LOCUS       sequence-473338-       16650 bp    DNA     circular SYN 06-JUN-2025
DEFINITION  Express MLL1-NanoLuc(R) Fusion Protein in Mammalian Cells under a 
            CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16650)
  TITLE     Promega Early Access Collection
REFERENCE   2  (bases 1 to 16650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NanoBRET MLL1/WDR5 Assay Donor Vector, MLL1
FEATURES             Location/Qualifiers
     source          1..16650
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             12993..13505
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(13593..13611)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    13648..13769
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13659..13678)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     13713..13732
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        14014..14371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        14014..14205
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(14037..14057)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      14222..14357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        14386..14433
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             14465..15259
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14519..14538)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     15129..15148
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    15323..15371
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(15610..16198)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15690..15709)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    16314..16597
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggcg cacagctgtc
     1081 ggtggcgctt ccccgcccga cccgggacca ccgggggtgg cggcggcggg gggcgccggg
     1141 gcctaggggg cgccccgcgg caacgcgtcc cggccctgct gcttcccccc gggcccccgg
     1201 tcggcggtgg cggccccggg gcgcccccct cccccccggc tgtggcggcc gcggcggcgg
     1261 cggcgggaag cagcggggct ggggttccag ggggagcggc cgccgcctca gcagcctcct
     1321 cgtcgtccgc ctcgtcttcg tcttcgtcat cgtcctcagc ctcttcaggg ccggccctgc
     1381 tccgggtggg cccgggcttc gacgcggcgc tgcaggtctc ggccgccatc ggcaccaacc
     1441 tgcgccggtt ccgggccgtg tttggggaga gcggcggggg aggcggcagc ggagaggatg
     1501 agcaattctt aggttttggc tcagatgaag aagtcagagt gcgaagtccc acaaggtctc
     1561 cttcagttaa aactagtcct cgaaaacctc gtgggagacc tagaagtggc tctgaccgaa
     1621 attcagctat cctctcagat ccatctgtgt tttcccctct aaataaatca gagaccaaat
     1681 ctggagataa gatcaagaag aaagattcta aaagtataga aaagaagaga ggaagacctc
     1741 ccaccttccc tggagtaaaa atcaaaataa cacatggaaa ggacatttca gagttaccaa
     1801 agggaaacaa agaagatagc ctgaaaaaaa ttaaaaggac accttctgct acgtttcagc
     1861 aagccacaaa gattaaaaaa ttaagagcag gtaaactctc tcctctcaag tctaagttta
     1921 agacagggaa gcttcaaata ggaaggaagg gggtacaaat tgtacgacgg agaggaaggc
     1981 ctccatcaac agaaaggata aagacccctt cgggtctcct cattaattct gaactggaaa
     2041 agccccagaa agtccggaaa gacaaggaag gaacacctcc acttacaaaa gaagataaga
     2101 cagttgtcag acaaagccct cgaaggatta agccagttag gattattcct tcttcaaaaa
     2161 ggacagatgc aaccattgct aagcaactct tacagagggc aaaaaagggg gctcaaaaga
     2221 aaattgaaaa agaagcagct cagctgcagg gaagaaaggt gaagacacag gtcaaaaata
     2281 ttcgacagtt catcatgcct gttgtcagtg ctatctcctc gcggatcatt aagacccctc
     2341 ggcggtttat agaggatgag gattatgacc ctccaattaa aattgcccga ttagagtcta
     2401 caccgaatag tagattcagt gccccgtcct gtggatcttc tgaaaaatca agtgcagctt
