LOCUS       sequence-473921-        8858 bp    DNA     circular SYN 28-MAY-2025
DEFINITION  Synthetic GAL4p comprising 5 tandem GAL4 binding sites (G5p) 
            regulating EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8858)
  TITLE     Optimized Pipeline and Designer Cells for Synthetic Biology Based 
            High-Throughput Screening of Viral Protease Inhibitors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSE89: pFuGW-G5p-EYFP
FEATURES             Location/Qualifiers
     source          1..8858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             28..39
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     437..457
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             757..1461
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGDVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEIAGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLTE
                     TVRAVDCRKTYL"
     misc_feature    1503..1620
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(1918..1962)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2147..2380
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2873..2998
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3045..3225
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3243..3441)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3268..3288)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3443..3822
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     3994..4013
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4088..4192
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4193..4261,4262..5053)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4411..4430)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5224..5812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5713..5732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5966..5983
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6100..6121
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6136..6141,6142..6159,6160..6166)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6174..6190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6179..6201
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6198..6214
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6198..6214
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6263..6384
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6274..6293)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6328..6347
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6514..6888)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(6907..6954)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7002..7331)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7016..7151
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7070..7089)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7316..7336
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7345..7773)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7455..7476)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7667..7686
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7819..8043
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8032..8049
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8075..8255
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     8760..8776
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8761..8777
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(8780..8858,1..510)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
ORIGIN
        1 caggccgcgg gaaggaaggt ccgctggatt gagggccgaa gggacgtagc agaaggacgt
       61 cccgcgcaga atccaggtgg caacacaggc gagcagccaa ggaaaggacg atgatttccc
      121 cgacaacacc acggaattgt cagtgcccaa cagccgagcc cctgtccagc agcgggcaag
      181 gcaggcggcg atgagttccg ccgtggcaat agggaggggg aaagcgaaag tcccggaaag
      241 gagctgacag gtggtggcaa tgccccaacc agtgggggtt gcgtcagcaa acacagtgca
      301 caccacgcca cgttgcctga caacgggcca caactcctca taaagagaca gcaaccagga
      361 tttatacaag gaggagaaaa tgaaagccat acgggaagca atagcatgat acaaaggcat
      421 taaagcagcg tatccacata gcgtaaaagg agcaacatag ttaagaatac cagtcaatct
      481 ttcacaaatt ttgtaatcca gaggttgatt atcgataagc ttgatatcga taggtaccga
      541 gtttctagac ggagtactgt cctccgagcg gagtactgtc ctccgactcg agcggagtac
      601 tgtcctccga tcggagtact gtcctccgcg aattccggag tactgtcctc cgaagacgct
      661 agcggggggc tataaaaggg ggtgggggcg ttcgtcctca ctctagatct gcgatctaag
      721 taagcttggc attccggtac tgttggtaaa gccaccatga gcagcggcgc cctgctgttc
      781 cacggcaaga tcccctacgt ggtggagatg gagggcgatg tggatggcca caccttcagc
      841 atccgcggta agggctacgg cgatgccagc gtgggcaagg tggatgccca gttcatctgc
      901 accaccggcg atgtgcccgt gccctggagc accctggtga ccaccctgac ctacggcgcc
      961 cagtgcttcg ccaagtacgg ccccgagctg aaggatttct acaagagctg catgcccgat
     1021 ggctacgtgc aggagcgcac catcaccttc gagggcgatg gcaatttcaa gacccgcgcc
     1081 gaggtgacct tcgagaatgg cagcgtgtac aatcgcgtga agctgaatgg ccagggcttc
     1141 aagaaggatg gccacgtgct gggcaagaat ctggagttca atttcacccc ccactgcctg
     1201 tacatctggg gcgatcaggc caatcacggc ctgaagagcg ccttcaagat ctgccacgag
     1261 atcgccggca gcaagggcga tttcatcgtg gccgatcaca cccagatgaa tacccccatc
     1321 ggcggcggcc ccgtgcacgt gcccgagtac caccacatga gctaccacgt gaagctgagc
     1381 aaggatgtga ccgatcaccg cgataatatg agcctgacgg agaccgtgcg cgccgtggat
     1441 tgccgcaaga cctacctgta aattaaccaa actggatctc tgctgtccct gtaataaacc
     1501 cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct
     1561 attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa
     1621 ttgtggatga atactgccat ttgtctgcag aattggcgca cgcagtgccg atccgttcac
     1681 taatcgaatg gatctgtctc tgtctctctc tccaccttct tcttctattc cttcgggcct
     1741 gtcgggtccc ctcggggttg ggaggtgggt ctgaaacgat aatggtgaat atccctgcct
     1801 aactctattc actatagaaa gtacagcaaa aactattctt aaacctacca agcctcctac
     1861 tatcattatg aataatttta tataccacag ccaatttgtt atgttaaacc aattccacaa
     1921 acttgcccat ttatctaatt ccaataattc ttgttcattc ttttcttgct ggttttgcga
     1981 ttcttcaatt aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat
     2041 ccaggtcgtg tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg
     2101 cacagcagtg gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc
     2161 ctttaggtat ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt
     2221 gagttgcaac agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac
     2281 tataccagac aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt
     2341 gcttcctgct gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc
     2401 tctctgcacc actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac
     2461 tactttatat ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct
     2521 gaagatcagc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct
     2581 tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg
