LOCUS       sequence-474066-         480 bp    DNA     linear   UNA 30-MAY-2025
DEFINITION  bacterial expression of human TTR mutants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Sun X, Ferguson JA, Leach BI, Stanfield RL, Dyson HJ, Wright PE
  TITLE     Probing the Dissociation Pathway of a Kinetically Labile 
            Transthyretin Mutant.
  JOURNAL   J Am Chem Soc. 2024 Jan 10;146(1):532-542. doi: 
            10.1021/jacs.3c10083. Epub 2023 Dec 22.
  PUBMED    38134439
REFERENCE   2  (bases 1 to 480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c10083"; journalName: "J Am Chem Soc"; date: 
            "2024-01-10- 10"; volume: "146"; issue: "1"; pages: "532-542"
COMMENT     Sequence Label: TTR A25T
FEATURES             Location/Qualifiers
     source          1..480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    4..28
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             43..65
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
       61 ggagatatac atatgggtcc tacgggcacc ggtgaatcca agtgtcctct gatggtcaaa
      121 gttctagatg ctgtccgagg cagtcctacc atcaatgtgg ccgtgcatgt gttcagaaag
      181 gctgctgatg acacctggga gccatttgcc tctgggaaaa ccagtgagtc tggagagctg
      241 catgggctca caactgagga ggaatttgta gaagggatat acaaagtgga aatagacacc
      301 aaatcttact ggaaggcact tggcatctcc ccattccatg agcatgcaga ggtggtattc
      361 acagccaacg actccggccc ccgccgctac accattgccg ccctgctgag cccctactcc
      421 tattccacca cggctgtcgt caccaatccc aaggaatgag atccggctgc tacaaagccc
//