     2461 ctcagcactc ctctcaaatg tcttcagact cctctcgatc tagtagcccc agtgttgata
     2521 cctccacaga ctctcaggct tctgaggaga ttcaggtact tcctgaggag cggagcgata
     2581 cccctgaagt tcatcctcca ctgcccattt cccagtcccc agaaaatgag agtaatgata
     2641 ggagaagcag aaggtattca gtgtcggaga gaagttttgg atctagaacg acgaaaaaat
     2701 tatcaactct acaaagtgcc ccccagcagc agacctcctc gtctccacct ccacctctgc
     2761 tgactccacc gccaccactg cagccagcct ccagtatctc tgaccacaca ccttggctta
     2821 tgcctccaac aatcccctta gcatcaccat ttttgcctgc ttccactgct cctatgcaag
     2881 ggaagcgaaa atctattttg cgagaaccga catttaggtg gacttcttta aagcattcta
     2941 ggtcagagcc acaatacttt tcctcagcaa agtatgccaa agaaggtctt attcgcaaac
     3001 caatatttga taatttccga ccccctccac taactcccga ggacgttggc tttgcatctg
     3061 gtttttctgc atctggtacc gctgcttcag cccgattgtt ttcgccactc cattctggaa
     3121 caaggtttga tatgcacaaa aggagccctc ttctgagagc tccaagattt actccaagtg
     3181 aggctcactc tagaatattt gagtctgtaa ccttgcctag taatcgaact tctgctggaa
     3241 catcttcttc aggagtatcc aatagaaaaa ggaaaagaaa agtgtttagt cctattcgat
     3301 ctgaaccaag atctccttct cactccatga ggacaagaag tggaaggctt agtagttctg
     3361 agctctcacc tctcaccccc ccgtcttctg tctcttcctc gttaagcatt tctgttagtc
     3421 ctcttgccac tagtgcctta aacccaactt ttacttttcc ttctcattcc ctgactcagt
     3481 ctggggaatc tgcagagaaa aatcagagac caaggaagca gactagtgct ccggcagagc
     3541 cattttcatc aagtagtcct actcctctct tcccttggtt taccccaggc tctcagactg
     3601 aaagagggag aaataaagac aaggcccccg aggagctgtc caaagatcga gatgctgaca
     3661 agagcgtgga gaaggacaag agtagagaga gagaccggga gagagaaaag gagaataagc
     3721 gggagtcaag gaaagagaaa aggaaaaagg gatcagaaat tcagagtagt tctgctttgt
     3781 atcctgtggg tagggtttcc aaagagaagg ttgttggtga agatgttgcc acttcatctt
     3841 ctgccaaaaa agcaacaggg cggaagaagt cttcatcaca tgattctggg actgatatta
     3901 cttctgtgac tcttggggat acaacagctg tcaaaaccaa aatacttata aagaaaggga
     3961 gaggaaatct ggaaaaaacc aacttggacc tcggcccaac tgccccatcc ctggagaagg
     4021 agaaaaccct ctgcctttcc actccttcat ctagcactgt taaacattcc acttcctcca
     4081 taggctccat gttggctcag gcagacaagc ttccaatgac tgacaagagg gttgccagcc
     4141 tcctaaaaaa ggccaaagct cagctctgca agattgagaa gagtaagagt cttaaacaaa
     4201 ccgaccagcc caaagcacag ggtcaagaaa gtgactcatc agagacctct gtgcgaggac
     4261 cccggattaa acatgtctgc agaagagcag ctgttgccct tggccgaaaa cgagctgtgt
     4321 ttcctgatga catgcccacc ctgagtgcct taccatggga agaacgagaa aagattttgt
     4381 cttccatggg gaatgatgac aagtcatcaa ttgctggctc agaagatgct gaacctcttg
     4441 ctccacccat caaaccaatt aaacctgtca ctagaaacaa ggcaccccag gaacctccag
     4501 taaagaaagg acgtcgatcg aggcggtgtg ggcagtgtcc cggctgccag gtgcctgagg
     4561 actgtggtgt ttgtactaat tgcttagata agcccaagtt tggtggtcgc aatataaaga
     4621 agcagtgctg caagatgaga aaatgtcaga atctacaatg gatgccttcc aaagcctacc
     4681 tgcagaagca agctaaagct gtgaaaaaga aagagaaaaa gtctaagacc agtgaaaaga
     4741 aagacagcaa agagagcagt gttgtgaaga acgtggtgga ctctagtcag aaacctaccc
     4801 catcagcaag agaggatcct gccccaaaga aaagcagtag tgagcctcct ccacgaaagc
     4861 ccgtcgagga aaagagtgaa gaagggaatg tctcggcccc tgggcctgaa tccaaacagg
     4921 ccaccactcc agcttccagg aagtcaagca agcaggtctc ccagccagca ctggtcatcc
     4981 cgcctcagcc acctactaca ggaccgccaa gaaaagaagt tcccaaaacc actcctagtg
     5041 agcccaagaa aaagcagcct ccaccaccag aatcaggtcc agagcagagc aaacagaaaa
     5101 aagtggctcc ccgcccaagt atccctgtaa aacaaaaacc aaaagaaaag gaaaaaccac
     5161 ctccggtcaa taagcaggag aatgcaggca ctttgaacat cctcagcact ctctccaatg
