LOCUS       sequence-473369-        6365 bp    DNA     circular SYN 14-JUL-2025
DEFINITION  Express ADARB1-HiBiT in Mammalian Cells under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6365)
  TITLE     Promega Early Access Collection
REFERENCE   2  (bases 1 to 6365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ADARB1-HiBiT Fusion Vector
FEATURES             Location/Qualifiers
     source          1..6365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3444..3462)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    3499..3620
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3510..3529)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3564..3583
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3865..4222
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3865..4056
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3888..3908)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4073..4208
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4237..4284
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4316..5110
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4370..4389)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4980..4999
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5174..5222
                     /note="synthetic polyadenylation signal"
     rep_origin      5379..5924
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     5825..5844
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6041..6058
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggat atagaagatg
     1081 aagaaaacat gagttccagc agcactgatg tgaaggaaaa ccgcaatctg gacaacgtgt
     1141 cccccaagga tggcagcaca cctgggcctg gcgagggctc tcagctctcc aatgggggtg
     1201 gtggtggccc cggcagaaag cggcccctgg aggagggcag caatggccac tccaagtacc
     1261 gcctgaagaa aaggaggaaa acaccagggc ccgtcctccc caagaacgcc ctgatgcagc
     1321 tgaatgagat caagcctggt ttgcagtaca cactcctgtc ccagactggg cccgtgcacg
     1381 cgcctttgtt tgtcatgtct gtggaggtga atggccaggt ttttgagggc tctggtccca
     1441 caaagaaaaa ggcaaaactc catgctgctg agaaggcctt gaggtctttc gttcagtttc
     1501 ctaatgcctc tgaggcccac ctggccatgg ggaggaccct gtctgtcaac acggacttca
     1561 catctgacca ggccgacttc cctgacacgc tcttcaatgg ttttgaaact cctgacaagg
     1621 cggagcctcc cttttacgtg ggctccaatg gggatgactc cttcagttcc agcggggacc
     1681 tcagcttgtc tgcttccccg gtgcctgcca gcctagccca gcctcctctc cctgtcttac
     1741 caccattccc acccccgagt gggaagaatc ccgtgatgat cttgaacgaa ctgcgcccag
     1801 gactcaagta tgacttcctc tccgagagcg gggagagcca tgccaagagc ttcgtcatgt
     1861 ctgtggtcgt ggatggtcag ttctttgaag gctcggggag aaacaagaag cttgccaagg
     1921 cccgggctgc gcagtctgcc ctggccgcca tttttaactt gcacttggat cagacgccat
     1981 ctcgccagcc tattcccagt gagggtcttc agctgcattt accgcaggtt ttagctgacg
     2041 ctgtctcacg cctggtcctg ggtaagtttg gtgacctgac cgacaacttc tcctcccctc
     2101 acgctcgcag aaaagtgctg gctggagtcg tcatgacaac aggcacagat gttaaagatg
     2161 ccaaggtgat aagtgtttct acaggaacaa aatgtattaa tggtgaatac atgagtgatc
     2221 gtggccttgc attaaatgac tgccatgcag aaataatatc tcggagatcc ttgctcagat
     2281 ttctttatac acaacttgag ctttacttaa ataacaaaga tgatcaaaaa agatccatct
     2341 ttcagaaatc agagcgaggg gggtttaggc tgaaggagaa tgtccagttt catctgtaca
     2401 tcagcacctc tccctgtgga gatgccagaa tcttctcacc acatgagcca atcctggaag
     2461 ggtctcgctc ttacacccag gctggagtgc agtggtgcaa tcatggctca ctgcagcctc
     2521 gacctcctgg gctcttaagc gatccttcca cctcaacctt ccaaggagct gggactacag
     2581 aaccagcaga tagacaccca aatcgtaaag caagaggaca gctacggacc aaaatagagt
     2641 ctggtgaggg gacgattcca gtgcgctcca atgcgagcat ccaaacgtgg gacggggtgc
     2701 tgcaagggga gcggctgctc accatgtcct gcagtgacaa gattgcacgc tggaacgtgg
     2761 tgggcatcca gggatccctg ctcagcattt tcgtggagcc catttacttc tcgagcatca
     2821 tcctgggcag cctttaccac ggggaccacc tttccagggc catgtaccag cggatctcca
     2881 acatagagga cctgccacct ctctacaccc tcaacaagcc tttgctcagt ggcatcagca
     2941 atgcagaagc acggcagcca gggaaggccc ccaacttcag tgtcaactgg acggtaggcg
