LOCUS       sequence-474754-       11893 bp    DNA     circular SYN 05-JUN-2025
DEFINITION  Expresses PYL1-KIFC1* for the retrograde transport of RNA along 
            microtubules via CRISPR-TO.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11893)
  TITLE     Qi lab plasmids
REFERENCE   2  (bases 1 to 11893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 5, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSLQ10622
FEATURES             Location/Qualifiers
     source          1..11893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    723..857
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(747..766)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     801..820
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1282..1302)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          1432..1497
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(2631..2960)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2645..2780
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2945..2965
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     primer_bind     complement(3035..3052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3206..3794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3286..3305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3965..4756,4757..4825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4588..4607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4826..4930)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4998..5016
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(5054..5076)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     5176..5195
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5409..5428
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5718..5736
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5718..5735
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             5761..5994
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     6084..6100
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6085..6101
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6104..6692
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             6210..6221
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     6619..6639
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8407..9102)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     promoter        complement(9718..10217)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9766..9785)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     10159..10182
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    10282..10399
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(10684..10728)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10913..11146
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11643..11768
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(11815..11893,1..555)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc
       61 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagg caaaaagcag
      121 ctgcttatat gcaggatctg agggctcgcc actccccagt cccgcccagg ccacgcctcc
      181 ctggaaagtc cccagcggaa agtcccttgt agcaagctcg atatcagcag ttcttgaagt
      241 actccggatg cagctctcgg gccacgtgat gaaatgctag gcggctgtca aacctccact
      301 ctaacacttc tctctccggg tcatccatcc catgcaggct cacagggtgt aacaagctgg
      361 tgttctctcc tttattggcc tcttctacct tatctggctc aactggtact agcttgtagc
      421 accatccaaa ggtcagtgga tatctgaccc ctggccctgg tgtgtagttc tgctaatcag
      481 ggaagtagcc ttgtgtgtgg tagatccaca gatcaaggat atcttgtctt ctttgggagt
      541 gaattagccc ttccaactac taagtttgta gtacatattt aacaaataca atttctttaa
      601 aatgaaaata attcagagga atcacaggtt tagagtaaat gaaaccacag gtaattggca
      661 gtggtaatag ggtatggggt gggaagtttg ggatgatttt ggttagcttg agttatccag
      721 ttgatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
      781 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
      841 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
      901 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg
      961 atctctagtc aaggcactat acatcaaata ttccttatta acccctttac aaattaaaaa
     1021 gctaaaggta cacaattttt gagcatagtt attaatagca gacactctat gcctgtgtgg
     1081 agtaagaaaa aacagtatgt tatgattata actgttatgc ctacttataa aggttacaga