     5221 gcaatagttc taagcaaaaa attccagcag atggagtcca caggatcaga gtggacttta
     5281 aggaggattg tgaagcagaa aatgtgtggg agatgggagg cttaggaatc ttgacttctg
     5341 ttcctataac acccagggtg gtttgctttc tctgtgccag tagtgggcat gtagagtttg
     5401 tgtattgcca agtctgttgt gagcccttcc acaagttttg tttagaggag aacgagcgcc
     5461 ctctggagga ccagctggaa aattggtgtt gtcgtcgttg caaattctgt cacgtttgtg
     5521 gaaggcaaca tcaggctaca aagcagctgc tggagtgtaa taagtgccga aacagctatc
     5581 accctgagtg cctgggacca aactacccca ccaaacccac aaagaagaag aaagtctgga
     5641 tctgtaccaa gtgtgttcgc tgtaagagct gtggatccac aactccaggc aaagggtggg
     5701 atgcacagtg gtctcatgat ttctcactgt gtcatgattg cgccaagctc tttgctaaag
     5761 gaaacttctg ccctctctgt gacaaatgtt atgatgatga tgactatgag agtaagatga
     5821 tgcaatgtgg aaagtgtgat cgctgggtcc attccaaatg tgagaatctt tcagatgaga
     5881 tgtatgagat tctatctaat ctgccagaaa gtgtggccta cacttgtgtg aactgtactg
     5941 agcggcaccc tgcagagtgg cgactggccc ttgaaaaaga gctgcagatt tctctgaagc
     6001 aagttctgac agctttgttg aattctcgga ctaccagcca tttgctacgc taccggcagg
     6061 ctgccaagcc tccagactta aatcccgaga cagaggagag tataccttcc cgcagctccc
     6121 ccgaaggacc tgatccacca gttcttactg aggtcagcaa acaggatgat cagcagcctt
     6181 tagatctaga aggagtcaag aggaagatgg accaagggaa ttacacatct gtgttggagt
     6241 tcagtgatga tattgtgaag atcattcaag cagccattaa ttcagatgga ggacagccag
     6301 aaattaaaaa agccaacagc atggtcaagt ccttcttcat tcggcaaatg gaacgtgttt
     6361 ttccatggtt cagtgtcaaa aagtccaggt tttgggagcc aaataaagta tcaagcaaca
     6421 gtgggatgtt accaaacgca gtgcttccac cttcacttga ccataattat gctcagtggc
     6481 aggagcgaga ggaaaacagc cacactgagc agcctccttt aatgaagaaa atcattccag
     6541 ctcccaaacc caaaggtcct ggagaaccag actcaccaac tcctctgcat cctcctacac
     6601 caccaatttt gagtactgat aggagtcgag aagacagtcc agagctgaac ccacccccag
     6661 gcatagaaga caatagacag tgtgcgttat gtttgactta tggtgatgac agtgctaatg
     6721 atgctggtcg tttactatat attggccaaa atgagtggac acatgtaaat tgtgctttgt
     6781 ggtcagcgga agtgtttgaa gatgatgacg gatcactaaa gaatgtgcat atggctgtga
     6841 tcaggggcaa gcagctgaga tgtgaattct gccaaaagcc aggagccacc gtgggttgct
     6901 gtctcacatc ctgcaccagc aactatcact tcatgtgttc ccgagccaag aactgtgtct
     6961 ttctggatga taaaaaagta tattgccaac gacatcggga tttgatcaaa ggcgaagtgg
     7021 ttcctgagaa tggatttgaa gttttcagaa gagtgtttgt ggactttgaa ggaatcagct
     7081 tgagaaggaa gtttctcaat ggcttggaac cagaaaatat ccacatgatg attgggtcta
     7141 tgacaatcga ctgcttagga attctaaatg atctctccga ctgtgaagat aagctctttc
     7201 ctattggata tcagtgttcc agggtatact ggagcaccac agatgctcgc aagcgctgtg
     7261 tatatacatg caagatagtg gagtgccgtc ctccagtcgt agagccggat atcaacagca
     7321 ctgttgaaca tgatgaaaac aggaccattg cccatagtcc aacatctttt acagaaagtt
     7381 catcaaaaga gagtcaaaac acagctgaaa ttataagtcc tccatcacca gaccgacctc
     7441 ctcattcaca aacctctggc tcctgttatt atcatgtcat ctcaaaggtc cccaggattc
     7501 gaacacccag ttattctcca acacagagat cccctggctg tcgaccgttg ccttctgcag
     7561 gaagtcctac cccaaccact catgaaatag tcacagtagg tgatccttta ctctcctctg
     7621 gacttcgaag cattggctcc aggcgtcaca gtacctcttc cttatcaccc cagcggtcca
     7681 aactccggat aatgtctcca atgagaactg ggaatactta ctctaggaat aatgtttcct
     7741 cagtctccac caccgggacc gctactgatc ttgaatcaag tgccaaagta gttgatcatg
     7801 tcttagggcc actgaattca agtactagtt tagggcaaaa cacttccacc tcttcaaatt
     7861 tgcaaaggac agtggttact gtaggcaata aaaacagtca cttggatgga tcttcatctt
     7921 cagaaatgaa gcagtccagt gcttcagact tggtgtccaa gagctcctct ttaaagggag