     3001 actccgctat tgaggtcatc aacgccacga ctgggaagga tgagctgggc cgcgcgtccc
     3061 gcctgtgtaa gcacgcgttg tactgtcgct ggatgcgtgt gcacggcaag gttccctccc
     3121 acttactacg ctccaagatt accaagccca acgtgtacca tgagtccaag ctggcggcaa
     3181 aggagtacca ggccgccaag gcgcgtctgt tcacagcctt catcaaggcg gggctggggg
     3241 cctgggtgga gaagcccacc gagcaggacc agttctcact cacgcccgtt tctcaaggca
     3301 gttcaggtgg ctcgagcggt gtgagcggct ggcggctgtt caagaagatt agctaaggcc
     3361 gcgactctag agtcgacctg caggcatgca agctgatccg gctgctaaca aagcccgaaa
     3421 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcggc
     3481 cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     3541 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     3601 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     3661 gggagatgtg ggaggttttt ttaagcaagt aaaacctcta caaatgtggt aaaatcgaat
     3721 tttaacaaaa tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     3781 gcggtatttc acaccgcata cgcggatctg cgcagcacca tggcctgaaa taacctctga
     3841 aagaggaact tggttaggta ccttctgagg cggaaagaac cagctgtgga atgtgtgtca
     3901 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     3961 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     4021 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4081 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4141 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     4201 tggaggccta ggcttttgca aaaagcttaa ttaactgttg acaattaatc atcggcatag
     4261 tatatcggca tagtataata cgacaaggtg aggaactaaa cccaggaggc agatcatgat
     4321 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     4381 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     4441 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     4501 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     4561 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     4621 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     4681 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     4741 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     4801 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     4861 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     4921 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     4981 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     5041 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     5101 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     5161 tcacgatggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg gttttttgtg
     5221 tgaatcgata gcgataagga tccgctcatg cgatgataag ctgtcaaaca tgagaattac
     5281 aacttatatc gtatggggct gacttcaggt gctacatttg aagagataaa ttgcactgaa
     5341 atctagaaat attttatctg attaataaga tgatcttctt gagatcgttt tggtctgcgc
     5401 gtaatctctt gctctgaaaa cgaaaaaacc gccttgcagg gcggtttttc gaaggttctc
     5461 tgagctacca actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaaactt
     5521 gtcctttcag tttagcctta accggcgcat gacttcaaga ctaactcctc taaatcaatt
     5581 accagtggct gctgccagtg gtgcttttgc atgtctttcc gggttggact caagacgata
     5641 gttaccggat aaggcgcagc ggtcggactg aacggggggt tcgtgcatac agtccagctt
     5701 ggagcgaact gcctacccgg aactgagtgt caggcgtgga atgagacaaa cgcggccata
     5761 acagcggaat gacaccggta aaccgaaagg caggaacagg agagcgcacg agggagccgc
     5821 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc
     5881 gtcagatttc gtgatgcttg tcaggggggc ggagcctatg gaaaaacggc tttgccgcgg
     5941 ccctctcact tccctgttaa gtatcttcct ggcatcttcc aggaaatctc cgccccgttc
     6001 gtaagccatt tccgctcgcc gcagtcgaac gaccgagcgt agcgagtcag tgagcgagga
     6061 agcggaatat atcctgtatc acatattctg ctgacgcacc ggtgcagcct tttttctcct
     6121 gccacatgaa gcacttcact gacaccctca tcagtgccaa catagtaagc cagtatacac
     6181 tccgctagcg ctgatgtccg gcggtgcttt tgccgttacg caccaccccg tcatgagatt
     6241 acgcgtttta gcggtgcgta caattaaggg attatggtaa atccacttac tgtctgccct
     6301 cgtagccatc gagataaacc gcagtactcc ggccacgatg cgtccggcgt agaggatcga
     6361 gatct
//
