LOCUS       sequence-473153-        8405 bp    DNA     circular SYN 19-MAY-2025
DEFINITION  Transcription factor TCF7L1 with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8405)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 8405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-TCF7L1-Brd 453
FEATURES             Location/Qualifiers
     source          1..8405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             312..545
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    617..738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(654..673)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     708..727
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      778..913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(933..952)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(934..952)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(962..979)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(962..978)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(971..993)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1120..1575
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1207..1226)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1417..1438
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1601..1705
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1706..1774,1775..2566)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1924..1943)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2737..3325
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3226..3245
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3479..3496
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3613..3634
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3649..3654,3655..3672,3673..3679)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3687..3703
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3692..3714
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3711..3727
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3711..3727
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3746..3766
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3748..3766
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3794..4020
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4021..4201
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4248..4373
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4866..5099
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5284..5328
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5595..5712
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5746..6152
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    join(8195..8405,1..193)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
ORIGIN
        1 gctctgtgtt tgctgacgca acccccactg gctggggcat tgccaccacc tgtcaactcc
       61 tttctgggac tttcgctttc cccctcccga tcgccacggc agaactcatc gccgcctgcc
      121 ttgcccgctg ctggacaggg gctaggttgc tgggcactga taattccgtg gtgttgtcgg
      181 ggaagctgac gtcctttgca attcgatatc aagctgtacc ttctagatgt acagcacaga
      241 gagcgcttct agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
      301 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
      361 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      421 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      481 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      541 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
      601 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      661 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
      721 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
      781 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      841 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
      901 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      961 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1021 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1081 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     1141 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1201 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     1261 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     1321 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1381 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1441 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1501 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1561 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1621 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1681 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     1741 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     1801 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1861 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1921 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1981 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2041 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2101 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2161 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2221 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2281 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2341 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2401 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2461 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2521 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2581 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2641 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     2701 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     2761 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     2821 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2881 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2941 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3001 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3061 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3121 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3181 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3241 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3301 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3361 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3421 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3481 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3541 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3601 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     3661 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     3721 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     3781 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3841 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3901 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3961 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     4021 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4081 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4141 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4201 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     4261 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     4321 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     4381 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     4441 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     4501 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     4561 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     4621 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     4681 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     4741 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     4801 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4861 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4921 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4981 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     5041 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     5101 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     5161 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     5221 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     5281 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     5341 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     5401 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     5461 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     5521 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     5581 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     5641 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     5701 attcaaaatt ttatcgatca cgagactagc ctcgagctgg ctgcagtaac gccattttgc
     5761 aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc gggtacatga
     5821 aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc cccggcccgg
     5881 ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga acagatggtc
     5941 cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt ccaggctccc
     6001 ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct gcttctcgct
     6061 tctgttcgcg cgcttctgct tcccgagctc tataaaagag ctcacaaccc ctcactcggc
     6121 gcgccagtcc tccgacagac tgagtcggcc ggtcgaatca agcttatcga taccgtcgac
     6181 ggatccgcta gcccgcggtt cgaaatgccg cagttaggtg gggggggtgg aggaggtggg
     6241 gggggatctg gtggtggtgg tggatcatct gcaggagctg ctggcggtgg agatgatctt
     6301 ggtgctaatg atgaactcat tccatttcaa gatgaaggcg gggaagaaca agaacctagt
     6361 tctgactccg ctagtgctca aagagatctc gatgaagtga aatcaagttt agtgaatgaa
     6421 tcagaaaatc aaagttccag ttcagattct gaagccgaaa gaaggcccca acctgttagg
     6481 gatacctttc aaaagccccg cgattacttt gctgaggttc ggagacccca agattcagcc
     6541 ttcttcaagg ggccacctta tccaggctat ccttttctta tgattccaga tcttagttca
     6601 ccatatctga gtaatggtcc actctcccca ggcggggccc ggacgtattt acaaatgaag
     6661 tggccactgc tggacgttcc ttcttctgct accgttaaag atactcggtc tcccagcccc
     6721 gcccatctca gcaacaaggt cccagtggta caacatcctc accatatgca cccattgacc
     6781 ccgctgatta cgtattcaaa cgatcatttt agcccaggaa gcccgcctac acatctgtct
     6841 cctgaaattg accctaaaac cggtatacca cgcccacccc atcccagtga actcagccca
     6901 tactatcctt tgagtcctgg cgccgtgggt cagattcctc atccattagg ttggttggtt
     6961 ccgcaacagg gacaaccaat gtattcactg ccgcctggcg ggtttaggca tccctatcct
     7021 gctttagcta tgaatgcttc aatgagctct cttgtttcat cacgcttttc cccacatatg
     7081 gtagcgcccg cacatccagg tcttcctacg tctggaattc cacatcccgc gatagtgtca
     7141 ccaattgtga aacaagagcc agcgccacca agtctctcac ccgctgtctc agtcaagtct
     7201 cctgttacag tcaagaaaga agaagagaag aaacctcatg taaagaaacc cctcaacgct
     7261 tttatgctgt acatgaaaga aatgcgtgca aaagtcgtcg cggaatgtac attgaaagag
     7321 agcgccgcga tcaatcaaat tttgggtcgg aaatggcata atctcagcag agaggagcaa
     7381 gcaaaatatt atgaactcgc aaggaaagaa aggcaactgc attcacaact gtatcccact
     7441 tggagtgcta gagataatta cggcaagaag aagaaacgga aacgcgagaa acaattatca
     7501 caaacgcaaa gccaacaaca ggtgcaagaa gctgaaggag ctttggcttc taaatccaag
     7561 aaaccgtgcg tgcaatattt gccacctgaa aagccttgcg attctccagc atcaagtcat
     7621 ggctcaatgc tcgattctcc agctacccca agtgccgcgc tcgcaagccc cgccgctccc
     7681 gccgcaactc actctgaaca ggcgcaacca cttagtttga ctactaagcc tgagactcgc
     7741 gcacaattag cattacattc agcagcgttt ctctctgcca aagccgccgc aagctcaagc
     7801 ggacaaatgg gaagtcaacc accattgctc agcagaccac ttccattagg ctcaatgcca
     7861 actgccttgc tcgcaagtcc cccatctttt ccagcaaccc tgcacgctca tcaagcatta
     7921 ccagtcctgc aagcgcaacc gctgagtttg gtgacaaaga gtgcacatta aaccggtcgg
     7981 atcccccggg ctgcaggaat tcgagcatct taccgccatt tattcccata tttgttctgt
     8041 ttttcttgat ttgggtatac atttaaatgt taataaaaca aaatggtggg gcaatcattt
     8101 acatttttag ggatatgtaa ttactagttc aggtgtattg ccacaagaca aacatgttaa
     8161 gaaactttcc cgttatttac gctctgttcc tgttaatcaa cctctggatt acaaaatttg
     8221 tgaaagattg actgatattc ttaactatgt tgctcctttt acgctgtgtg gatatgctgc
     8281 tttaatgcct ctgtatcatg ctattgcttc ccgtacggct ttcgttttct cctccttgta
     8341 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtccgtc aacgtggcgt
     8401 ggtgt
//