     7981 agaagaccaa agtgctgagt tccaagagct cagagggatc tgcacataat gtggcttacc
     8041 ctggaattcc taaactggcc ccacaggttc ataacacaac atctagagaa ctgaatgtta
     8101 gtaaaatcgg ctcctttgct gaaccctctt cagtgtcgtt ttcttctaaa gaggccctct
     8161 ccttcccaca cctccatttg agagggcaaa ggaatgatcg agaccaacac acagattcta
     8221 cccaatcagc aaactcctct ccagatgaag atactgaagt caaaaccttg aagctatctg
     8281 gaatgagcaa cagatcatcc attatcaacg aacatatggg atctagttcc agagatagga
     8341 gacagaaagg gaaaaaatcc tgtaaagaaa ctttcaaaga aaagcattcc agtaaatctt
     8401 ttttggaacc tggtcaggtg acaactggtg aggaaggaaa cttgaagcca gagtttatgg
     8461 atgaggtttt gactcctgag tatatgggcc aacgaccatg taacaatgtt tcttctgata
     8521 agattggtga taaaggcctt tctatgccag gagtccccaa agctccaccc atgcaagtag
     8581 aaggatctgc caaggaatta caggcaccac ggaaacgcac agtcaaagtg acactgacac
     8641 ctctaaaaat ggaaaatgag agtcaatcca aaaatgccct gaaagaaagt agtcctgctt
     8701 cccctttgca aatagagtca acatctccca cagaaccaat ttcagcctct gaaaatccag
     8761 gagatggtcc agtggcccaa ccaagcccca ataatacctc atgccaggat tctcaaagta
     8821 acaactatca gaatcttcca gtacaggaca gaaacctaat gcttccagat ggccccaaac
     8881 ctcaggagga tggctctttt aaaaggaggt atccccgtcg cagtgcccgt gcacgttcta
     8941 acatgttttt tgggcttacc ccactctatg gagtaagatc ctatggtgaa gaagacattc
     9001 cattctacag cagctcaact gggaagaagc gaggcaagag atcagctgaa ggacaggtgg
     9061 atggggccga tgacttaagc acttcagatg aagacgactt atactattac aacttcacta
     9121 gaacagtgat ttcttcaggt ggagaggaac gactggcatc ccataattta tttcgggagg
     9181 aggaacagtg tgatcttcca aaaatctcac agttggatgg tgttgatgat gggacagaga
     9241 gtgatactag tgtcacagcc acaacaagga aaagcagcca gattccaaaa agaaatggta
     9301 aagaaaatgg aacagagaac ttaaagattg atagacctga agatgctggg gagaaagaac
     9361 atgtcactaa gagttctgtt ggccacaaaa atgagccaaa gatggataac tgccattctg
     9421 taagcagagt taaaacacag ggacaagatt ccttggaagc tcagctcagc tcattggagt
     9481 caagccgcag agtccacaca agtaccccct ccgacaaaaa tttactggac acctataata
     9541 ctgagctcct gaaatcagat tcagacaata acaacagtga tgactgtggg aatatcctgc
     9601 cttcagacat tatggacttt gtactaaaga atactccatc catgcaggct ttgggtgaga
     9661 gcccagagtc atcttcatca gaactcctga atcttggtga aggattgggt cttgacagta
     9721 atcgtgaaaa agacatgggt ctttttgaag tattttctca gcagctgcct acaacagaac
     9781 ctgtggatag tagtgtctct tcctctatct cagcagagga acagtttgag ttgcctctag
     9841 agctaccatc tgatctgtct gtcttgacca cccggagtcc cactgtcccc agccagaatc
     9901 ccagtagact agctgttatc tcagactcag gggagaagag agtaaccatc acagaaaaat
     9961 ctgtagcctc ctctgaaagt gacccagcac tgctgagccc aggagtagat ccaactcctg
    10021 aaggccacat gactcctgat cattttatcc aaggacacat ggatgcagac cacatctcta
    10081 gccctccttg tggttcagta gagcaaggtc atggcaacaa tcaggattta actaggaaca
    10141 gtagcacccc tggccttcag gtacctgttt ccccaactgt tcccatccag aaccagaagt
    10201 atgtgcccaa ttctactgat agtcctggcc cgtctcagat ttccaatgca gctgtccaga
    10261 ccactccacc ccacctgaag ccagccactg agaaactcat agttgttaac cagaacatgc
    10321 agccacttta tgttctccaa actcttccaa atggagtgac ccaaaaaatc caattgacct
    10381 cttctgttag ttctacaccc agtgtgatgg agacaaatac ttcagtattg ggacccatgg
    10441 gaggtggtct cacccttacc acaggactaa atccaagctt gccaacttct caatctttgt
    10501 tcccttctgc tagcaaagga ttgctaccca tgtctcatca ccagcactta cattccttcc
    10561 ctgcagctac tcaaagtagt ttcccaccaa acatcagcaa tcctccttca ggcctgctta
    10621 ttggggttca gcctcctccg gatccccaac ttttggtttc agaatccagc cagaggacag
    10681 acctcagtac cacagtagcc actccatcct ctggactcaa gaaaagaccc atatctcgtc
    10741 tacagacccg aaagaataaa aaacttgctc cctctagtac cccttcaaac attgcccctt
