LOCUS       sequence-474314-       14133 bp    DNA     circular SYN 03-JUN-2025
DEFINITION  Expresses human SETD5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14133)
  AUTHORS   Sashiyama S, Nakagawa T, Nakagawa M, Hosogane M, Watanabe Y, 
            Ashitomi H, Yamane K, Shibuya N, Moroishi T, Nakayama K, Hosoi T
  TITLE     KBG syndrome-associated protein ANKRD11 regulates SETD5 expression 
            to modulate rRNA levels and translation
  JOURNAL   iScience 2025 May 19, 112699
REFERENCE   2  (bases 1 to 14133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience 
            2025 May 19, 112699"
COMMENT     Sequence Label: PB-TA-ERN-SETD5
FEATURES             Location/Qualifiers
     source          1..14133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     350..369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1140..1158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1177..1195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1214..1232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1251..1269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1288..1306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1325..1343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1362..1380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1399..1417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1439..1463
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    1542..1566
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5956..5980)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5982..6005
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(6036..6054)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     protein_bind    complement(6396..6420)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6422..6445
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(6476..6494)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    6520..6744
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        6841..7220
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7335..8640
                     /label=rEF-1-alpha promoter
                     /note="strong constitutive promoter for rat elongation 
                     factor EF-1-alpha"
     misc_feature    7677..8608
                     /label=rEF-1-alpha intron
                     /note="intron upstream of the start codon of rat 
                     EF-1-alpha"
     CDS             join(8657..9274,9275..9280,9281..9316,9317..9319,9320..9355,
                     9356..9358,9359..9394,9395..9403)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    9430..9874
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        9907..9953
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        9945..9963
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9973..10767
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9973..10767
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10027..10046)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10637..10656
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10814..11386
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     repeat_region   11760..11794
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(11943..11961)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11982..11998)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11982..11998)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11995..12017)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12006..12022
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12030..12036,12037..12054,12055..12060))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12075..12096
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12213..12230)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12384..12972)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12464..12483)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13143..13934,13935..14003))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13766..13785
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14004..14108)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      961 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     1021 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     1081 cacatatcaa tattatgctc tcgttaatta actcgaggga tccaagctgg ccgcacgtct
     1141 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta
     1201 cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag
     1261 tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc gacacgtctc
     1321 gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga
     1381 agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat ataagcagag
     1441 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1501 gaagacaccg ggaccgatcc agcctccgcg gtggcggcat cacaagtttg tacaaaaaag
     1561 caggctcttt aaaggaacca attcagtcga ctggatccgg taccatgagc attgcaatcc
     1621 ctctgggagt caccacatca gatacatcct actcagatat ggctgctgga tcagaccctg
