LOCUS       sequence-473919-        9546 bp    DNA     circular SYN 28-MAY-2025
DEFINITION  a synthetic transcription factor (synTF) that contains 4 tandem 
            synthetic general 3CLpro cleavage site (CS)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9546)
  TITLE     Optimized Pipeline and Designer Cells for Synthetic Biology Based 
            High-Throughput Screening of Viral Protease Inhibitors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9546)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTE5: pFuGW-SSX1p- GAL4BD-4X3CLpro synthetic g...
FEATURES             Location/Qualifiers
     source          1..9546
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     202..218
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     203..219
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    222..810
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             328..339
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     737..757
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             1675..2115
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             2383..2403
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2491..2608
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2906..2950)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3135..3368
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3861..3986
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4033..4213
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4231..4429)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4256..4276)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4431..4810
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4982..5001
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5076..5180
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5181..5249,5250..6041)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5399..5418)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6212..6800
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6701..6720
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6954..6971
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7088..7109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7124..7129,7130..7147,7148..7154)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7162..7178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7167..7189
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7186..7202
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7186..7202
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    7251..7372
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7262..7281)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7316..7335
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(7502..7876)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7895..7942)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7990..8319)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8004..8139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8058..8077)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8304..8324
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(8333..8761)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8443..8464)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8655..8674
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    8807..9031
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9020..9037
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             9063..9243
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gtgaattagc ccttccagtc cccccttttc ttttaaaaag tggctaagat ctacagctgc
       61 cttgtaagtc attggtctta aaggtacctg aggtgtgact ggaaaaccca cctcctcctc
      121 ctcttgtgct tctagccagg cacaatcagc attggtagct gctgtattgc tacttgtgat
      181 tgctccatgt ttttctaggt ctcgaggtcg acggtatcga tgcggggagg cggcccaaag
      241 ggagatccga ctcgtctgag ggcgaaggcg aagacgcgga agaggccgca gagccggcag
      301 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
      361 cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc
      421 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
      481 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
      541 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
      601 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
      661 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
      721 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
      781 ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcgg cggccgccct
      841 gcagggtagc cagatcatgg ctcactgcaa cctcgtactc ctgggctcaa gctatcctcc
      901 tacctcagcc tcctgagtaa cggactacag gcacaccacc ccacctcgct aattttattt
      961 attttttttg tagagaaaag agacagggta ttgctctgtt gcccagggtg gggtgcagtg
     1021 gcatgatcat ggctcactgc aacctctgcc tcccaggttc aagtgatcct ccagctgtgg
     1081 cctccctaag tgctgggatt acaaccgtga gccgccgcac cggcccaaat ttcttacgtc
     1141 actacagagt tcctaggaaa aaatcccata cctgaaaaag atagaaactg acaggaagga
     1201 tttgagatga tgacctgctt catatacact ccttattaaa actggataac aatgcaccac
     1261 cgaggaggtg gggagggata ggaaaaatga aaagagaaaa tcagcgcatg cgtactctga
     1321 tttgggaaga ctccaaagag aaaatcagag catgcgtact ctgaacttgg agtagccaat
     1381 cccaggggat gctttaggcg ggaaagtcag agtttctgcc tccattttga gaaggttctg
     1441 tccctagagc ctagactgat agaccccaca tcagcttggc ttgtcccgcc tactgttctg
     1501 acttctgatt ggccagatgg agttcactaa ctgccctgat tggtccatca tcctggagca
     1561 atgacattgc agaatatttt ctcctcctcc agccacactt tgtcaccaac tgctgccaac
     1621 tcgccaccac tgctgccgac ctcgcaacca ctgctttgtc tctggcgcgc catgatgaag
     1681 ctactgtctt ctatcgaaca agcatgcgat atttgccgac ttaaaaagct caagtgctcc
     1741 aaagaaaaac cgaagtgcgc caagtgtctg aagaacaact gggagtgtcg ctactctccc
     1801 aaaaccaaaa ggtctccgct gactagggca catctgacag aagtggaatc aaggctagaa
     1861 agactggaac agctatttct actgattttt cctcgagaag accttgacat gattttgaaa
     1921 atggattctt tacaggatat aaaagcattg ttaacaggat tatttgtaca agataatgtg
     1981 aataaagatg ccgtcacaga tagattggct tcagtggaga ctgatatgcc tctaacattg
     2041 agacagcata gaataagtgc gacatcatca tcggaagaga gtagtaacaa aggtcaaaga
     2101 cagttgactg tatcggaatt cgtagcccgc ttgcaaagcg ggttcaccgg tgtagcccgc
     2161 ttgcaaagcg ggttcaccgg tgtagctcga ttgcagagtg gattcaccgg tgtagcaagg
     2221 cttcaatcag gattcttaat taattcgacg gcccccccga ccgatgtcag cctgggggac
     2281 gagctccact tagacggcga ggacgtggcg atggcgcatg ccgacgcgct agacgatttc
     2341 gatctggaca tgttggggga cggggattcc ccgggtccgg gaccaaagaa gaagcggaag
     2401 gtctagggat ccttgcagtc ccgggtaagc gggactctgg ggttcgaaaa ttaaccaaac
     2461 tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa tttgtttttg
     2521 taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc ctgcactgta
     2581 ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt gtctgcagaa