    10801 ctgatgtggt ttctaatatg acattgatta acttcacacc ctcccagctt cctaatcatc
    10861 caagtctgtt agatttgggg tcacttaata cttcatctca ccgaactgtc cccaacatca
    10921 taaaaagatc taaatctagc atcatgtatt ttgaaccggc acccctgtta ccacagagtg
    10981 tgggaggaac tgctgccaca gcggcaggca catcaacaat aagccaggat actagccacc
    11041 tcacatcagg gtctgtgtct ggcttggcat ccagttcctc tgtcttgaat gttgtatcca
    11101 tgcaaactac cacaacccct acaagtagtg cgtcagttcc aggacacgtc accttaacca
    11161 acccaaggtt gcttggtacc ccagatattg gctcaataag caatctttta atcaaagcta
    11221 gccagcagag cctggggatt caggaccagc ctgtggcttt accgccaagt tcaggaatgt
    11281 ttccacaact ggggacatca cagaccccct ctactgctgc aataacagcg gcatctagca
    11341 tctgtgtgct cccctccact cagactacgg gcataacagc cgcttcacct tctggggaag
    11401 cagacgaaca ctatcagctt cagcatgtga accagctcct tgccagcaaa actgggattc
    11461 attcttccca gcgtgatctt gattctgctt cagggcccca ggtatccaac tttacccaga
    11521 cggtagacgc tcctaatagc atgggactgg agcagaacaa ggctttatcc tcagctgtgc
    11581 aagccagccc cacctctcct gggggttctc catcctctcc atcttctgga cagcggtcag
    11641 caagcccttc agtgccgggt cccactaaac ccaaaccaaa aaccaaacgg tttcagctgc
    11701 ctctagacaa agggaatggc aagaagcaca aagtttccca tttgcggacc agttcttctg
    11761 aagcacacat tccagaccaa gaaacgacat ccctgacctc aggcacaggg actccaggag
    11821 cagaggctga gcagcaggat acagctagcg tggagcagtc ctcccagaag gagtgtgggc
    11881 aacctgcagg gcaagtcgct gttcttccgg aagttcaggt gacccaaaat ccagcaaatg
    11941 aacaagaaag tgcagaacct aaaacagtgg aagaagagga aagtaatttc agctccccac
    12001 tgatgctttg gcttcagcaa gaacaaaagc ggaaggaaag cattactgag aaaaaaccca
    12061 agaaaggact tgtttttgaa atttccagtg atgatggctt tcagatctgt gcagaaagta
    12121 ttgaagatgc ctggaagtca ttgacagata aagtccagga agctcgatca aatgcccgcc
    12181 taaagcagct ctcatttgca ggtgttaacg gtttgaggat gctggggatt ctccatgatg
    12241 cagttgtgtt cctcattgag cagctgtctg gtgccaagca ctgtcgaaat tacaaattcc
    12301 gtttccacaa gccagaggag gccaatgaac cccccttgaa ccctcacggc tcagccaggg
    12361 ctgaagtcca cctcaggaag tcagcatttg acatgtttaa cttcctggct tctaaacatc
    12421 gtcagcctcc tgaatacaac cccaatgatg aagaagagga ggaggtacag ctgaagtcag
    12481 ctcggagggc aactagcatg gatctgccaa tgcccatgcg cttccggcac ttaaaaaaga
    12541 cttctaagga ggcagttggt gtctacaggt ctcccatcca tggccggggt cttttctgta
    12601 agagaaacat tgatgcaggt gagatggtga ttgagtatgc cggcaacgtc atccgctcca
    12661 tccagactga caagcgggaa aagtattacg acagcaaggg cattggttgc tatatgttcc
    12721 gaattgatga ctcagaggta gtggatgcca ccatgcatgg aaatgctgca cgcttcatca
    12781 atcactcgtg tgagcctaac tgctattctc gggtcatcaa tattgatggg cagaagcaca
    12841 ttgtcatctt tgccatgcgt aagatctacc gaggagagga actcacttac gactataagt
    12901 tccccattga ggatgccagc aacaagctgc cctgcaactg tggcgccaag aaatgccgga
    12961 agttcctaaa cgtttctctc ggctcgagcg gcgtcttcac actcgaagat ttcgttgggg
    13021 actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga ggtgtgtcca
    13081 gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg
    13141 aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg agcggcgacc
    13201 aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat catcacttta
    13261 aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac atgatcgact
    13321 atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc actgtaacag
    13381 ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc gacggctccc
    13441 tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa cgcattctgg
    13501 cgtaaggccg cgactctaga gtcgacctgc aggcatgcaa gctgatccgg ctgctaacaa