     1141 atatttttcc ataattttct tgtatagcag tgcagctttt tcctttgtgg tgtaaatagc
     1201 aaagcaagca agagttctat tactaaacac agcatgactc aaaaaactta gcaattctga
     1261 aggaaagtcc ttggggtctt ctacctttct cttctttttt ggaggagtag aatgttgaga
     1321 gtcagcagta gcctcatcat cactagatgg catttcttct gagcaaaaca ggttttcctc
     1381 attaaaggca ttccaccact gctcccattc atcagttcca taggttggaa tctaaaatac
     1441 acaaacaatt agaatcagta gtttaacaca ttatacactt aaaaatttta tatttacctt
     1501 agagctttaa atctctgtag gtagtttgtc caattatgtc acaccacaga agtaaggttc
     1561 cttcacaaag atccggggcc cactcataaa tccagttgcc gccacggtag ccaatcaccg
     1621 tatcgtataa atcatcgtcg gtacgttcgg catcgctcat cacaatacgt gcctggacgt
     1681 cgaggatttc gcgtgggtca atgccgcgcc agatccacat cagacggtta atcatgcgat
     1741 accagtgagg gatggtttta ccatcaaggg ccgactgcac aggcggttgt gcgccgtgat
     1801 taaagcggcg gactagcgtc gaggtttcag gatgtttaaa gcggggtttg aacagggttt
     1861 cgctcaggtt tgcctgtgtc atggatgcag cctccagaat acttactgga aactattgta
     1921 acccgcctga agttaaaaag aacaacgccc ggcagtgcca ggcgttgaaa agattagcga
     1981 ccggagattg gcgggacgaa tacgacgccc atatcccacg gctgttcaat ccaggtatct
     2041 tgcgggatat caacaacata gtcatcaacc agcggacgac cagccggttt tgcgaagatg
     2101 gtgacaaagt gcgcttttgg atacatttca cgaatcgcaa ccgcagtacc accggtatcc
     2161 accaggtcat caataacgat gaagccttcg ccatcgcctt ctgcgcgttt cagcacttta
     2221 agctcgcgct ggttgtcgtg atcgtagctg gaaatacaaa cggtatcgac atgacgaata
     2281 cccagttcac gcgccagtaa cgcacccggt acagaccgcc acggcttacg gcaataatgc
     2341 ctttccattg ttcagaaggc atcagtcggc ttgcgagttt acgtgcatgg atctgcaaca
     2401 tgtcccaggt gacgatgtat ttttcgctca tgtgaagtgt cccagcctgt ttatctacgg
     2461 cttaaaaagt gttcgagggg aaaataggtt gcgcgagatt atagagatct ggcgcactaa
     2521 aaaccagtat ttcacatgag tccgcgtctt tttacgcact gcctctccct gacgcgggat
     2581 aaagtggtat tctcaaacat atctcgcaag cctgtcttgt gtccaagctt tttgcaaaag
     2641 cctaggcctc caaaaaagcc tcctcactac ttctggaata gctcagaggc cgaggcggcc
     2701 tcggcctctg cataaataaa aaaaattagt cagccatggg gcggagaatg ggcggaactg
     2761 ggcggagtta ggggcgggat gggcggagtt aggggcggga ctatggttgc tgactaattg
     2821 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acacctggtt
     2881 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
     2941 ccacacccta actgacacac attccacagc tgcattaatg aatcggccga cgcgcgggga
     3001 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     3061 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     3121 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     3181 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     3241 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     3301 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     3361 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     3421 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3481 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3541 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3601 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     3661 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3721 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3781 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3841 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3901 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3961 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     4021 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     4081 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     4141 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     4201 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     4261 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     4321 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     4381 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     4441 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     4501 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     4561 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4621 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4681 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4741 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4801 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     4861 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     4921 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     4981 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     5041 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5101 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5161 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat tcgacgctct
