LOCUS       sequence-474109-        4633 bp    DNA     circular SYN 30-MAY-2025
DEFINITION  Transfection backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4633)
  TITLE     Promega Early Access Collection
REFERENCE   2  (bases 1 to 4633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFC37A HiBiT CMV-neo Flexi Vector
FEATURES             Location/Qualifiers
     source          1..4633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..21)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          4..136
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(272..475)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(301..321)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        476..855
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    1046..1329
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     rep_origin      1445..2033
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1934..1953
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2191..2982,2983..3051))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2814..2833
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3052..3156)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    3312..3360
                     /note="synthetic polyadenylation signal"
     CDS             complement(3424..4218)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3535..3554)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4145..4164
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4269..4626)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4283..4418
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        4435..4626
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4583..4603
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cacctgtgga gagaaaggca aagtggatgt cagtaagacc aataggtgcc tatcagaaac
       61 gcaagagtct tctctgtctc gacaagccca gtttctattg gtctccttaa acctgtcttg
      121 taaccttgat acttacctgc ccagtgcctc acgaccaact tctgcagctt aagttcgaga
      181 ctgttgtgtc agaagcactg actgcgttag caatttaact gtgataaact accgcaataa
      241 agcttctagt gatctgacgg ttcactaaac cagctctgct tatatagacc tcccaccgta
      301 cacgcctacc gcccatttgc gtcaacgggg cggggttatt acgacatttt ggaaagtccc
      361 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
      421 cccgtgagtc aaaccgctat ccacgcccat tggtgtactg ccaaaaccgc atcaccatgg
      481 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccgt aaggtcatgt
      541 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcggacttg
      601 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
      661 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
      721 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
      781 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
      841 tcaataatca atgccaacat ggcggtcata ttggacatga gccaatataa atgtacatat
      901 tatgatatag atacaacgta tgcaatggcc aatagccaat attgatttat gctatataac
      961 caatgaataa tatggctaat ggccaatatt gaagatctcg atcctctacg ccggacgcat
     1021 cgtggccgga gtactgcggt ttatctcgat ggctacgagg gcagacagta agtggattta
     1081 ccataatccc ttaattgtac gcaccgctaa aacgcgttca gcgcgatcac ggcagcagac
     1141 aggtaaaaat ggcaacaaac cacccgaaaa actgccgcaa tcgcgcctga taaattttaa
     1201 ccgtatgaat acctatgcaa ccagagggta caggccacat tacccccact taatccactg
     1261 aagctgccat ttttcatggt ttcaccatcc cagcgaaggg ccatccagcg tgcgttcctg
     1321 tatttccgga ctataaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1441 cttcttgaaa tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1561 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2161 gttgggcgtc gcttggtcgg tcatttcgaa ttaccaatgc ttaatcagtg aggcacctat
     2221 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2281 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2341 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2401 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2461 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2521 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2581 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatagttgt
     2641 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2701 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2761 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     2821 cgcgccacat agcagaactt tgaaagtgct catcattgga aaacgttctt cggggcgaaa
     2881 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     2941 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3001 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3061 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3121 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3181 tgacgtctaa gaaaccatta cgtagaaagc cagtccgcag aaacggtgct gaccccggat
     3241 gaatgtcagc tactgggcta tctggacaag ggaaaacgca agcgcaaaga ggatccttat
     3301 cgctatcgat tcacacaaaa aaccaacaca cagatgtaat gaaaataaag atattttatt
     3361 gcggccatcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc
     3421 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     3481 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     3541 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     3601 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     3661 cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg
     3721 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     3781 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     3841 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     3901 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     3961 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     4021 ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     4081 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     4141 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     4201 tccatcttgt tcaatcatgg tggctctagc cttaagttcg agactgttgt gtcagaagaa
     4261 tcaagctttt tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc
     4321 tcagaggccg aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc
     4381 ggagaatggg cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact
     4441 atggttgctg actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg
     4501 gactttccac acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4561 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagctg gttctttccg
     4621 cctcaggagt gga
//