    13561 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
    13621 tggggcggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
    13681 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
    13741 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
    13801 aggttcaggg ggagatgtgg gaggtttttt taagcaagta aaacctctac aaatgtggta
    13861 aaatcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
    13921 cgcatctgtg cggtatttca caccgcatac gcggatctgc gcagcaccat ggcctgaaat
    13981 aacctctgaa agaggaactt ggttaggtac cttctgaggc ggaaagaacc agctgtggaa
    14041 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    14101 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    14161 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    14221 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    14281 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
    14341 aggctttttt ggaggcctag gcttttgcaa aaagcttaat taactgttga caattaatca
    14401 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac ccaggaggca
    14461 gatcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
    14521 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
    14581 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
    14641 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
    14701 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
    14761 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
    14821 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
    14881 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
    14941 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
    15001 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
    15061 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
    15121 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
    15181 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
    15241 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
    15301 aacctgccat cacgatggcc gcaataaaat atctttattt tcattacatc tgtgtgttgg
    15361 ttttttgtgt gaatcgatag cgataaggat cctctttgcg cttgcgtttt cccttgtcca
    15421 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
    15481 cccggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    15541 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    15601 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    15661 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    15721 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    15781 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    15841 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    15901 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    15961 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    16021 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    16081 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    16141 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatttcaaga
    16201 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    16261 gattttggtc atgagattat caaaaaggat cttcacctag atccttttat agtccggaaa
    16321 tacaggaacg cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc
    16381 ttcagtggat taagtggggg taatgtggcc tgtaccctct ggttgcatag gtattcatac
    16441 ggttaaaatt tatcaggcgc gattgcggca gtttttcggg tggtttgttg ccatttttac
    16501 ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt aagggattat
    16561 ggtaaatcca cttactgtct gccctcgtag ccatcgagat aaaccgcagt actccggcca
    16621 cgatgcgtcc ggcgtagagg atcgagatct
//