     1681 aatctgtgga ggctagtcca gcagttaatg agaagagcgt gtattccact cataattatg
     1741 ggaccactca gaggcatggg tgtcgaggac tgccttatgc tacgatcatc cctcgttctg
     1801 acctgaatgg cctgccgtcg cctgtagagg aacgctgtgg agacagcccg aactctgaag
     1861 gagaaactgt acctacctgg tgtccttgtg gtctttctca ggatggcttc cttctcaact
     1921 gtgacaagtg caggggaatg agcaggggga aggttattag acttcatcgg cggaagcagg
     1981 acaacatatc aggtggggat agcagtgcaa cagaaagctg ggatgaggag ctttctcctt
     2041 ccactgtgtt gtatacagca acacagcaca cacctacaag catcacctta actgttagaa
     2101 gaaccaaacc caagaagcgg aaaaagagtc cagaaaaggg tcgtgcagca ccaaagacga
     2161 agaaaatcaa gaattctccc tctgaagcac agaatttaga tgagaataca actgagggct
     2221 gggaaaatcg gataagacta tggactgacc agtatgaaga agctttcact aatcagtaca
     2281 gtgcagatgt acagaacgcg cttgaacaac acctacattc tagcaaggaa tttgtgggca
     2341 aacctactat tttagacact attaataaga ctgaattggc ctgtaataac acagttattg
     2401 gttcccaaat gcagttacag ctgggaagag tcactcgtgt tcaaaagcac cggaagatcc
     2461 tgagggctgc aagagatttg gctttggaca ctcttataat agagtatcgt gggaaagtca
     2521 tgttacgaca gcaatttgag gtcaatgggc atttcttcaa aaaaccatac ccctttgtgc
     2581 tcttctactc aaaattcaat ggtgtagaga tgtgtgtgga tgcccgtact ttcggtaatg
     2641 atgctcggtt catcagaaga tcatgtacac caaatgcaga ggtgcgacac atgattgcag
     2701 atgggatgat tcacctgtgc atctatgctg tgtctgccat caccaaggat gctgaggtca
     2761 ccatagcatt tgattatgag tatagtaact gtaattataa agtggactgt gcctgtcaca
     2821 agggaaaccg gaattgtcct atacaaaaaa ggaatcctaa tgctacagaa ctgccactcc
     2881 taccacctcc tccaagccta cccaccattg gagcagagac tagacgtaga aaagcacgac
     2941 ggaaagagct agagatggag cagcagaatg aggcttcaga ggagaataat gaccagcaat
     3001 cacaagaagt tccagaaaaa gtaactgtat ccagtgatca tgaggaagta gacaatccag
     3061 aagaaaaacc agaagaagag aaagaagagg ttatagatga ccaggagaac ctagctcata
     3121 gcaggaggac cagggaagat agaaaggtag aagccatcat gcatgctttt gaaaacttag
     3181 agaaaagaaa gaagcggcgg gatcagccct tggaacagag caactctgat gtagagatta
     3241 ctacaaccac ctcagagact cctgttggtg aagagacaaa aactgaagcc cctgaatctg
     3301 aagttagcaa ctctgtttca aatgttacca tcccaagcac cccacagagt gttggtgtga
     3361 atacccggag gtcttcccaa gcaggggata ttgctgcaga aaaactagtc cccaagccac
     3421 ctccagcaaa gccttctagg ccccggccga agagtcgaat ttctcggtac aggaccagtt
     3481 cagcccaaag actaaagcgt cagaagcagg ccaatgcaca gcaggcagaa ttgtcacaag
     3541 ctgccttgga agagggagga agtaacagtt tagtaactcc tactgaagct ggaagtctag
     3601 acagttcagg agaaaacagg ccattaacag ggtctgaccc aactgtggtg tcaattactg
     3661 gatcccatgt caaccgtgct gcatctaaat accccaaaac caaaaagtat ctagttacag
     3721 aatggttgaa tgacaaagca gagaagcaag agtgccctgt tgagtgccct ttacgtatca
     3781 caacggatcc aactgtactg gcaacgaccc taaacatgtt accaggtctt atccattccc
     3841 cgttaatttg caccaccccc aaacactaca ttcgctttgg ctcacccttt atccctgaga
     3901 gacgtcgaag gccccttctg cctgatggca cattcagctc ctgtaagaag cgctggataa
     3961 aacaagcctt agaagaaggg atgactcaaa catcatctgt accccaagag actagaactc
     4021 agcacctata ccaaagcaat gagaatagta gctcttctag tatctgcaaa gacaatgcag
     4081 acttgttgag cccattaaag aaatggaagt ctcgctatct gatggagcag aatgtcacca
     4141 agttacttcg gcctctgtct ccagtcacac caccccctcc caattcaggc tcaaagagtc
     4201 cccagctggc cacacctggc tcatctcacc caggagaaga ggagtgtcga aatggataca
     4261 gcctcatgtt ttcaccagtc acatctctta ctactgctag tcgctgcaac actcctctac
     4321 agtttgagct ttgtcaccga aaagacctgg atttggcaaa agtaggatac cttgactcca
     4381 acactaacag ctgtgctgat agaccttccc tactcaactc aggtcattct gacctggctc
     4441 ctcatccctc cctcggaccc acttctgaga ctggtttccc aagcagaagt ggagatggac
     4501 atcagaccct cgtgagaaac tcagaccagg catttcggac agagttcaac ttgatgtatg
     4561 cctactcccc tttgaatgct atgcctcgag cagatggact gtatcgagga tctcctctag
     4621 tgggggatag gaagccttta catttggatg ggggatattg ttcccctgca gaaggatttt
     4681 ccagcagata tgaacatggc ttaatgaaag acctctctcg tggatccttg tcacctggtg
     4741 gtgaaagggc ctgtgaagga gtcccatctg ccccccagaa cccaccacag aggaaaaaag
     4801 tatccctgct ggagtaccga aaacggaaac aagaagctaa ggaaaattct gctggtgggg
     4861 gaggtgactc tgcacagagc aaaagcaagt ctgcaggagc tgggcaaggc agcagtaact
     4921 ccgtttccga caccggtgcc catggtgtgc agggatcctc agcccgaact ccatcttccc
     4981 ctcacaaaaa attctcccca tctcattcct ctatgtccca tttggaggcg gtaagcccat
     5041 cagattccag aggcacttct tcatctcact gcagacctca agagaatatc agcagtaggt
     5101 ggatggttcc cacatcagta gaacgactcc gagaaggagg gagcatcccc aaggtcctcc
     5161 gaagcagcgt gagggtggcc caaaagggag agccctctcc cacatgggag agtaacatca
     5221 cagagaaaga ctcagaccct gcagatggag aaggcccaga gacattaagc tcagcactct
     5281 ctaaaggagc aacagtttac agcccttcca gatacagcta ccagctcctg cagtgtgata
     5341 gtcctcggac agaatcacaa agcctccttc agcagagttc ctcccccttc agaggacatc
     5401 ctacacagtc tccaggatac agttatcgaa ctactgcact gagacctgga aaccccccct
     5461 ctcacggttc ttcagaatca tccctctctt ccacgtccta ttccagcccc gcccaccctg
     5521 tgtccacaga ctcgttggcc ccatttacgg ggacaccagg gtattttagc agccagccac
     5581 attctggaaa cagcactggc agcaatcttc caaggaggag ctgcccttct agtgctgcta
