LOCUS       sequence-47372-e        4683 bp    DNA     circular SYN 15-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Markus SM, Plevock KM, St Germain BJ, Punch JJ, Meaden CW, Lee WL
  TITLE     Quantitative analysis of Pac1/LIS1-mediated dynein targeting: 
            Implications for regulation of dynein activity in budding yeast.
  JOURNAL   Cytoskeleton (Hoboken). 2011 Mar;68(3):157-74. doi: 
            10.1002/cm.20502. Epub 2011 Feb 3.
  PUBMED    21294277
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/cm.20502"; 
            journalName: "Cytoskeleton (Hoboken)"; date: "2011-03"; volume: 
            "68"; issue: "3"; pages: "157-74"
COMMENT     Sequence Label: pRS303:VC
FEATURES             Location/Qualifiers
     source          1..4683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        86..190
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(191..259,260..1051)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(409..428)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1222..1810
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1964..1981
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(2134..2139,2140..2157,2158..2164)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2172..2188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2177..2199
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2196..2212
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2196..2212
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        2233..2251
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2619..2638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2620..2638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2645..2662)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2645..2661)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2654..2676)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2806..3261
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2893..2912)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3103..3124
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(3522..4181)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(4182..4369)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     primer_bind     complement(4514..4533)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4633..4655
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataagacgtc
       61 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      121 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      181 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      241 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      301 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      361 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      421 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      481 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      541 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      601 gacaacgatc ggaggaccga aggagctaac cgcttttttt cacaacatgg gggatcatgt
      661 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      721 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      781 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      841 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      901 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      961 agttatctac acgacgggca gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     1021 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1081 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1141 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1201 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1261 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1321 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     1381 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1441 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1501 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1561 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga
     1621 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1681 aacaggagag cgcacgaggg agcttccagg ggggaacgcc tggtatcttt atagtcctgt
     1741 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggccgag
     1801 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1861 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1921 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1981 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2041 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2101 tgtgagttac ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcctat
     2161 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2221 cgccaagctc ggaattaacc ctcactaaag ggaacaaaag ctgggtaccg ggccccccct
     2281 cgaggcttgt aaaattccaa atgaattaaa aggtaaagtt atgaatcata tggacaaaca
     2341 aaagaatggt atcaaagcta acttcaaaat tagacacaac attgaagatg gtggtgttca
     2401 attagctgac cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga
     2461 caaccattac ttatcctatc aatctgcctt atccaaagat ccaaacgaaa agagagacca
     2521 catggtcttg ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta
     2581 caaataagcg gccgccaccg cggtggagct ccaattcgcc ctatagtgag tcgtattaca
     2641 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     2701 atcgccttgc agcacatccc cccttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     2761 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcgacgcg ccctgtagcg
     2821 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2881 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2941 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3001 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3061 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3121 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3181 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     3241 aaatattaac gtttacaatt tcctgatgcg gtattttctc cttacgcatc tgtgcggtat
     3301 ttcacaccgc atatgatccg tcgagttcaa gagaaaaaaa aagaaaaagc aaaaagaaaa
     3361 aaggaaagcg cgcctcgttc agaatgacac gtatagaatg atgcattacc ttgtcatctt
     3421 cagtatcata ctgttcgtat acatacttac tgacattcat aggtatacat atatacacat
     3481 gtatatatat cgtatgctgc agctttaaat aatcggtgtc actacataag aacacctttg
     3541 gtggagggaa catcgttggt accattgggc gaggtggctt ctcttatggc aaccgcaaga
     3601 gccttgaacg cactctcact acggtgatga tcattcttgc ctcgcagaca atcaacgtgg
     3661 agggtaattc tgctagcctc tgcaaagctt tcaagaaaat gcgggatcat ctcgcaagag
     3721 agatctccta ctttctccct ttgcaaacca agttcgacaa ctgcgtacgg cctgttcgaa
     3781 agatctacca ccgctctgga aagtgcctca tccaaaggcg caaatcctga tccaaacctt
     3841 tttactccac gcgccagtag ggcctcttta aaagcttgac cgagagcaat cccgcagtct
     3901 tcagtggtgt gatggtcgtc tatgtgtaag tcaccaatgc actcaacgat tagcgaccag
     3961 ccggaatgct tggccagagc atgtatcata tggtccagaa accctatacc tgtgtggacg
     4021 ttaatcactt gcgattgtgt ggcctgttct gctactgctt ctgcctcttt ttctgggaag
     4081 atcgagtgct ctatcgctag gggaccaccc tttaaagaga tcgcaatctg aatcttggtt
     4141 tcatttgtaa tacgctttac tagggctttc tgctctgtca tctttgcctt cgtttatctt
     4201 gcctgctcat tttttagtat attcttcgaa gaaatcacat tactttatat aatgtataat
     4261 tcattatgtg ataatgccaa tcgctaagaa aaaaaaagag tcatccgcta ggtggaaaaa
     4321 aaaaaatgaa aatcattacc gaggcataaa aaaatataga gtgtactaga ggaggccaag
     4381 agtaatagaa aaagaaaatt gcgggaaagg actgtgttat gacttccctg actaatgccg
     4441 tgttcaaacg atacctggca gtgactccta gcgctcacca agctcttaaa acggaattat
     4501 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     4561 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     4621 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     4681 cga
//
