LOCUS       sequence-474484-        7409 bp    DNA     circular SYN 04-JUN-2025
DEFINITION  AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Addgene Research Program
  TITLE     Addgene AAV RepCap Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 4, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV2/6 v1
FEATURES             Location/Qualifiers
     source          1..7409
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3726..3737
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(4040..4056)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4040..4056)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4272..4292)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4272..4290)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4311..4327)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4311..4327)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4324..4346)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4335..4351
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4359..4365,4366..4383,4384..4389))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4404..4425
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4542..4559)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4713..5301)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4793..4812)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5472..6263,6264..6332))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6095..6114
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6333..6437)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6463..6918)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6600..6621)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6812..6831
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7045..7067
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7059..7076
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7060..7076
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7086..7105
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7086..7104
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7129..7145
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7130..7146
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagtaaggcc ccggaggctc ttttctttgt gcaatttgag aagggagaga gctacttcca
       61 catgcacgtg ctcgtggaaa ccaccggggt gaaatccatg gttttgggac gtttcctgag
      121 tcagattcgc gaaaaactga ttcagagaat ttaccgcggg atcgagccga ctttgccaaa
      181 ctggttcgcg gtcacaaaga ccagaaatgg cgccggaggc gggaacaagg tggtggatga
      241 gtgctacatc cccaattact tgctccccaa aacccagcct gagctccagt gggcgtggac
      301 taatatggaa cagtatttaa gcgcctgttt gaatctcacg gagcgtaaac ggttggtggc
      361 gcagcatctg acgcacgtgt cgcagacgca ggagcagaac aaagagaatc agaatcccaa
      421 ttctgatgcg ccggtgatca gatcaaaaac ttcagccagg tacatggagc tggtcgggtg
      481 gctcgtggac aaggggatta cctcggagaa gcagtggatc caggaggacc aggcctcata
      541 catctccttc aatgcggcct ccaactcgcg gtcccaaatc aaggctgcct tggacaatgc
      601 gggaaagatt atgagcctga ctaaaaccgc ccccgactac ctggtgggcc agcagcccgt
      661 ggaggacatt tccagcaatc ggatttataa aattttggaa ctaaacgggt acgatcccca
      721 atatgcggct tccgtctttc tgggatgggc cacgaaaaag ttcggcaaga ggaacaccat
      781 ctggctgttt gggcctgcaa ctaccgggaa gaccaacatc gcggaggcca tagcccacac
      841 tgtgcccttc tacgggtgcg taaactggac caatgagaac tttcccttca acgactgtgt
      901 cgacaagatg gtgatctggt gggaggaggg gaagatgacc gccaaggtcg tggagtcggc
      961 caaagccatt ctcggaggaa gcaaggtgcg cgtggaccag aaatgcaagt cctcggccca
     1021 gatagacccg actcccgtga tcgtcacctc caacaccaac atgtgcgccg tgattgacgg
     1081 gaactcaacg accttcgaac accagcagcc gttgcaagac cggatgttca aatttgaact
     1141 cacccgccgt ctggatcatg actttgggaa ggtcaccaag caggaagtca aagacttttt
     1201 ccggtgggca aaggatcacg tggttgaggt ggagcatgaa ttctacgtca aaaagggtgg
     1261 agccaagaaa agacccgccc ccagtgacgc agatataagt gagcccaaac gggtgcgcga
     1321 gtcagttgcg cagccatcga cgtcagacgc ggaagcttcg atcaactacg cagacaggta
     1381 ccaaaacaaa tgttctcgtc acgtgggcat gaatctgatg ctgtttccct gcagacaatg
     1441 cgagagaatg aatcagaatt caaatatctg cttcactcac ggacagaaag actgtttaga
     1501 gtgctttccc gtgtcagaat ctcaacccgt ttctgtcgtc aaaaaggcgt atcagaaact
     1561 gtgctacatt catcatatca tgggaaaggt gccagacgct tgcactgcct gcgatctggt
     1621 caatgtggat ttggatgact gcatctttga acaataaatg atttaaatca ggtatggctg
     1681 ccgatggtta tcttccagat tggctcgagg acaacctctc tgagggcatt cgcgagtggt
     1741 gggacttgaa acctggagcc ccgaaaccca aagccaacca gcaaaagcag gacgacggcc
     1801 ggggtctggt gcttcctggc tacaagtacc tcggaccctt caacggactc gacaaggggg
     1861 agcccgtcaa cgcggcggat gcagcggccc tcgagcacga caaggcctac gaccagcagc
     1921 tcaaagcggg tgacaatccg tacctgcggt ataaccacgc cgacgccgag tttcaggagc
     1981 gtctgcaaga agatacgtct tttgggggca acctcgggcg agcagtcttc caggccaaga
     2041 agagggttct cgaacctttt ggtctggttg aggaaggtgc taagacggct cctggaaaga
     2101 aacgtccggt agagcagtcg ccacaagagc cagactcctc ctcgggcatt ggcaagacag
     2161 gccagcagcc cgctaaaaag agactcaatt ttggtcagac tggcgactca gagtcagtcc
