LOCUS       sequence-474067-         560 bp    DNA     linear   UNA 30-MAY-2025
DEFINITION  bacterial expression of human TTR mutants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Sun X, Ferguson JA, Leach BI, Stanfield RL, Dyson HJ, Wright PE
  TITLE     Probing the Dissociation Pathway of a Kinetically Labile 
            Transthyretin Mutant.
  JOURNAL   J Am Chem Soc. 2024 Jan 10;146(1):532-542. doi: 
            10.1021/jacs.3c10083. Epub 2023 Dec 22.
  PUBMED    38134439
REFERENCE   2  (bases 1 to 560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.3c10083"; journalName: "J Am Chem Soc"; date: 
            "2024-01-10- 10"; volume: "146"; issue: "1"; pages: "532-542"
COMMENT     Sequence Label: TTR C10S/S85C/A25T
FEATURES             Location/Qualifiers
     source          1..560
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        65..83
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..145
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatcgatct cgatcccgcg
       61 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
      121 attttgttta actttaagaa ggagatatac atatgggtcc tacgggcacc ggtgaatcca
      181 agagtcctct gatggtcaaa gttctagatg ctgtccgagg cagtcctacc atcaatgtgg
      241 ccgtgcatgt gttcagaaag gctgctgatg acacctggga gccatttgcc tctgggaaaa
      301 ccagtgagtc tggagagctg catgggctca caactgagga ggaatttgta gaagggatat
      361 acaaagtgga aatagacacc aaatcttact ggaaggcact tggcatctgc ccattccatg
      421 agcatgcaga ggtggtattc acagccaacg actccggccc ccgccgctac accattgccg
      481 ccctgctgag cccctactcc tattccacca cggctgtcgt caccaatccc aaggaatgag
      541 atccggctgc tacaaagccc
//