     2641 ttggcgcacg cagtgccgat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     2701 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     2761 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     2821 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     2881 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     2941 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     3001 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     3061 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     3121 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     3181 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     3241 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     3301 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     3361 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     3421 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     3481 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg tgcggtggtc
     3541 ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc ttttatctct
     3601 atcctttgat gcacacaata gagggttgct actgtattat ataatgatct aagttcttct
     3661 gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc ttctgatgtt
     3721 tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca tactatatgt
     3781 tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca tcgcgatcta
     3841 attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta gcctccgcta
     3901 gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca
     3961 gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt caagtccctg
     4021 ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     4081 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     4141 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     4201 accagagaga cccagtacag gcaaaacgcg ctgcttatat agacctccca ccgtacacgc
     4261 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
     4321 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
     4381 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
     4441 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
     4501 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
     4561 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
     4621 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
     4681 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac
     4741 tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata actagtcaat
     4801 aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa acgcctaacc
     4861 ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc ttaaattttg
     4921 ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg gcttaactat
     4981 gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga tctcccgatc
     5041 cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5101 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5161 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5221 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5281 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5341 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5401 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5461 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5521 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5581 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5641 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5701 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5761 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5821 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5881 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     5941 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6001 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6061 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6121 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6841 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6901 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6961 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7021 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7081 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7141 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7201 accatgatta cgccaagctc tagctagagg tcgacggtat acagacatga taagatacat
     7261 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     7321 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttggggtggg
     7381 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
     7441 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtagcacgt
     7501 gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg cgcagggcga
     7561 actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag gcgtcccgga
     7621 agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg cgcacccaca
     7681 cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg aacagggtca
     7741 cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag aacccgagcc
     7801 ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc ggaacggcac
     7861 tggtcaactt ggccatggtt tagttcctca ccttgtcgta ttatactatg ccgatatact
     7921 atgccgatga ttaattgtca acacgtgctg atcagatccg aaaatggata tacaagctcc
     7981 cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     8041 ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     8101 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     8161 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     8221 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     8281 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa ttaattcgcg
     8341 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     8401 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     8461 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     8521 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
     8581 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     8641 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     8701 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     8761 tggggatacc ccctagagcc ccagctggtt ctttccgcct cagaagccat agagcccacc
     8821 gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc
     8881 ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat
     8941 taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa
     9001 acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc
     9061 cctgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc
     9121 acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag
     9181 cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga
     9241 cccagtacag tccggatgca gctctcgggc catgtgatga aatgctaggc ggctgtcaaa
     9301 cctccactct aatacttctc tctccgggtc atccatccca tgcaggctca cagggtgtaa
     9361 caagcgggtg ttctctcctt cattggcttc ttctaccttc tcttgctcaa ctggtactag
     9421 cttgtagcac catccaaagg tcagtggata tctgatccct ggccctggtg tgtagttctg
     9481 ccaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat cttgtcttcg
     9541 ttggga
//
