LOCUS       sequence-473041-       10004 bp    DNA     circular SYN 16-MAY-2025
DEFINITION  Expresses the m6A-binding-deficient mutant of YTHDF2 with synonymous
            mutations for resistance against sgYTHDF2-1-mediated knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Chen Z, Zeng C, Yang L, Che Y, Chen M, Sau L, Wang B, Zhou K, Chen 
            Y, Qing Y, Shen C, Zhang T, Wunderlich M, Wu D, Li W, Wang K, Leung 
            K, Sun M, Tang T, He X, Zhang L, Swaminathan S, Mulloy JC, Muschen 
            M, Huang H, Weng H, Xiao G, Deng X, Chen J
  TITLE     YTHDF2 promotes ATP synthesis and immune evasion in B cell 
            malignancies.
  JOURNAL   Cell. 2024 Dec 12:S0092-8674(24)01324-2. doi: 
            10.1016/j.cell.2024.11.007.
  PUBMED    39694037
REFERENCE   2  (bases 1 to 10004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Dec 12:S0092-8674(24)01324-2. doi: 10.1016/j.cell.2024.11.007."
COMMENT     Sequence Label: pCDH-3_Flag-YTHDF2-m(6)A-MUT (nonsense mutatio...
FEATURES             Location/Qualifiers
     source          1..10004
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2013..2216
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2167..2187
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2213..2237
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2290..2355
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        4146..4357
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4370..4638
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      4667..4676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4697..5359
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     CDS             5429..5482
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5483..6082
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5483..5502)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5979..5999
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6083..6671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6136..6156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6554..6565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6745..6978
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7050..7171
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7087..7106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7141..7160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7211..7346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7379..7395)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7379..7395)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7392..7414)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7403..7419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7427..7433,7434..7451,7452..7457))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7472..7493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7610..7627)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7781..8369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8540..9331,9332..9400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9163..9182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9401..9505)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9573..9591
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9629..9651)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9751..9770
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9964..9986
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9978..9995
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9979..9995
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagtattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1981 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2041 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2101 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2161 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ttatataagc agagctcgtt
     2221 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagat
     2281 tctagaatgg attacaaaga ccatgacggt gattataaag atcatgacat cgactacaag
     2341 gatgacgatg acaaggctag cgaattctcg gccagcagcc tcttggagca gagaccaaaa
     2401 ggtcaaggaa acaaagtaca aaatggatct gtacatcaaa aggatggatt aaacgatgat
     2461 gattttgaac cttacttgag tccacaggca aggcccaata atgcatatac tgccatgtca
     2521 gattcctact tacccagtta ctacagtccc tccattggct tctcctattc tttgggtgaa
     2581 gctgcttggt ctacgggggg tgacacagcc atgccctact taacttctta tggacagctg
     2641 agcaacggag agccccactt cctaccagat gcaatgtttg ggcaaccagg agccctaggt
     2701 agcactccat ttcttggtca gcatggtttt aatttctttc ccagtgggat tgacttctca
     2761 gcatggggaa ataacagttc tcagggacag tctactcaga gctctggata tagtagcaat
     2821 tatgcttatg cacctagctc cttaggtgga gccatgattg atggacagtc agcttttgcc
     2881 aatgagaccc tcaataaggc tcctggcatg aatactatag accaagggat ggcagcactg
     2941 aagttgggta gcacagaagt tgcaagcaat gttccaaaag ttgtaggttc tgctgttggt
     3001 agcgggtcca ttactagtaa catcgtggct tccaatagtt tgcctccagc caccattgct
     3061 cctccaaaac cagcatcttg ggctgatatt gctagcaagc ctgcaaaaca gcaacctaaa
     3121 ctgaagacca agaatggcat tgcagggtca agtcttccgc cacccccgat aaagcataac
     3181 atggatattg gaacttggga taacaagggt cccgttgcaa aagccccctc acaggctttg
     3241 gttcagaata taggtcagcc aacccagggg tctcctcagc ctgtaggtca gcaggctaac
     3301 aatagcccac cagtggctca ggcatcagta gggcaacaga cacagccatt gcctccacct
     3361 ccaccacagc ctgcccagct ttcagtccag caacaggcag ctcagccaac ccgctgggtg
     3421 gctccacgca atcgcggaag tgggttcggt cataatgggg tggatggtaa tggagtagga
     3481 cagtctcagg ctggttctgg atctactcct tcagaacccc acccagtgtt ggagaagctt
     3541 cggtccatta ataactataa ccccaaagat tttgactgga atctgaaaca tggccgggtt
     3601 ttcatcatta agagctactc tgaggacgat attcaccgtt ccattaagta taatattgcc
     3661 tgcagcacag agcatggtaa caagagactg gatgctgctt atcgttccat gaacgggaaa
     3721 ggccccgttt acttactttt cagtgtcaac ggcagtggac acttctgtgg cgtggcagaa
     3781 atgaaatctg ctgtggacta caacacatgt gcaggtgtgg cctcccagga caaatggaag
     3841 ggtcgttttg atgtcaggtg gatttttgtg aaggacgttc ccaatagcca actgcgacac
     3901 attcgcctag agaacaacga gaataaacca gtgaccaact ctagggacac tcaggaagtg
     3961 cctctggaaa aggctaagca ggtgttgaaa attatagcca gctacaagca caccacttcc
     4021 atttttgatg acttctcaca ctatgagaaa cgccaagagg aagaagaaag tgttaaaaag
     4081 gaacgtcaag gtcgtgggaa ataagcggcc gcgaaggatc tgcgatcgct ccggtgcccg
     4141 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     4201 ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     4261 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     4321 cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag gggctcgcat
     4381 ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt tgagtcgcgt
     4441 tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa
     4501 gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta gactcagccg
     4561 gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt cgttttctgt
     4621 tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca ccatggagag
     4681 cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca ccctgaacgg
     4741 cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc gcatgaccaa
     4801 caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga gccacgtgat
     4861 gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc ccttcctgca
     4921 cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg acggcggcgt
     4981 gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg acttcaaggt
     5041 ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca tccgcagcaa
     5101 cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca gcttcgcccg
     5161 caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc acatgcactt
     5221 caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg ccttccgccg
     5281 cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc acgccttcaa
     5341 gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg tggacggcac
     5401 cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa catgcggtga
     5461 cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5521 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5581 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5641 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     5701 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5761 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5821 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5881 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5941 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     6001 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     6061 gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg aaagattgac
     6121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6241 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6301 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6361 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6421 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6481 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6541 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6601 tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct ccctttgggc
     6661 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     6721 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agataagatc
     6781 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6841 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6901 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6961 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg
     7021 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca
     7081 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     7141 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct
     7201 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     7261 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     7321 gtagtgagga ggcttttttg gaggcctaga cttttgcaga gaccaaattc gtaatcatgt
     7381 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7441 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7501 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     7561 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     7621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     7681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     7741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     8041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     8101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     8161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     8221 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     8281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     8341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     8401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     8461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     9001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     9061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     9121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     9181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     9241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     9301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     9361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     9421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
     9541 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
     9601 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     9661 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     9721 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     9781 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
     9841 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9901 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     9961 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg
//