     2221 ccgacccaca acctctcgga gaacctccag caacccccgc tgctgtggga cctactacaa
     2281 tggcttcagg cggtggcgca ccaatggcag acaataacga aggcgccgac ggagtgggta
     2341 atgcctcagg aaattggcat tgcgattcca catggctggg cgacagagtc atcaccacca
     2401 gcacccgaac atgggccttg cccacctata acaaccacct ctacaagcaa atctccagtg
     2461 cttcaacggg ggccagcaac gacaaccact acttcggcta cagcaccccc tgggggtatt
     2521 ttgatttcaa cagattccac tgccatttct caccacgtga ctggcagcga ctcatcaaca
     2581 acaattgggg attccggccc aagagactca acttcaagct cttcaacatc caagtcaagg
     2641 aggtcacgac gaatgatggc gtcacgacca tcgctaataa ccttaccagc acggttcaag
     2701 tcttctcgga ctcggagtac cagttgccgt acgtcctcgg ctctgcgcac cagggctgcc
     2761 tccctccgtt cccggcggac gtgttcatga ttccgcagta cggctaccta acgctcaaca
     2821 atggcagcca ggcagtggga cggtcatcct tttactgcct ggaatatttc ccatcgcaga
     2881 tgctgagaac gggcaataac tttaccttca gctacacctt cgaggacgtg cctttccaca
     2941 gcagctacgc gcacagccag agcctggacc ggctgatgaa tcctctcatc gaccagtacc
     3001 tgtattacct gaacagaact cagaatcagt ccggaagtgc ccaaaacaag gacttgctgt
     3061 ttagccgggg gtctccagct ggcatgtctg ttcagcccaa aaactggcta cctggaccct
     3121 gttaccggca gcagcgcgtt tctaaaacaa aaacagacaa caacaacagc aactttacct
     3181 ggactggtgc ttcaaaatat aaccttaatg ggcgtgaatc tataatcaac cctggcactg
     3241 ctatggcctc acacaaagac gacaaagaca agttctttcc catgagcggt gtcatgattt
     3301 ttggaaagga gagcgccgga gcttcaaaca ctgcattgga caatgtcatg atcacagacg
     3361 aagaggaaat caaagccact aaccccgtgg ccaccgaaag atttgggact gtggcagtca
     3421 atctccagag cagcagcaca gaccctgcga ccggagatgt gcatgttatg ggagccttac
     3481 ctggaatggt gtggcaagac agagacgtat acctgcaggg tcctatttgg gccaaaattc
     3541 ctcacacgga tggacacttt cacccgtctc ctctcatggg cggctttgga cttaagcacc
     3601 cgcctcctca gatcctcatc aaaaacacgc ctgttcctgc gaatcctccg gcagagtttt
     3661 cggctacaaa gtttgcttca ttcatcaccc agtattccac aggacaagtg agcgtggaga
     3721 ttgaatggga gctgcagaaa gaaaacagca aacgctggaa tcccgaagtg cagtatacat
     3781 ctaactatgc aaaatctgcc aacgttgatt tcactgtgga caacaatgga ctttatactg
     3841 agcctcgccc cattggcacc cgttacctca cccgtcccct gtaattacgt gttaatcaat
     3901 aaaccggttg attcgtttca gttgaacttt ggtctcctgt ccttcttatc ttatcggtta
     3961 ccatggttat agcttacaca ttaactgctt ggttgcgctt cgcgataaaa gacttacgtc
     4021 atcgggttac ccctaggggg atccactagt tctagaggtc ctgtattaga ggtcacgtga
     4081 gtgttttgcg acattttgcg acaccatgtg gtcacgctgg gtatttaagc ccgagtgagc
     4141 acgcagggtc tccattttga agcgggaggt ttgaacgcgc agccgccaag ccgaattctg
     4201 cagatatcca tcacactggc ggccgctcga ctagagcggc cgccaccgcg gtggagctcc
     4261 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     4321 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4381 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4441 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4501 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4561 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4621 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5461 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5521 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5581 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5641 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5701 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5761 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5821 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5881 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5941 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6001 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6061 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6121 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6181 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6241 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6301 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6361 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6421 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     6481 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     6541 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     6601 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     6661 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     6721 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     6781 tgggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     6841 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     6901 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6961 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     7021 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     7081 gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcgacg
     7141 gtatcggggg agctcgcagg gtctccattt tgaagcggga ggtttgaacg cgcagccgcc
     7201 atgccggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc
     7261 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat
     7321 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctgcag
     7381 cgcgactttc tgacggaatg gcgccgtgt
//
