LOCUS       sequence-475554-       11201 bp    DNA     circular SYN 10-JUN-2025
DEFINITION  Express HA-tagged NUP98::RAP1GDS1 in dual promoter lentiviral 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11201)
  TITLE     Mullighan lab plasmids 2025
REFERENCE   2  (bases 1 to 11201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CCLMPC_HA-NUP98::RAP1GDS1
FEATURES             Location/Qualifiers
     source          1..11201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1446..1470)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        1499..1998
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1534..1557)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1931..1950
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      2019..2028
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2025..2735
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2170..2188)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2438..2457
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             2857..3090
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3162..3283
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3199..3218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3253..3272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3323..3458
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3478..3497)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3479..3497)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3507..3524)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3507..3523)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3516..3538)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3665..4120
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3752..3771)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3962..3983
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4146..4250
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4251..4319,4320..5111)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4469..4488)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5282..5870
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5771..5790
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6024..6041
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6158..6179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6194..6199,6200..6217,6218..6224)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6232..6248
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6237..6259
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6256..6272
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6256..6272
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6291..6311
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6293..6311
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        6412..6791
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6792..6995
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6946..6966
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7010..7190
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7237..7362
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7855..8088
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8273..8317
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8614..8731
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    9334..9358
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             9379..9405
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 tgcacagatt gtaattgacc atttaaggtc actgtgcagt ataacagatc ccgccaatga
       61 gaagctcttg actgtctttt gtggcatgct gatgaactat agcaatgaga atgattcgct
      121 tcaagctcag cttatcaata tgggtgttat tcctacctta gtgaaattac tgggcatcca
      181 ctgccaaaat gcagctctta cagaaatgtg tcttgttgca tttggtaatt tagcagaact
      241 tgagtcaagt aaagaacagt ttgccagtac aaacattgct gaagagctag taaaactctt
      301 caagaaacaa atagaacatg ataagagaga aatgattttt gaagttcttg ctccattggc
      361 agaaaatgat gctattaaac tacagctggt tgaagcaggc ctagtagagt gtctactaga
      421 gattgttcag caaaaagtgg atagtgacaa agaagatgat attactgagc tcaaaactgg
      481 ttcagatctc atggttttat tacttcttgg agatgaatcc atgcagaagt tatttgaagg
      541 aggaaaaggt agtgtatttc aaagggtact ctcttggatc ccatcaaata accaccagct
      601 acagcttgct ggagcattgg caattgcaaa ttttgccaga aatgatgcaa attgtattca
      661 tatggtagac aatgggattg tagaaaaact tatggattta ctggacagac atgtagaaga
      721 tggaaatgta acagtacagc atgcagcact aagtgccctc agaaacctgg ccattccagt
      781 tataaataaa gcaaagatgt tatcagctgg ggtcacagag gcagttttga aatttcttaa
      841 atctgaaatg cctcctgttc agttcaaact tctgggaaca ttaagaatgt taatagatgc
      901 acaagcagaa gctgctgaac aattgggaaa gaatgttaag ttagtggagc gtttggtgga
      961 atggtgtgaa gccaaagatc atgctggtgt gatgggggag tcaaacagac tgctgtctgc
     1021 ccttatacga cacagtaaat caaaagatgt aattaaaacc attgtgcaga gtggtggcat
     1081 caagcatcta gttaccatgg caactagtga acatgtaata atgcagaatg aagctcttgt
     1141 tgctttggca ttaatagcag ctttagaatt gggcactgct gagaaagatc tagaaagtgc
     1201 taaacttgta cagattttac atagactgct agcagatgag agaagtgctc ctgaaatcaa
     1261 atataattcc atggtcctga tatgtgctct tatgggatct gaatgtctac acaaggaagt
     1321 acaggatttg gcttttctag atgtcgtatc caaacttcgc agtcatgaga acaaaagtgt
     1381 tgcccagcag gcctctctca cagagcagag acttactgtg gaaagctagc tcgagtgcgg
     1441 ccgcaaccca gctttcttgt acaaagtggt gatatcggta ccgagctcga attctaccgg
     1501 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     1561 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     1621 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     1681 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     1741 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     1801 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     1861 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     1921 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     1981 tcatctccgg gcctttcgac catctagatc caccgcgggc caccatggtg agcaagggcg
     2041 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     2101 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2161 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     2221 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     2281 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2341 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     2401 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     2461 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     2521 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     2581 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     2641 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     2701 actccaccgg cggcatggac gagctgtaca agtagagcgg ccaactcgag ggatcccccg
     2761 gggtcgactg atcaaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg
     2821 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     2881 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     2941 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3001 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3061 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
