LOCUS       sequence-474740-       11801 bp    DNA     circular SYN 05-JUN-2025
DEFINITION  Expresses TRF1-PYL1 for recruiting RNA to the nuclear stress body 
            via CRISPR-TO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11801)
  TITLE     Qi lab plasmids
REFERENCE   2  (bases 1 to 11801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSLQ14806
FEATURES             Location/Qualifiers
     source          1..11801
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(158..176)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(159..176)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(466..485)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(699..718)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     818..840
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(878..896)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        964..1068
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1069..1137,1138..1929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1287..1306)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2100..2688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2589..2608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2842..2859
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2929..2949)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2973..3183
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        2995..3191
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3042..3177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4325..4390
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4520..4540
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4965..5099
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5002..5021)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5056..5075
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5267..5900
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5947..6072
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6569..6802
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6987..7031
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7316..7433
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7498..7997
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7533..7556)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7930..7949
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9289..9660
                     /codon_start=1
                     /product="C-terminal activation domain from the human heat 
                     shock transcription factor HSF1"
                     /label=HSF1
                     /translation="GFSVDTSALLDLFSPSVTVPDMSLPDLDSSLASIQELLSPQEPPR
                     PPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPVLFELGEGSYFSEGDGFAED
                     PTISLLTGSEPPKAKDPTVS"
     CDS             9709..10404
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     misc_feature    11003..11591
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11056..11076)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11474..11485)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11594..11610)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11595..11611)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             join(11701..11801,1..133)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
       61 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
      121 aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta
      181 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca
      241 agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg
      301 ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc
      361 cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac
      421 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
      481 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
      541 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
      601 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
      661 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca
      721 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
      781 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
      841 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
      901 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      961 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     1021 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     1081 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     1141 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     1201 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     1261 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     1321 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     1381 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     1441 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1501 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1561 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1621 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1681 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1741 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1801 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1861 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1921 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1981 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     2041 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     2101 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2161 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     2221 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     2281 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     2341 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     2401 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     2461 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     2521 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2581 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2641 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2701 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2761 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2821 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     2881 cgcaaaccgc ctctccccgc gcgtcggccg attcattaat gcagctgtgg aatgtgtgtc
     2941 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3001 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3061 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3121 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3181 gcttttgcaa aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt
     3241 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     3301 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     3361 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     3421 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     3481 ggcattattg ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg
     3541 gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc
     3601 ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg
     3661 tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca
     3721 ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc
     3781 aagatacctg gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg
     3841 gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg
     3901 ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc
     3961 gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt
     4021 aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg
     4081 tatcgcatga ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc
     4141 gacgtccagg cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat
     4201 acggtgattg gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa
     4261 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     4321 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     4381 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     4441 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     4501 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     4561 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     4621 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     4681 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     4741 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     4801 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     4861 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     4921 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     4981 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5041 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
     5101 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca
     5161 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat
     5221 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt
     5281 cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     5341 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     5401 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     5461 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     5521 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     5581 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     5641 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     5701 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     5761 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     5821 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     5881 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     5941 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     6001 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     6061 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     6121 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     6181 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     6241 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     6301 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     6361 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc
     6421 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     6481 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     6541 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     6601 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     6661 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     6721 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     6781 acctaaagga tcaacagctc ctggggattt ggtgttgctc tggaaaactc atttgcacca
     6841 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     6901 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     6961 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7021 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7081 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7141 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     7201 gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca
     7261 ttcgattagt gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta
     7321 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     7381 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     7441 attacaggga cagcagagat ccagtttgga tcgataagct tgatatcgaa ttctaccggg
     7501 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     7561 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     7621 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     7681 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     7741 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     7801 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     7861 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     7921 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     7981 catctccggg cctttcgacc tgcagcccaa gcttacgcgt ctgcaggata tcaagcttgc
     8041 ggtacgactg agtcgcggcc gcgccaccgt accatggatc tgcccgtggg ccccggcgcg
     8101 gcggggccca gcaacgtccc ggccttcctg accaagctgt ggaccctcgt gagcgacccg
     8161 gacaccgacg cgctcatctg ctggagcccg agcgggaaca gcttccacgt gttcgaccag
     8221 ggccagtttg ccaaggaggt gctgcccaag tacttcaagc acaacaacat ggccagcttc
     8281 gtgcggcagc tcaacatgta tggcttccgg aaagtggtcc acatcgagca gggcggcctg
     8341 gtcaagccag agagagacga cacggagttc cagcacccat gcttcctgcg tggccaggag
     8401 cagctccttg agaacatcaa gaggaaagtg accagtgtgt ccaccctgaa gagtgaagac
     8461 ataaagatcc gccaggacag cgtcaccaag ctgctgacgg acgtgcagct gatgaagggg
     8521 aagcaggagt gcatggactc caagctcctg gccatgaagc atgagaatga ggctctgtgg
     8581 cgggaggtgg ccagccttcg gcagaagcat gcccagcaac agaaagtcgt caacaagctc
     8641 attcagttcc tgatctcact ggtgcagtca aaccggatcc tgggggtgaa gagaaagatc
     8701 cccctgatgc tgaacgacag tggctcagca cattccatgc ccaagtatag ccggcagttc
     8761 tccctggagc acgtccacgg ctcgggcccc tactcggccc cctccccagc ctacagcagc
     8821 tccagcctct acgcccctga tgctgtggcc agctctggac ccatcatctc cgacatcacc
     8881 gagctggctc ctgccagccc catggcctcc cccggcggga gcatagacga gaggccccta
     8941 tccagcagcc ccctggtgcg tgtcaaggag gagcccccca gcccgcctca gagcccccgg
     9001 gtagaggagg cgagtcccgg gcgcccatct tccgtggaca ccctcttgtc cccgaccgcc
     9061 ctcattgact ccatcctgcg ggagagtgaa cctgcccccg cctccgtcac agccctcacg
     9121 gacgccaggg gccacacgga caccgagggc cggcctccct cccccccgcc cacctccacc
     9181 cctgaaaagt gcctcagcgt agcctgcctg gacaagaatg agctcagtga ccacttggat
     9241 gctatggact ccaacctgga taacctgcag accatgctga gcagccacgg cttcagcgtg
     9301 gacaccagtg ccctgctgga cctgttcagc ccctcggtga ccgtgcccga catgagcctg
     9361 cctgaccttg acagcagcct ggccagtatc caagagctcc tgtctcccca ggagcccccc
     9421 aggcctcccg aggcagagaa cagcagcccg gattcaggga agcagctggt gcactacaca
     9481 gcgcagccgc tgttcctgct ggaccccggc tccgtggaca ccgggagcaa cgacctgccg
     9541 gtgctgtttg agctgggaga gggctcctac ttctccgaag gggacggctt cgccgaggac
     9601 cccaccatct ccctgctgac aggctcggag cctcccaaag ccaaggaccc cactgtctcc
     9661 ggcggaccgg gatcaggaag cgtggatcca ggtggaggtg gaagcggtag cgagctgatt
     9721 aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag
     9781 tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg
     9841 gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc
     9901 agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct
     9961 gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc
    10021 caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac
    10081 ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag
    10141 acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg
    10201 ggcgggagcc atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag
    10261 aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc
    10321 aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct
    10381 agcaaactgg ggcacaagct taatggatca ggaagcggtg ggggcgcgcc aactcaagac
    10441 gaattcaccc aactctccca atcaatcgcc gagttccaca cgtaccaact cggtaacggc
    10501 cgttgctcat ctctcctagc tcagcgaatc cacgcgccgc cggaaacagt atggtccgtg
    10561 gtgagacgtt tcgataggcc acagatttac aaacacttca tcaaaagctg taacgtgagt
    10621 gaagatttcg agatgcgagt gggatgcacg cgcgacgtga acgtgataag tggattaccg
    10681 gcgaatacgt ctcgagagag attagatctg ttggacgatg atcggagagt gactgggttt
    10741 agtataaccg gtggtgaaca taggctgagg aattataaat cggttacgac ggttcataga
    10801 tttgagaaag aagaagaaga agaaaggatc tggaccgttg ttttggaatc ttatgttgtt
    10861 gatgtaccgg aaggtaattc ggaggaagat acgagattgt ttgctgatac ggttattaga
    10921 ttgaatcttc agaaacttgc ttcgatcact gaagctatga actgacctgc aggcatgcaa
    10981 gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
    11041 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
    11101 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
    11161 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
    11221 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
    11281 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
    11341 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
    11401 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
    11461 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
    11521 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
    11581 cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg gtacctttaa
    11641 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
    11701 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
    11761 tctggttaga ccagatctga gcctgggagc tctctggcta a
//