     5221 cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc
     5281 gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg
     5341 cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct
     5401 tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg
     5461 ccggccacga tgcgtccggc gtagaggatc tggctagcga tgaccctgct gattggttcg
     5521 ctgaccattt ccgggtgcgg gacggcgtta ccagaaactc agaaggttcg tccaaccaaa
     5581 ccgactctga cggcagttta cgagagagat gatagggtct gcttcagtaa gccagatgct
     5641 acacaattag gcttgtacat attgtcgtta gaacgcggct acaattaata cataacctta
     5701 tgtatcatac acatacgatt taggtgacac tatagaatac acggaattaa ttctagatgc
     5761 tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac
     5821 acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca
     5881 gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc
     5941 cagtacaagc aaaaagcaga tcttgtcttc gttgggagtg aattagccct tccagtcccc
     6001 ccttttcttt taaaaagtgg ctaagatcta cagctgcctt gtaagtcatt ggtcttaaag
     6061 gtaccgagct cgaattaatt ccctcgaggt cgacggtatc gatgcgggga ggcggcccaa
     6121 agggagatcc gactcgtctg agggcgaagg cgaagacgcg gaagaggccg cagagccggc
     6181 agcaggccgc gggaaggaag gtccgctgga ttgagggccg aagggacgta gcagaaggac
     6241 gtcccgcgca gaatccaggt ggcaacacag gcgagcagcc aaggaaagga cgatgatttc
     6301 cccgacaaca ccacggaatt gtcagtgccc aacagccgag cccctgtcca gcagcgggca
     6361 aggcaggcgg cgatgagttc cgccgtggca atagggaggg ggaaagcgaa agtcccggaa
     6421 aggagctgac aggtggtggc aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg
     6481 cacaccacgc cacgttgcct gacaacgggc cacaactcct cataaagaga cagcaaccag
     6541 gatttataca aggaggagaa aatgaaagcc atacgggaag caatagcatg atacaaaggc
     6601 attaaagcag cgtatccaca tagcgtaaaa ggagcaacat agttaagaat accagtcaat
     6661 ctttcacaaa ttttgtaatc cagaggttga ttatcgataa gcttgatatc aagcttgcat
     6721 gcctgcaggt cacttcctgt tggcctgagc agtaccaata acacactggt tcaccttgga
     6781 ggcaaagcgt agagagttga gggactcgga gacgttctct tccagtggag aaatgttcac
     6841 aaacatgagc atcttagcac taccacccag agagttctgc agcaggtagg tcagtttgct
     6901 gttccggtaa ggcacgtggg actccttgtt gctcagggcc atgataacca gccccagcgt
     6961 ggacaggctg ctgttaatgg cctgtgtttc ccgaaggcgt tcccgctccc cggggccgag
     7021 ggctaagccg gggtcaagtc gctcactccc ggccaggtcc acaagactga ggggggcccc
     7081 acactgcagg cctcggctgg agtgctcccc agaaatctgt agctggaata cactgtggct
     7141 gcgtgatgac cgttcattct gggctgtgcg ggccacagcc cgattctggc gggccagatg
     7201 aagcagggcg tccacttctt tctcacagga gacagggaca tatcgagcat tggtgacagt
     7261 gagctcctca ctccctggcc ctgcacggcg aatctcacac tcgccccctt gacccttccg
     7321 ggttccagtg gccagcaggt cccggacagt ctcattgtag atctctacgt agcttgctac
     7381 aaagctgtag gtccagccct gaccactcag ctcctgagcc acagagaaga ggtgccgcag
     7441 ggcccgaggg atcagcccct ccaactgggg gtctccccca ggcccaccct ccattgtgaa
     7501 ggtcttgcca ctgcctgtct ggccataggc aaagatgcat actggatagc catccagggc
     7561 tgactggaca agcatggcaa tctcttcaaa cacttcatcc tgtccacttc ctggtgggaa
     7621 tacccggtca aaggaaaaat catggcgagt tgggggagct ggtgccccac tcagggtccc
     7681 acgccgctcg tcagaccggg agaggctaag gcgggttgga ggatcagagg gcccaccagg
     7741 gccagaggga aacaggagga ggccaggggg tggagtgggc tcccccggca ggacagggcg
     7801 gacccggcag aatacacgga tgttgccctt gagttcctgc agctggttgt gcagtcgccg
     7861 gcgctccatt tctagcccat gaagacgttc ttcccgctca gtcagtaagg ctgcctgggc
     7921 cacagtctcc tgccgcagag atgccacctc tgcttggctg cttgacaggg ctgcttctga
     7981 tgtctgcagc ctcctctcct tctcctctag ttgggacatc agtcccctcc gttcttcctg
     8041 caattccacc tgttttttct gaagctcttg caccaagccc tcctgcgtgc tcagccgctc
     8101 ttccagctcc aggacacaag cacgcaagtt cttcagctcc tgttggccct gctcagcctg
     8161 ggcctgtacc ttggctaaat gcccctccag tgttgtgcgc tctgtcccca gggccttgac
     8221 ctgctgctgg gcatctctga gctggtcctg aagctgctgg ttctcttggt ccaacgtttg
     8281 agtcctctca cggcaccgtt ttagttctgc atttaggtca cataactgac cctttaagtc