     5641 gccctaccct gcagggaccc tcagactcgc caacctcaga ttcagtttct cagtccagca
     5701 caggaactct gagttccacc tcctttcctc agaactctag gtcgtcattg ccatcagact
     5761 tacggactat cagtctgccc agtgctgggc agtcagctgt ctaccaggcc tccagggtat
     5821 ctgcggtttc caattcacag cactacccac accgtgggag tgggggtgtg caccagtacc
     5881 gactccagcc actgcaaggg tcaggagtca agactcagac gggactttcc tcgcggccgc
     5941 actcgagata tctagaccca gctttcttgt acaaagtggt tgactacaaa gacgatgacg
     6001 acaagtgact cgagcatgca tctagagggc cctattctat agtgtcacct aaatgctaga
     6061 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     6121 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttcctn ngaactctag
     6181 gtcgtcattg ccatcagact tacggactat cagtctgccc agtgctgggc agtcagctgt
     6241 ctaccaggcc tccagggtat ctgcggtttc caattcacag cactacccac accgtgggag
     6301 tgggggtgtg caccagtacc gactccagcc actgcaaggg tcaggagtca agactcagac
     6361 gggactttcc tcgcggccgc actcgagata tctagaccca gctttcttgt acaaagtggt
     6421 tgactacaaa gacgatgacg acaagtgact cgagcatgca tctagagggc cctattctat
     6481 agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     6541 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     6601 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     6661 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     6721 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagatct gtacattcga
     6781 atcgatgaat tcgttaacca tggaagcttc tgcagcggcc gcactagcgg tggcggcctc
     6841 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     6901 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     6961 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7021 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7081 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7141 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7201 tagtcatcgc tattaccatg atgatgcggt tttggcagta catcaatggg cgtggatagc
     7261 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7321 gactagtgga gccgagagta attcatacaa aaggagggat cgccttcgca aggggagagc
     7381 ccagggaccg tccctaaatt ctcacagacc caaatccctg tagccgcccc acgacagcgc
     7441 gaggagcatg cgcccagggc tgagcgcggg tagatcagag cacacaagct cacagtcccc
     7501 ggcggtgggg ggaggggcgc gctgagcggg ggccagggag ctggcgcggg gcaaactggg
     7561 aaagtggtgt cgtgtgctgg ctccgccctc ttcccgaggg tgggggagaa cggtatataa
     7621 gtgcggtagt cgccttggac gttctttttc gcaacgggtt tgccgtcaga acgcaggtga
     7681 gtggcgggtg tggcttccgc gggccccgga gctggagccc tgctctgagc gggccgggct
     7741 gatatgcgag tgtcgtccgc agggtttagc tgtgagcatt cccacttcga gtggcgggcg
     7801 gtgcgggggt gagagtgcga ggcctagcgg caaccccgta gcctcgcctc gtgtccggct
     7861 tgaggcctag cgtggtgtcc gccgccgcgt gccactccgg ccgcactatg cgttttttgt
     7921 ccttgctgcc ctcgattgcc ttccagcagc atgggctaac aaagggaggg tgtggggctc
     7981 actcttaagg agcccatgaa gcttacgttg gataggaatg gaagggcagg aggggcgact
     8041 ggggcccgcc cgccttcgga gcacatgtcc gacgccacct ggatggggcg aggcctgtgg
     8101 ctttccgaag caatcgggcg tgagtttagc ctacctgggc catgtggccc tagcactggg
     8161 cacggtctgg cctggcggtg ccgcgttccc ttgcctccca acaagggtga ggccgtcccg
     8221 cccggcacca gttgcttgcg cggaaagatg gccgctcccg gggccctgtt gcaaggagct
     8281 caaaatggag gacgcggcag cccggtggag cgggcgggtg agtcacccac acaaaggaag
     8341 agggccttgc ccctcgccgg ccgctgcttc ctgtgacccc gtggtctatc ggccgcatag
     8401 tcacctcggg cttctcttga gcaccgctcg tcgcggcggg gggaggggat ctaatggcgt
     8461 tggagtttgt tcacatttgg tgggtggaga ctagtcaggc cagcctggcg ctggaagtca
     8521 ttcttggaat ttgccccttt gagtttggag cgaggctaat tctcaagcct cttagcggtt
     8581 caaaggtatt ttctaaaccc gtttccaggt gttgtgaaag ccaccgctaa ttcaaagcaa
     8641 tccggagtaa ttcaccatgt ctagactgga caagagcaaa gtcataaacg gcgctctgga
     8701 attactcaat ggagtcggta tcgaaggcct gacgacaagg aaactcgctc aaaagctggg
     8761 agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc tcgatgccct
     8821 gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag gcgagtcatg
     8881 gcaagacttt ctgcggaaca acgccaagtc attccgctgt gctctcctct cacatcgcga
     8941 cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa ccctggaaaa
     9001 tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt acgctctgtc
     9061 cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc aagtagcaaa
     9121 agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac aagcaattga
     9181 gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac taatcatatg
     9241 tggcctggag aaacagctaa agtgcgaaag cggcgggccg gccgacgccc ttgacgattt
     9301 tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata tgctgcctgc
     9361 tgacgctctt gacgattttg accttgacat gctccccggg taactaagta aggatcccgt
     9421 acgcctagga gcaggtttcc ccaatgacac aaaacgtgca acttgaaact ccgcctggtc
     9481 tttccaggtc tagaggggta acactttgta ctgcgtttgg ctccacgctc gatccactgg
     9541 cgagtgttag taacagcact gttgcttcgt agcggagcat gacggccgtg ggaactcctc
     9601 cttggtaaca aggacccacg gggccaaaag ccacgcccac acgggcccgt catgtgtgca
     9661 accccagcac ggcgacttta ctgcgaaacc cactttaaag tgacattgaa actggtaccc