     2641 ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct
     2701 gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc
     2761 gttctagctc cctgcttgcc catactatat gttttaattt atattttttc tttccccctg
     2821 gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct
     2881 cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc
     2941 gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct
     3001 cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca
     3061 cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca
     3121 cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta
     3181 gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaacg
     3241 cgctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     3301 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     3361 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     3421 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     3481 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     3541 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     3601 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     3661 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     3721 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     3781 ccgtaattga ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc
     3841 gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg
     3901 tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca
     3961 cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag
     4021 tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca cttttcgggg
     4081 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4141 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4201 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4261 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4321 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4381 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4441 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4501 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4561 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4621 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4681 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4741 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4801 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4861 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4921 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4981 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5041 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5101 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5161 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5221 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5281 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5341 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5401 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5461 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5521 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5581 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5641 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5701 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5761 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5821 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     5881 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     5941 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     6001 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     6061 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     6121 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     6181 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga
     6241 ggtcgacggt atacagacat gataagatac attgatgagt ttggacaaac cacaactaga
     6301 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6361 attataagct gcaataaaca agttggggtg ggcgaagaac tccagcatga gatccccgcg
     6421 ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa
     6481 ggcggcggtg gaatcgaaat ctcgtagcac gtgtcagtcc tgctcctcgg ccacgaagtg
     6541 cacgcagttg ccggccgggt cgcgcagggc gaactcccgc ccccacggct gctcgccgat
     6601 ctcggtcatg gccggcccgg aggcgtcccg gaagttcgtg gacacgacct ccgaccactc
     6661 ggcgtacagc tcgtccaggc cgcgcaccca cacccaggcc agggtgttgt ccggcaccac
     6721 ctggtcctgg accgcgctga tgaacagggt cacgtcgtcc cggaccacac cggcgaagtc
     6781 gtcctccacg aagtcccggg agaacccgag ccggtcggtc cagaactcga ccgctccggc
     6841 gacgtcgcgc gcggtgagca ccggaacggc actggtcaac ttggccatgg tttagttcct
     6901 caccttgtcg tattatacta tgccgatata ctatgccgat gattaattgt caacacgtgc
     6961 tgatcagatc cgaaaatgga tatacaagct cccgggagct ttttgcaaaa gcctaggcct
     7021 ccaaaaaagc ctcctcacta cttctggaat agctcagagg cagaggcggc ctcggcctct
     7081 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     7141 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     7201 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     7261 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     7321 aactgacaca cattccacag aattaattcg cgttaaattt ttgttaaatc agctcatttt
     7381 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     7441 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     7501 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     7561 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     7621 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     7681 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     7741 ccgccgcgct taatgcgccg ctacagggcg cgtggggata ccccctagag ccccagctgg
     7801 ttctttccgc ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt
     7861 cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac
     7921 tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt
     7981 ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca
     8041 cagtcgaggc tgatcagcgg gtttaaacgg gccctgctag agattttcca cactgactaa
     8101 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     8161 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     8221 tcccaggctc agatctggtc taaccagaga gacccagtac agtccggatg cagctctcgg
     8281 gccatgtgat gaaatgctag gcggctgtca aacctccact ctaatacttc tctctccggg
     8341 tcatccatcc catgcaggct cacagggtgt aacaagcggg tgttctctcc ttcattggct
     8401 tcttctacct tctcttgctc aactggtact agcttgtagc accatccaaa ggtcagtgga
     8461 tatctgatcc ctggccctgg tgtgtagttc tgccaatcag ggaagtagcc ttgtgtgtgg
     8521 tagatccaca gatcaaggat atcttgtctt cgttgggagt gaattagccc ttccagtccc
     8581 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
     8641 ggtacctgag gtgtgactgg aaaacccacc tcctcctcct cttgtgcttc tagccaggca
     8701 caatcagcat tggtagctgc tgtattgcta cttgtgattg ctccatgttt ttctaggtct
     8761 cgaggtcgac ggtatcgatg cggggaggcg gcccaaaggg agatccgact cgtctgaggg
     8821 cgaaggcgaa gacgcggaag aggccgcaga gccggcag
//