     3121 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3181 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3241 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3301 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3361 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     3421 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     3481 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     3541 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     3601 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     3661 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     3721 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     3781 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     3841 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     3901 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     3961 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4021 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4081 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4141 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4201 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4261 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4321 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4381 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4441 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4501 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4561 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4621 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4681 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4741 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4801 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4861 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4921 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4981 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5041 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5101 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5161 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5221 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5281 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5341 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5401 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5461 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5521 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5581 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5641 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5701 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5761 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5821 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5881 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5941 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6001 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6061 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6121 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6181 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6241 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6301 ctcactaaag ggaacaaaag ctggagctgc aagcttggcc attgcatacg ttgtatccat
     6361 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
     6421 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     6481 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     6541 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     6601 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     6661 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     6721 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     6781 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     6841 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     6901 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     6961 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgg ggtctctctg
     7021 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7081 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7141 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7201 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7261 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7321 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7381 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7441 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7501 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7561 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7621 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7681 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7741 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7801 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7861 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     7921 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     7981 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8041 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8101 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8161 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8221 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8281 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8341 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8401 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8461 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     8521 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgata
     8581 agctaattca caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     8641 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     8701 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     8761 agtttgggaa ttagcttgat cgattagtcc aatttgttaa agacaggata tcagtggtcc
     8821 aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac gagccataga
     8881 tagaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta
     8941 ggtttggcaa gctaggatca aggttaggaa cagagagaca gcagaatatg ggccaaacag
     9001 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagttg gaacagcaga
     9061 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     9121 agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc
     9181 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
     9241 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
     9301 cactcggcgc gatctagatc tcgaatcgaa ttcacaagtt tgtacaaaaa agcaggcttg
     9361 aaggaattcg gtaccatgta tccgtatgat gtgccggatt atgcgtttaa caaatcattt
     9421 ggaacaccct ttgggggtgg cacaggtggc tttggcacaa cttcaacatt tggacagaat
     9481 actggctttg gcactactag tggaggggca tttggaacat ctgcatttgg ttctagcaac
     9541 aatactggag gcctctttgg aaattcacag actaaaccag gaggattgtt tggaaccagt
     9601 tcatttagcc agccagctac ctccacaagc actggctttg ggtttggtac gtcaacagga
     9661 acagcaaata ccttgtttgg aactgcaagc acagggacca gtctcttctc atcccaaaac
     9721 aatgcctttg cacaaaataa accaactggc tttggcaatt ttggaaccag tactagcagt
     9781 ggaggactct ttggaaccac aaataccacc tctaatcctt ttggcagcac atctggctcc
     9841 ctctttgggc caagtagttt tacagctgct cctactggga ctactattaa atttaaccct
     9901 ccaactggta cagatactat ggtcaaagct ggagttagca ctaacataag taccaagcac
     9961 cagtgtatta ctgctatgaa agaatatgaa agcaagtcac tagaggaact tcgtttagag
    10021 gattatcagg ctaacaggaa gggcccacag aaccaggtgg gagcaggtac cacaactggc
    10081 ttgtttgggt cttctccagc cacttccagc gcaacaggac tcttcagctc ctccaccact
    10141 aattcaggct ttgcatatgg tcagaacaaa actgcctttg gaactagtac aactggattt
    10201 ggaacaaatc caggtggtct ctttggccaa cagaatcagc agactaccag cctcttcagc
    10261 aaaccatttg gccaggctac aaccacccag aacactggct tttcctttgg taataccagc
    10321 accataggac agccaagcac caacaccatg ggattatttg gagtaaccca agcctcacag
    10381 cctggaggtc tttttgggac agctacaaac accagcactg ggacagcatt tggaacagga
    10441 acaggtctct ttgggcagac caatactgga tttggtgctg ttggttcgac cctgtttggc
    10501 aataacaagc ttactacatt tggaagcagc acaaccagtg caccttcatt tggtacaacc
    10561 agtggcgggc tctttggttt tggcacaaat accagtggga atagtatttt tggaagtaaa
    10621 ccagcacctg ggactcttgg aactgggctt ggtgcaggat ttggaacagc tcttggtgct
    10681 ggacaggcat ctttgtttgg gaacaaccaa cctaagattg gagggcctct tggtacagga
    10741 gcctttgggg cccctggatt taatactacg acagccactt tgggctttgg agccccccag
    10801 gccccagtag ataatctcag tgataccttg aagaagctga agataacagc tgttgacaag
    10861 actgaggata gtttagaagg atgcttggat tgtctgcttc aagccctggc tcaaaataat
    10921 acggaaacaa gtgaaaaaat ccaagcaagt ggaatacttc agctgtttgc aagtctgttg
    10981 actccacagt cttcctgcaa agccaaagta gctaacatca tagcagaagt agccaaaaat
    11041 gagtttatgc gaattccatg tgtggatgct ggattgattt caccactggt gcagctgcta
    11101 aatagcaaag accaggaagt gctgcttcaa acgggcaggg ctctaggaaa catatgttac
    11161 gatagccatg agggcagaag tgcagttgac caagcaggtg g
//