     9721 acacactggt gacaggctaa ggatgccctt caggtacccc gaggtaacac gcgacactcg
     9781 ggatctgaga aggggactgg ggcttctata aaagcgctcg gtttaaaaag cttctatgcc
     9841 tgaataggtg accggaggtc ggcacctttc ctttgcaatt actgacccta tgaatacact
     9901 gactgtttga caattaatca tcggcatagt atatcggcat agtataatac gactcactat
     9961 aggagggcca ccatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
    10021 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
    10081 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
    10141 ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
    10201 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
    10261 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
    10321 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
    10381 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
    10441 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
    10501 agcatgcccg acggcgagga tctcgtcgtg acacatggcg atgcctgctt gccgaatatc
    10561 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
    10621 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
    10681 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
    10741 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
    10801 cgaattcgct aggattatcc ctaatacctg ccaccccact cttaatcagt ggtggaagaa
    10861 cggtctcaga actgtttgtt tcaattggcc atttaagttt agtagtaaaa gactggttaa
    10921 tgataacaat gcatcgtaaa accttcagaa ggaaaggaga atgttttgtg gaccactttg
    10981 gttttctttt ttgcgtgtgg cagttttaag ttattagttt ttaaaatcag tactttttaa
    11041 tggaaacaac ttgaccaaaa atttgtcaca gaattttgag acccattaaa aaagttaaat
    11101 gagaaacctg tgtgttcctt tggtcaacac cgagacattt aggtgaaaga catctaattc
    11161 tggttttacg aatctggaaa cttcttgaaa atgtaattct tgagttaaca cttctgggtg
    11221 gagaataggg ttgttttccc cccacataat tggaagggga aggaatatca tttaaagcta
    11281 tgggagggtt gctttgatta caacactgga gagaaatgca gcatgttgct gattgcctgt
    11341 cactaaaaca ggccaaaaac tgagtccttg ggttgcatag aaagctgcca aacgctagcc
    11401 aacaagctcg tcatcgcttt gcagaagagc agagaggata tgctcatcgt ctaaagaact
    11461 acccatttta ttatatatta gtcacgatat ctataacaag aaaatatata tataataagt
    11521 tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa atcttaaaag
    11581 tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt tcaaaatcag
    11641 tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga ctgagatgtc
    11701 ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt tcaagaatgc
    11761 atgcgtcaat tttacgcaga ctatctttct agggttaaaa aagatttgcg ctttactcga
    11821 cctaaacttt aaacacgtca tagaatcttc gtttgacaaa aaccacattg tggccaagct
    11881 gtgtgacgcg acgcgcgcta aagaatggca aaccaagtcg cgcgaggtac cagcttttgt
    11941 tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg
    12001 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
    12061 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
    12121 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
    12181 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
    12241 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
    12301 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
    12361 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
    12421 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
    12481 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
    12541 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
    12601 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
    12661 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
    12721 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
    12781 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
    12841 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
    12901 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
    12961 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
    13021 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
    13081 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
    13141 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
    13201 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
    13261 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
    13321 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
    13381 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
    13441 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
    13501 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
    13561 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    13621 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    13681 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    13741 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    13801 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    13861 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    13921 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    13981 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
    14041 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
    14101 gttccgcgca catttccccg aaaagtgcca cct
//
