LOCUS       sequence-474368-       10130 bp    DNA     circular SYN 05-AUG-2025
DEFINITION  Expresses CDK11A with G567S resistance mutation in mammalian cell 
            lines.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10130)
  AUTHORS   Julian LA, Crozier L, Lukow D, Mishra S, Swamy A, Hagenson RA, 
            Sennhenn P, Sausville EL, Mendelson B, Chuaqui C, Qiao L, Vasudevan 
            A, Lin K-T, Bhatia S, Bertomeu T, Chatr-aryamontri A, Zhang L, Rees 
            MG, Ronan MM, Roth JA, Nottoli T, Bai S, Lakshmipathi J, Muthusamy 
            V, Van Vranken JG, Gygi SP, Thompson SL, Smith JC, Anderson K, Shah 
            S, Bindra RS, Akerman M, Spector DL, Krainer AR, Sheltzer JM
  TITLE     On-target toxicity limits the efficacy of CDK11 inhibition against 
            cancers with 1p36 deletions
  JOURNAL   bioRxiv 2025.08.03.668359; doi: 
            https://doi.org/10.1101/2025.08.03.668359
REFERENCE   2  (bases 1 to 10130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.08.03.668359; doi: https://doi.org/10.1101/2025.08.03.668359"
COMMENT     Sequence Label: pLV[Exp]-Bsd-CMV>hCDK11A G567S
FEATURES             Location/Qualifiers
     source          1..10130
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2858..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2993..3013
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        3074..3377
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3378..3581
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3532..3552
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3602..3626
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3627..3636
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5985..6009)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6027..6615
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6080..6100)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6498..6509)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6646..7145
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6681..6704)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7078..7097
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        7155..7202
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7221..7619
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             7706..7939
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8011..8132
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8048..8067)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8102..8121
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8172..8307
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8327..8346)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8328..8346)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8356..8373)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8356..8372)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8365..8387)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8514..8969
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8601..8620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8811..8832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8995..9099
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9100..9168,9169..9960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9318..9337)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaaga
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cgctagcttt taaaagaaaa ggggggattg
     2881 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     2941 aattacaaaa acaaattaca aaaattcaaa attttactag tgattatcgg atcaactttg
     3001 tatagaaaag ttgtagttat taatagtaat caattacggg gtcattagtt catagcccat
     3061 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3121 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3181 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3241 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3301 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3361 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3421 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3481 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     3541 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     3601 tccaagtttg tacaaaaaag caggctgcca ccatgggtga tgaaaaggac tcttggaaag
     3661 tgaaaacttt agatgaaatt cttcaggaaa agaaacgaag gaaggaacaa gaggagaaag
     3721 cagagataaa acgcttaaaa aattctgatg accgggattc caagcgggat tcccttgagg
     3781 agggggagct gagagatcac tgcatggaga tcacaataag gaactccccg tatagaagag
     3841 aagactcaat ggaagacaga ggagaagaag atgattcttt ggccatcaaa ccaccccagc
     3901 aaatgtctcg gaaagaaaaa gttcatcaca gaaaagatga aaagagaaaa gaaaaatgga
     3961 agcatgctag agtgaaagaa agagagcacg aacgtcggaa acgacatcga gaagaacagg
     4021 ataaagctcg ccgggaatgg gaaagacaga agagaaggga aatggcaagg gagcattcca
     4081 ggagagaaag ggaccgcttg gagcagttag aaaggaagcg ggagcgggag cgcaagatgc
     4141 gggagcagca gaaggagcag cgggagcaga aggagcgcga gcggcgggcg gaggagcggc
     4201 gcaaggagcg ggaggcccgc agggaagtgt ctgcacatca ccgaacgatg agagaggact
     4261 acagcgacaa agtgaaagcc agccactgga gtcgcagccc gcctcggccg ccgcgggagc
     4321 ggttcgagtt gggagacggc cggaagccag gtgaggccag gccggcgcct gcgcagaagc
     4381 cagcacagtt aaaagaagag aaaatggaag aaagggacct gctgtccgac ttacaggaca
     4441 tcagcgacag cgagaggaag accagctcgg ccgagtcctc gtcagcggaa tcaggctcag
     4501 gttctgagga agaagaggag gaggaggaag aggaggagga ggaagggagc accagtgaag
     4561 aatcagagga ggaagaggag gaagaggaag aggaggagga ggagaccggc agcaactctg
     4621 aggaggcatc agagcagtct gccgaagaag taagtgagga agaaatgagt gaagatgaag
     4681 aacgagaaaa tgaaaaccac ctcttggttg ttccagagtc acggttcgac cgagattccg
     4741 gggagagtga agaagcagag gaagaagtgg gtgagggaac gccgcagagc agcgccctga
     4801 cagagggcga ctatgtgccc gactcccctg ccctgttgcc catcgagctc aagcaggagc
