LOCUS       sequence-474321-        1927 bp    DNA     circular SYN 03-JUN-2025
DEFINITION  PiggyBac plasmid for the insertion of a sperm-expressed red 
            fluorescent protein in Aedes aegypti.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1927)
  TITLE     Mosquito sex under lock and key
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 3, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PB{3xP3-EGFP; beta-2Tubulin-dsRed2}
FEATURES             Location/Qualifiers
     source          1..1927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    185..306
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(196..215)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     250..269
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(427..1104)
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMSFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(489..512)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     904..924
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     regulatory      1101..1110
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tgatatttct aggttttcca ggcttggaaa aaaatccagg tttaagaaag tttacaacat
       61 acacaaatta ttgatgaatt tttcatttta gataagtagt aatcattttc aaagctagga
      121 gttttgcttc gaaatactta aacaaaaaat ctaaggggcg cgccgtacgc gtatcgataa
      181 gctttaagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
      241 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
      301 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
      361 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tctagagtcg
      421 cggccgctac aggaacaggt ggtggcggcc ctcggtgcgc tcgtactgct ccacgatggt
      481 gtagtcctcg ttgtgggagg tgatgtccag cttggcgtcc acgtagtagt agccgggcag
      541 ctgcacgggc ttcttggcca tgtagatgga cttgaactcc accaggtagt ggccgccgtc
      601 cttcagcttc agggccttgt gggtctcgcc cttcagcacg ccgtcgcggg ggtacaggcg
      661 ctcggtggag gcctcccagc ccatggtctt cttctgcatc acggggccgt cggaggggaa
      721 gttcacgccg atgaacttca ccttgtagat gaagcagccg tcctgcaggg aggagtcctg
      781 ggtcacggtc gccacgccgc cgtcctcgaa gctcatcacg cgctcccact tgaagccctc
      841 ggggaaggac agcttcttgt agtcggggat gtcggcgggg tgcttcacgt acaccttgga
      901 gccgtactgg aactgggggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt
      961 caccttcagc ttcacggtgt tgtggccctc gtaggggcgg ccctcgccct cgccctcgat
     1021 ctcgaactcg tggccgttca cggtgccctc catgcgcacc ttgaagcgca tgaactcggt
     1081 gatgacgttc tcggaggagg ccatggtggc gaccggtgga tcccctggag cacttctagc
     1141 ggttccaaaa ttgggcttca aaatatgctt cttccgatgc ttgaagatgg aagcagaatg
     1201 gttccaaatc acgtccgcgt tcctcggtgg tgaaaatcga agtgaaaatt tttccaactg
     1261 tcgtcctccg ggtcgtcaag aagaaggatg gccttccatg acacactgtg attgatttac
     1321 attgtggcgg cagtatgggc ccccaaaacg ggagcgacgc gttcaacatg tgctactgga
     1381 tgcattcgct tcgaagtctg ttgtcacgtt gggggctgtc catatgccac gtggttgtta
     1441 tggggtggag aagggtactg ctcaagcatc acggtgcata caaaatttga aaatttttgt
     1501 gtggacgaaa gatcataagg aggagcgtga aaattcataa aaaatgacca cgtggtttat
     1561 ggacgaaaca aaagcgatat atgagccaca agaacatagc gtgattcacc cccacattag
     1621 gggaaacatg caagtccttt gcataaacta tcaaaatttg gcggaaaata atagtgttga
     1681 aatgaataat aacagttaca actaggtatt agttcttcct cttttcggca ttacgtctcc
     1741 tttgtggcag agcatgtttc tgagtttaaa aaccatcatt tattttttgt tgattgctga
     1801 tgattgaaga ttgaatgata gatttttgaa ccttaaaaca aaaaagttaa cgcgatttaa
     1861 actattatga ggggtttaaa aattctaacg ttcagagtgt ctactaattt acagaaatga
     1921 aattccc
//