     8341 ccaggctgga cgtttgctgg gtttcttccc tcctgccatg ctaccgctgc cgctaccacc
     8401 ggtccgatta agcttgtgcc ccagtttgct agggaggtcg cagtatctgg ccactgccac
     8461 ctcgtgctgc tcgacgtagg tctcgttgtt ggcctccttg attctttcca gtctgtagtc
     8521 cacatagtag acgccaggca tcttgaggtt cttagcgggt ttcttggatc tatatgtggt
     8581 cttgatgttt gcgatcagat ggctcccgcc cacgagcttc agggccatgt cgtttctgcc
     8641 ttccaggccg ccgtcagcgg ggtacagcgt ctcggtgaag gcctcccagc cgagtgtttt
     8701 cttctgcatc acagggccgt tggatgtgaa gttcacccct ctgatcttga cgttgtagat
     8761 gaggcagccg tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta
     8821 tgtggtgact ctctcccatg tgaagccctc agggaaggac tgcttgaaga agtcggggat
     8881 gccctgggtg tggttgatga aggtcttgct gccgtagagg aagctagtag ccaggatgtc
     8941 gaaggcgaag gggagagggc cgccctcgac caccttgatt ctcatggtct gggtgccctc
     9001 gtagggcttg ccttcgccct cggatgtgca cttgaagtga tggttgtcca cggtgccctc
     9061 catgtacagc ttcatgtgca tgttctcctt aatcagctcg ctcggatcca cgttcatagc
     9121 ttcagtgatc gaagcaagtt tctgaagatt caatctaata accgtatcag caaacaatct
     9181 cgtatcttcc tccgaattac cttccggtac atcaacaaca taagattcca aaacaacggt
     9241 ccagatcctt tcttcttctt cttctttctc aaatctatga accgtcgtaa ccgatttata
     9301 attcctcagc ctatgttcac caccggttat actaaaccca gtcactctcc gatcatcgtc
     9361 caacagatct aatctctctc gagacgtatt cgccggtaat ccacttatca cgttcacgtc
     9421 gcgcgtgcat cccactcgca tctcgaaatc ttcactcacg ttacagcttt tgatgaagtg
     9481 tttgtaaatc tgtggcctat cgaaacgtct caccacggac catactgttt ccggcggcgc
     9541 gtggattcgc tgagctagga gagatgagca acggccgtta ccgagttggt acgtgtggaa
     9601 ctcggcgatt gattgggaga gttgggtgaa ttcgtcttga gttggcgcgc ccccacccat
     9661 gactcagtcg taccgcaagc ttgatatcct gcagacgcgt aagcttgggc tgcaggtcga
     9721 aaggcccgga gatgaggaag aggagaacag cgcggcagac gtgcgctttt gaagcgtgca
     9781 gaatgccggg cctccggagg accttcgggc gcccgccccg cccctgagcc cgcccctgag
     9841 cccgcccccg gacccacccc ttcccagcct ctgagcccag aaagcgaagg agcaaagctg
     9901 ctattggccg ctgccccaaa ggcctacccg cttccattgc tcagcggtgc tgtccatctg
     9961 cacgagacta gtgagacgtg ctacttccat ttgtcacgtc ctgcacgacg cgagctgcgg
    10021 ggcggggggg aacttcctga ctaggggagg agtagaaggt ggcgcgaagg ggccaccaaa
    10081 gaacggagcc ggttggcgcc taccggtgga tgtggaatgt gtgcgaggcc agaggccact
    10141 tgtgtagcgc caagtgccca gcggggctgc taaagcgcat gctccagact gccttgggaa
    10201 aagcgcctcc cctacccggt agaattcgat atcaagctta tcgatccaaa ctggatctct
    10261 gctgtccctg taataaaccc gaaaattttg aatttttgta atttgttttt gtaattcttt
    10321 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
    10381 cccccctttt cttttaaaat tgtggatgaa tactgccatt tggcgatacc gtcgagatcc
    10441 gttcactaat cgaatggatc tgtctctgtc tctctctcca ccttcttctt ctattccttc
    10501 gggcctgtcg ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
    10561 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
    10621 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
    10681 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
    10741 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
    10801 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
    10861 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
    10921 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
    10981 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
    11041 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
    11101 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
    11161 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
    11221 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctcc
    11281 aggtctgaag atcagcggcc ggccgcttgc tgtgcggtgg tcttactttt gttttgctct
    11341 tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa
    11401 tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat
    11461 ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag gccaggatta
    11521 actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt atattttttc
    11581 tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata
    11641 ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta
    11701 ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt
    11761 cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag
    11821 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
    11881 gacgggcaca cac
//
