LOCUS       sequence-473911-        9453 bp    DNA     circular SYN 28-MAY-2025
DEFINITION  A synthetic transcription factor (synTF) that contains concensus 
            3CLpro cleavage site (CS).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9453)
  TITLE     Optimized Pipeline and Designer Cells for Synthetic Biology Based 
            High-Throughput Screening of Viral Protease Inhibitors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTE3: pFuGW-SSX1p-GAL4BD-1X3CLpro consensus
            CS...
FEATURES             Location/Qualifiers
     source          1..9453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..218
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     203..219
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    222..810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             328..339
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     737..757
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             1675..2115
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPMCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2290..2310
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2398..2515
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2813..2857)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3042..3275
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3768..3893
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3940..4120
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4138..4336)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4163..4183)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4338..4717
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4889..4908
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4983..5087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5088..5156,5157..5948)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5306..5325)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6119..6707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6608..6627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6861..6878
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6995..7016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7031..7036,7037..7054,7055..7061)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7069..7085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7074..7096
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7093..7109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7093..7109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7158..7279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7169..7188)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7223..7242
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7409..7783)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7802..7849)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7897..8226)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7911..8046
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7965..7984)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8211..8231
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8240..8668)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8350..8371)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8562..8581
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8714..8938
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8927..8944
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8970..9150
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
       61 cttgtaagtc attggtctta aaggtacctg aggtgtgact ggaaaaccca cctcctcctc
      121 ctcttgtgct tctagccagg cacaatcagc attggtagct gctgtattgc tacttgtgat
      181 tgctccatgt ttttctaggt ctcgaggtcg acggtatcga tgcggggagg cggcccaaag
      241 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
      301 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
      361 cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc
      421 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
      481 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
      541 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
      601 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
      661 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
      721 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
      781 ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcgg cggccgccct
      841 gcagggtagc cagatcatgg ctcactgcaa cctcgtactc ctgggctcaa gctatcctcc
      901 tacctcagcc tcctgagtaa cggactacag gcacaccacc ccacctcgct aattttattt
      961 attttttttg tagagaaaag agacagggta ttgctctgtt gcccagggtg gggtgcagtg
     1021 gcatgatcat ggctcactgc aacctctgcc tcccaggttc aagtgatcct ccagctgtgg
     1081 cctccctaag tgctgggatt acaaccgtga gccgccgcac cggcccaaat ttcttacgtc
     1141 actacagagt tcctaggaaa aaatcccata cctgaaaaag atagaaactg acaggaagga
     1201 tttgagatga tgacctgctt catatacact ccttattaaa actggataac aatgcaccac
     1261 cgaggaggtg gggagggata ggaaaaatga aaagagaaaa tcagcgcatg cgtactctga
     1321 tttgggaaga ctccaaagag aaaatcagag catgcgtact ctgaacttgg agtagccaat
     1381 cccaggggat gctttaggcg ggaaagtcag agtttctgcc tccattttga gaaggttctg
     1441 tccctagagc ctagactgat agaccccaca tcagcttggc ttgtcccgcc tactgttctg
     1501 acttctgatt ggccagatgg agttcactaa ctgccctgat tggtccatca tcctggagca
     1561 atgacattgc agaatatttt ctcctcctcc agccacactt tgtcaccaac tgctgccaac
     1621 tcgccaccac tgctgccgac ctcgcaacca ctgctttgtc tctggcgcgc catgatgaag
     1681 ctactgtctt ctatcgaaca agcatgcgat atttgccgac ttaaaaagct caagtgctcc
     1741 aaagaaaaac cgatgtgcgc caagtgtctg aagaacaact gggagtgtcg ctactctccc
     1801 aaaaccaaaa ggtctccgct gactagggca catctgacag aagtggaatc aaggctagaa
     1861 agactggaac agctatttct actgattttt cctcgagaag accttgacat gattttgaaa
     1921 atggattctt tacaggatat aaaagcattg ttaacaggat tatttgtaca agataatgtg
     1981 aataaagatg ccgtcacaga tagattggct tcagtggaga ctgatatgcc tctaacattg
     2041 agacagcata gaataagtgc gacatcatca tcggaagaga gtagtaacaa aggtcaaaga
     2101 cagttgactg tatcggaatt cgctgtactt caatctggct tcagaaccgg ttcgacggcc
     2161 cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg
     2221 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg
     2281 ggtccgggac caaagaagaa gcggaaggtc tagggatcct tgcagtcccg ggtaagcggg
     2341 actctggggt tcgaaaatta accaaactgg atctctgctg tccctgtaat aaacccgaaa
     2401 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     2461 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     2521 gatgaatact gccatttgtc tgcagaattg gcgcacgcag tgccgatccg ttcactaatc
     2581 gaatggatct gtctctgtct ctctctccac cttcttcttc tattccttcg ggcctgtcgg
     2641 gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc
     2701 tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca
     2761 ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg
     2821 cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt
     2881 caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg
     2941 tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag
     3001 cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta
     3061 ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt
