LOCUS       sequence-473923-        9056 bp    DNA     circular SYN 28-MAY-2025
DEFINITION  A synthetic GAL4 promoter comprising 14 tandem GAL4 binding sites 
            (G14p) regulating EYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9056)
  TITLE     Optimized Pipeline and Designer Cells for Synthetic Biology Based 
            High-Throughput Screening of Viral Protease Inhibitors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSE92: pFuGW-G14p-EYFP
FEATURES             Location/Qualifiers
     source          1..9056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     337..357
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             855..1559
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGDVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEIAGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLTE
                     TVRAVDCRKTYL"
     misc_feature    1601..1718
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2016..2060)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2245..2478
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2971..3096
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3143..3323
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3341..3539)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3366..3386)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3541..3920
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     4092..4111
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        4186..4290
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4291..4359,4360..5151)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4509..4528)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5322..5910
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5811..5830
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6064..6081
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6198..6219
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6234..6239,6240..6257,6258..6264)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6272..6288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6277..6299
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6296..6312
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6296..6312
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    6361..6482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6372..6391)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6426..6445
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(6612..6986)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(7005..7052)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(7100..7429)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7114..7249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7168..7187)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7414..7434
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(7443..7871)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7553..7574)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7765..7784
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7917..8141
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8130..8147
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             8173..8353
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     8858..8874
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     8859..8875
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(8878..9056,1..410)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             8984..8995
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ggaaaggacg atgatttccc cgacaacacc acggaattgt cagtgcccaa cagccgagcc
       61 cctgtccagc agcgggcaag gcaggcggcg atgagttccg ccgtggcaat agggaggggg
      121 aaagcgaaag tcccggaaag gagctgacag gtggtggcaa tgccccaacc agtgggggtt
      181 gcgtcagcaa acacagtgca caccacgcca cgttgcctga caacgggcca caactcctca
      241 taaagagaca gcaaccagga tttatacaag gaggagaaaa tgaaagccat acgggaagca
      301 atagcatgat acaaaggcat taaagcagcg tatccacata gcgtaaaagg agcaacatag
      361 ttaagaatac cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt atcgataagc
      421 ttgatatcga taggtaccga gtttctagac ggagtactgt cctccgagcg gagtactgtc
      481 ctccgactcg agcggagtac tgtcctccga tcggagtact gtcctccgcg aattccggag
      541 tactgtcctc cgaagacgct agacggagta ctgtcctccg agcggagtac tgtcctccga
      601 ctcgagcgga gtactgtcct ccggctagac ggagtactgt cctccgagcg gagtactgtc
      661 ctccgactcg agcggagtac tgtcctccgg ctagacggag tactgtcctc cgagcggagt
      721 actgtcctcc gactcgagcg gagtactgtc ctccggctag cggggggcta taaaaggggg
      781 tgggggcgtt cgtcctcact ctagatctgc gatctaagta agcttggcat tccggtactg
      841 ttggtaaagc caccatgagc agcggcgccc tgctgttcca cggcaagatc ccctacgtgg
      901 tggagatgga gggcgatgtg gatggccaca ccttcagcat ccgcggtaag ggctacggcg
      961 atgccagcgt gggcaaggtg gatgcccagt tcatctgcac caccggcgat gtgcccgtgc
     1021 cctggagcac cctggtgacc accctgacct acggcgccca gtgcttcgcc aagtacggcc
     1081 ccgagctgaa ggatttctac aagagctgca tgcccgatgg ctacgtgcag gagcgcacca
     1141 tcaccttcga gggcgatggc aatttcaaga cccgcgccga ggtgaccttc gagaatggca
     1201 gcgtgtacaa tcgcgtgaag ctgaatggcc agggcttcaa gaaggatggc cacgtgctgg
     1261 gcaagaatct ggagttcaat ttcacccccc actgcctgta catctggggc gatcaggcca
     1321 atcacggcct gaagagcgcc ttcaagatct gccacgagat cgccggcagc aagggcgatt
     1381 tcatcgtggc cgatcacacc cagatgaata cccccatcgg cggcggcccc gtgcacgtgc
     1441 ccgagtacca ccacatgagc taccacgtga agctgagcaa ggatgtgacc gatcaccgcg
     1501 ataatatgag cctgacggag accgtgcgcg ccgtggattg ccgcaagacc tacctgtaaa
     1561 ttaaccaaac tggatctctg ctgtccctgt aataaacccg aaaattttga atttttgtaa
     1621 tttgtttttg taattcttta gtttgtatgt ctgttgctat tatgtctact attctttccc
     1681 ctgcactgta ccccccaatc cccccttttc ttttaaaatt gtggatgaat actgccattt
     1741 gtctgcagaa ttggcgcacg cagtgccgat ccgttcacta atcgaatgga tctgtctctg
     1801 tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg
     1861 aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt
     1921 acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata
     1981 taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc
     2041 aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt
     2101 aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc
     2161 tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt
     2221 tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc
     2281 aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc
     2341 gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg
     2401 gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac
     2461 ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt
     2521 gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt atataattca
     2581 cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg ccgcttgctg
     2641 tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt ccttggtgtc
     2701 ttttatctct atcctttgat gcacacaata gagggttgct actgtattat ataatgatct
     2761 aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt gtctacagcc