     4861 tgcccaagta cctgccggcc ctgcagggct gccggagcgt cgaggagttc cagtgcctga
     4921 acaggatcga ggagggcacc tatggagtgg tctacagagc aaaagacaag aaaacagatg
     4981 aaattgtggc tctaaagcgg ctgaagatgg agaaggagaa ggagggcttc ccgatcacgt
     5041 ccctgaggga gatcaacacc atcctcaagg cccagcatcc caacattgtc accgttagag
     5101 agattgtggt gggcagcaac atggacaaga tctacatcgt gatgaactac gtggagcacg
     5161 acctcaagag cctgatggag accatgaaac agcccttcct gccaggggag gtgaagaccc
     5221 tgatgatcca gctgctgcgg ggggtgaaac acctgcacga caactggatc ctgcaccgtg
     5281 acctcaagac gtccaacctg ctgctgagcc acgccggcat cctcaaggtg agcgattttg
     5341 ggctggcgcg ggagtacgga tcccctctga aggcctacac cccggtcgtg gtgacccagt
     5401 ggtaccgcgc cccagagctg ctgcttggtg ccaaggaata ctccacggcc gtggacatgt
     5461 ggtcagtggg ctgcatcttc ggggagctgc tgactcagaa gcctctgttc cccgggaatt
     5521 cggaaatcga tcagatcaac aaagtgttca aggagctggg gacccccagt gagaaaatct
     5581 ggcccggcta cagtgagctc ccagtagtca aaaagatgac cttcagcgag cacccctaca
     5641 acaacctccg caagcgcttc ggggctctgc tctcagacca gggcttcgac ctcatgaaca
     5701 agttcctgac ctacttcccc gggaggagga tcagcgctga ggacggcctc aagcatgagt
     5761 atttccgcga gacccccctc cccatcgacc cctccatgtt ccccacgtgg cccgccaaga
     5821 gcgagcagca gcgtgtgaag cggggcacca gcccgaggcc ccctgaggga ggcctgggct
     5881 acagccagct gggtgacgac gacctgaagg agacgggctt ccaccttacc accacgaacc
     5941 agggggcctc tgccgcgggc cccggcttca gcctcaagtt ctgaacccag ctttcttgta
     6001 caaagtggtg ataatcgaat tccgataatc aacctctgga ttacaaaatt tgtgaaagat
     6061 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6121 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6181 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6241 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6301 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     6361 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     6421 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     6481 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     6541 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     6601 gggccgcctc cccgcatcgg gaattcccgc ggttcgaatt ctaccgggta ggggaggcgc
     6661 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca
     6721 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt
     6781 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg
     6841 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg
     6901 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat
     6961 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg
     7021 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat
     7081 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc
     7141 tttcgacctc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     7201 acaaggtgag gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg
     7261 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     7321 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     7381 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     7441 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     7501 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     7561 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag
     7621 tacaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7681 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7741 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     7801 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     7861 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     7921 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     7981 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     8041 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     8101 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     8161 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     8221 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     8281 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     8341 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     8401 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     8461 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     8521 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     8581 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     8641 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     8701 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     8761 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     8821 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     8881 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     8941 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     9001 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9061 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9121 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9181 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9241 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9301 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9361 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     9421 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     9481 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     9541 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9601 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     9661 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9721 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9781 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9841 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9901 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9961 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10021 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10081 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