     3121 gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac
     3181 cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc
     3241 ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct
     3301 gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt
     3361 tatatttata taattcactt ctccaattgt ccctcatatc tcctcctcca ggtctgaaga
     3421 tcagcggccg cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct
     3481 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     3541 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     3601 agtatttgtc tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct
     3661 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     3721 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     3781 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     3841 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     3901 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     3961 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     4021 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     4081 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaacgcgctg
     4141 cttatataga cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg
     4201 ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca
     4261 atggggtgga gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac
     4321 tgccaaaacc gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     4381 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     4441 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     4501 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     4561 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     4621 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     4681 attgattact attaataact agtcaataat caatgtcaac gcgtatatct ggcccgtaca
     4741 tcgcgaagca gcgcaaaacg cctaacccta agcagattct tcatgcaatt gtcggtcaag
     4801 ccttgccttg ttgtagctta aattttgctc gcgcactact cagcgacctc caacacacaa
     4861 gcagggagca gatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
     4921 cataggggat cgggagatct cccgatccgt cgacgtcagg tggcactttt cggggaaatg
     4981 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     5041 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     5101 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     5161 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     5221 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     5281 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     5341 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     5401 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     5461 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     5521 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     5581 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     5641 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     5701 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     5761 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     5821 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     5881 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     5941 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     6001 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     6061 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     6121 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6181 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6241 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     6301 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6361 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6421 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6481 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6541 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6601 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6661 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6721 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6781 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6841 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     6901 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     6961 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     7021 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     7081 aacaatttca cacaggaaac agctatgacc atgattacgc caagctctag ctagaggtcg
     7141 acggtataca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     7201 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     7261 aagctgcaat aaacaagttg gggtgggcga agaactccag catgagatcc ccgcgctgga
     7321 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
     7381 cggtggaatc gaaatctcgt agcacgtgtc agtcctgctc ctcggccacg aagtgcacgc
     7441 agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg ccgatctcgg
     7501 tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac cactcggcgt
     7561 acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc accacctggt
     7621 cctggaccgc gctgatgaac agggtcacgt cgtcccggac cacaccggcg aagtcgtcct
     7681 ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct ccggcgacgt
     7741 cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggtttag ttcctcacct
     7801 tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca cgtgctgatc
     7861 agatccgaaa atggatatac aagctcccgg gagctttttg caaaagccta ggcctccaaa
     7921 aaagcctcct cactacttct ggaatagctc agaggcagag gcggcctcgg cctctgcata
     7981 aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg
     8041 cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg
     8101 catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag
     8161 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg
     8221 acacacattc cacagaatta attcgcgtta aatttttgtt aaatcagctc attttttaac
     8281 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     8341 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     8401 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     8461 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     8521 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     8581 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     8641 gcgcttaatg cgccgctaca gggcgcgtgg ggataccccc tagagcccca gctggttctt
     8701 tccgcctcag aagccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
     8761 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
     8821 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
     8881 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagtc
     8941 gaggctgatc agcgggttta aacgggccct gctagagatt ttccacactg actaaaaggg
     9001 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     9061 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     9121 ggctcagatc tggtctaacc agagagaccc agtacagtcc ggatgcagct ctcgggccat
     9181 gtgatgaaat gctaggcggc tgtcaaacct ccactctaat acttctctct ccgggtcatc
     9241 catcccatgc aggctcacag ggtgtaacaa gcgggtgttc tctccttcat tggcttcttc
     9301 taccttctct tgctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
     9361 gatccctggc cctggtgtgt agttctgcca atcagggaag tagccttgtg tgtggtagat
     9421 ccacagatca aggatatctt gtcttcgttg gga
//
