LOCUS       sequence-475278-        5751 bp    DNA     circular SYN 30-JUL-2025
DEFINITION  Express IFNGR2-LgBiT Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5751)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 30, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: IFNGR2-LgBiT Vector
FEATURES             Location/Qualifiers
     source          1..5751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2694..2712)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    2749..2870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2760..2779)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2814..2833
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        3115..3472
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3115..3306
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3138..3158)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3323..3458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        3487..3534
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             3566..4360
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3620..3639)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4230..4249
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4424..4472
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4711..5299)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4791..4810)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    5415..5698
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatgcga ccgacgctgc
     1081 tgtggtcgct gctgctgctg ctcggagtct tcgccgccgc cgccgcggcc ccgccagacc
     1141 ctctttccca gctgcccgct cctcagcacc cgaagattcg cctgtacaac gcagagcagg
     1201 tcctgagttg ggagccagtg gccctgagca atagcacgag gcctgttgtc taccaagtgc
     1261 agtttaaata caccgacagt aaatggttca cggccgacat catgtccata ggggtgaatt
     1321 gtacacagat cacagcaaca gagtgtgact tcactgccgc cagtccctca gcaggcttcc
     1381 caatggattt caatgtcact ctacgccttc gagctgagct gggagcactc cattctgcct
     1441 gggtgacaat gccttggttt caacactatc ggaatgtgac tgtcgggcct ccagaaaaca
     1501 ttgaggtgac cccaggagaa ggctccctca tcatcaggtt ctcctctccc tttgacatcg
     1561 ctgatacctc cacggccttt ttttgttatt atgtccatta ctgggaaaaa ggaggaatcc
     1621 aacaggtcaa aggccctttc agaagcaact ccatttcatt ggataactta aaaccctcca
     1681 gagtgtactg tttacaagtc caggcacaac tgctttggaa caaaagtaac atctttagag
     1741 tcgggcattt aagcaacata tcttgctacg aaacaatggc agatgcctcc actgagcttc
     1801 agcaagtcat cctgatctcc gtgggaacat tttcgttgct gtcggtgctg gcaggagcct
     1861 gtttcttcct ggtcctgaaa tatagaggcc tgattaaata ctggtttcac actccaccaa
     1921 gcatcccatt acagatagaa gagtatttaa aagacccaac tcagcccatc ttagaggcct
     1981 tggacaagga cagctcacca aaggatgacg tctgggactc tgtgtccatt atctcgtttc
     2041 cggaaaagga gcaagaagat gttctccaaa cgcttgtttc tcaaggcagt tcaggtggtg
     2101 gcgggagcgg aggtggaggc tcgagcggtg tcttcacact cgaagatttc gttggggact
     2161 gggaacagac agccgcctac aacctggacc aagtccttga acagggaggt gtgtccagtt
     2221 tgctgcagaa tctcgccgtg tccgtaactc cgatccaaag gattgtccgg agcggtgaaa
     2281 atgccctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc gccgaccaaa
     2341 tggcccagat cgaagaggtg tttaaggtgg tgtaccctgt ggatgatcat cactttaagg
     2401 tgatcctgcc ctatggcaca ctggtaatcg acggggttac gccgaacatg ctgaactatt
     2461 tcggacggcc gtatgaaggc atcgccgtgt tcgacggcaa aaagatcact gtaacaggga
     2521 ccctgtggaa cggcaacaaa attatcgacg agcgcctgat cacccccgac ggctccatgc
     2581 tgttccgagt aaccatcaac agctaaggcc gcgactctag agtcgacctg caggcatgca
     2641 agctgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
     2701 gcaataacta gcataacccc ttggggcggc cgcttcgagc agacatgata agatacattg
     2761 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     2821 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     2881 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt ttaagcaagt
     2941 aaaacctcta caaatgtggt aaaatcgaat tttaacaaaa tattaacgct tacaatttcc
     3001 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgcggatctg
     3061 cgcagcacca tggcctgaaa taacctctga aagaggaact tggttaggta ccttctgagg
     3121 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     3181 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     3241 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     3301 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3421 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttaa
     3481 ttaactgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     3541 aggaactaaa cccaggaggc agatcatgat tgaacaagat ggattgcacg caggttctcc
     3601 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3661 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3721 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     3781 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     3841 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     3901 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     3961 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     4021 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4081 caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4141 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4201 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4261 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4321 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     4381 atgaccgacc aagcgacgcc caacctgcca tcacgatggc cgcaataaaa tatctttatt
     4441 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gcgataagga tcctctttgc
     4501 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     4561 tttctgcgga ctggctttct acccggtatc agctcactca aaggcggtaa tacggttatc
     4621 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     4681 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     4741 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     4801 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     4861 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     4921 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     4981 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5041 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5101 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     5161 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5221 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5281 cagaaaaaaa ggatttcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5341 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5401 gatcctttta tagtccggaa atacaggaac gcacgctgga tggcccttcg ctgggatggt
     5461 gaaaccatga aaaatggcag cttcagtgga ttaagtgggg gtaatgtggc ctgtaccctc
     5521 tggttgcata ggtattcata cggttaaaat ttatcaggcg cgattgcggc agtttttcgg
     5581 gtggtttgtt gccattttta cctgtctgct gccgtgatcg cgctgaacgc gttttagcgg
     5641 tgcgtacaat taagggatta tggtaaatcc acttactgtc tgccctcgta gccatcgaga
     5701 taaaccgcag tactccggcc acgatgcgtc cggcgtagag gatcgagatc t
//
