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca
     7741 aaacgacgat cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat
     7801 attgctgaaa tgtaaaaggt aagaaaagtt agaaagtaag acgattgcta accacctatt
     7861 ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga tgcaagcatt
     7921 tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcgctct cacctttcct
     7981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     8041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     8101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     8161 gtattcccac agttggggga tctcgactct agctagagga tcaattcgta atcatgtcat
     8221 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8281 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8341 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgataact tcgtataatg
     8401 tatgctatac gaagttatta ggtctgaaga ggagtttacg tccagccaag ctagcttggc
     8461 tgcaggtcga gcggccgcga tccggaaccc ttaatataac ttcgtataat gtatgctata
     8521 cgaagttatc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     8581 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     8641 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     8701 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     8761 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     8821 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     8881 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     8941 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9001 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9061 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9121 gccactggta ac
//