     2821 ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc tgcttgccca
     2881 tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa ttttttccca
     2941 tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc tctccttcta
     3001 gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc ctcttgccgt
     3061 gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc ctttcgcttt
     3121 caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa gggtctgagg
     3181 gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc
     3241 aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag
     3301 atctggtcta accagagaga cccagtacag gcaaaacgcg ctgcttatat agacctccca
     3361 ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa
     3421 gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg
     3481 aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac
     3541 catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt
     3601 catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt
     3661 acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac
     3721 ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga
     3781 cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt
     3841 aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt actattaata
     3901 actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa
     3961 acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc
     4021 ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg
     4081 gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga
     4141 tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4201 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4261 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4321 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4381 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4441 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4501 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4561 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4621 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4681 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4741 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4801 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4861 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4921 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4981 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     5041 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5101 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5161 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5221 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5281 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5341 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5401 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5461 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5521 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5581 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5641 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5701 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5761 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5821 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5881 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5941 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     6001 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6061 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6121 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6181 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6241 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6301 aacagctatg accatgatta cgccaagctc tagctagagg tcgacggtat acagacatga
     6361 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     6421 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     6481 ttggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg
     6541 tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct
     6601 cgtagcacgt gtcagtcctg ctcctcggcc acgaagtgca cgcagttgcc ggccgggtcg
     6661 cgcagggcga actcccgccc ccacggctgc tcgccgatct cggtcatggc cggcccggag
     6721 gcgtcccgga agttcgtgga cacgacctcc gaccactcgg cgtacagctc gtccaggccg
     6781 cgcacccaca cccaggccag ggtgttgtcc ggcaccacct ggtcctggac cgcgctgatg
     6841 aacagggtca cgtcgtcccg gaccacaccg gcgaagtcgt cctccacgaa gtcccgggag
     6901 aacccgagcc ggtcggtcca gaactcgacc gctccggcga cgtcgcgcgc ggtgagcacc
     6961 ggaacggcac tggtcaactt ggccatggtt tagttcctca ccttgtcgta ttatactatg
     7021 ccgatatact atgccgatga ttaattgtca acacgtgctg atcagatccg aaaatggata
     7081 tacaagctcc cgggagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     7141 tctggaatag ctcagaggca gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     7201 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     7261 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     7321 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     7381 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagaa
     7441 ttaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     7501 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     7561 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7621 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     7681 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7741 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7801 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7861 acagggcgcg tggggatacc ccctagagcc ccagctggtt ctttccgcct cagaagccat
     7921 agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc ccttgctgtc
     7981 ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg atgcaatttc
     8041 ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg aaggcacggg
     8101 ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg atcagcgggt
     8161 ttaaacgggc cctgctagag attttccaca ctgactaaaa gggtctgagg gatctctagt
     8221 taccagagtc acacaacaga cgggcacaca ctacttgaag cactcaaggc aagctttatt
     8281 gaggcttaag cagtgggttc cctagttagc cagagagctc ccaggctcag atctggtcta
     8341 accagagaga cccagtacag tccggatgca gctctcgggc catgtgatga aatgctaggc
     8401 ggctgtcaaa cctccactct aatacttctc tctccgggtc atccatccca tgcaggctca
     8461 cagggtgtaa caagcgggtg ttctctcctt cattggcttc ttctaccttc tcttgctcaa
     8521 ctggtactag cttgtagcac catccaaagg tcagtggata tctgatccct ggccctggtg
     8581 tgtagttctg ccaatcaggg aagtagcctt gtgtgtggta gatccacaga tcaaggatat
     8641 cttgtcttcg ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc
     8701 taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt gtgactggaa
     8761 aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg gtagctgctg
     8821 tattgctact tgtgattgct ccatgttttt ctaggtctcg aggtcgacgg tatcgatgcg
     8881 gggaggcggc ccaaagggag atccgactcg tctgagggcg aaggcgaaga cgcggaagag
     8941 gccgcagagc cggcagcagg ccgcgggaag gaaggtccgc tggattgagg gccgaaggga
     9001 cgtagcagaa ggacgtcccg cgcagaatcc aggtggcaac acaggcgagc agccaa
//
