LOCUS       sequence-475618-       10845 bp    DNA     circular SYN 10-JUN-2025
DEFINITION  Express NUP98::JADE2 in bicistronic retroviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10845)
  TITLE     Mullighan lab plasmids 2025
REFERENCE   2  (bases 1 to 10845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CL20_MSCV-NUP98::JADE2-ires-GFP
FEATURES             Location/Qualifiers
     source          1..10845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(267..286)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(414..436)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(630..649)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     749..771
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(809..827)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        895..999
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1000..1068,1069..1860)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1218..1237)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2031..2619
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2520..2539
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2773..2790
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2907..2928
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2943..2948,2949..2966,2967..2973)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2981..2997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     LTR             3159..3673
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     CDS             complement(join(3775..4488,4489..4491,4492..4494))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3820..3841)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4169..4188
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4428..4449
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    4506..5058
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4508..5075
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4668..4687)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     4897..4914
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     protein_bind    5088..5112
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9457..9481)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(9508..9924)
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     complement(9524..9540)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(9556..9578)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    9991..10332
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     LTR             join(10396..10845,1..67)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tttccatgcc ttgcaaaatg gcgttactta agctagcttg ccaaacctac aggtggggtc
       61 tttcattccc ccctttttct ggagactaaa taaaatcttt tattttatct atggctcgta
      121 ctctataggc ttcagctggt gatattgttg agtcaaaact agagcctgga ccactgatat
      181 cctgtcttta acaaattgga ctaatcgcct ctacgccgga cgcatcgtgg ccggcatcac
      241 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      301 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      361 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgc cgtcgtttta
      421 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
      481 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
      541 cgcagcctga atggcgaatg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
      601 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
      661 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
      721 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
      781 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      841 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      901 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      961 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     1021 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     1081 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     1141 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     1201 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     1261 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     1321 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     1381 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     1441 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     1501 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     1561 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     1621 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     1681 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     1741 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     1801 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     1861 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     1921 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     1981 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2041 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2101 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2161 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     2221 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2281 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2341 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     2401 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     2461 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     2521 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     2581 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     2641 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     2701 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     2761 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     2821 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     2881 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     2941 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     3001 cacaattctc cccagctctt tctgcgctgt ccataatgaa ctatttcagg agtattggag
     3061 tatgtggatt caaaacagtg gctaattgag tcaggattac ttaatattcg actcctaccc
     3121 tcagaatgtt gttctccaac ttcaagaaac tgttacccat gaaagacccc cgctgacggg
     3181 tagtcaatca ctcagaggag accctcccaa ggaacagcga gaccacaagt cggatgcaac
     3241 tgcaagaggg tttattggat acacgggtac ccgggcgacg cagtctatcg gaggactggc
     3301 gcgccgagtg aggggttgtg ggctctttta ttgagctcgg ggagcagaag cgcgcgaaca
     3361 gaagcgagaa gcgaactgat tggttagttc aaataaggca cagggtcatt tcaggtcctt
     3421 ggggcaccct ggaaacatct gatggttctc tagaaactgc tgagggcggg accgcatctg
     3481 gggaccatct gttcttggcc ctgagccggg gcaggaactg cttaccacag atatcctgtt
     3541 tggcccatat tctgctgtct ctctgttcct aaccttgatc tgaacttctc tattctcagt
     3601 tatgtatttt ccatgccttg caaaatggcg ttacttaagc tagcttgcca aacctacagg
     3661 tggggtcttt cattcccccc tttttctgga gactaaataa aatcttttat tttatcgata
     3721 agcttggctg caggtcgacg gtatcgataa gcttgataat tcttaattaa ttcattactt
     3781 gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat
     3841 gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt
     3901 gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag
     3961 ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcgccttga tgccgttctt
     4021 ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc
     4081 caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt
     4141 gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat
     4201 ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat
     4261 gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg
     4321 ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt
     4381 ggcatcgccc tcgccctcgc cagacacgct gaacttgtgg ccgtttacgt cgccgtccag
     4441 ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggttgt
     4501 ggccatatta tcatcgtgtt tttcaaagga aaaccacgtc cccgtggttc ggggggccta
     4561 gacgtttttt taacctcgac taaacacatg taaagcatgt gcaccgaggc cccagatcag
     4621 atcccataca atggggtacc ttctgggcat ccttcagccc cttgttgaat acgcttgagg
     4681 agagccattt gactctttcc acaactatcc aactcacaac gtggcactgg ggttgtgccg
     4741 cctttgcagg tgtatcttat acacgtggct tttggccgca gaggcacctg tcgccaggtg
     4801 gggggttccg ctgcctgcaa agggtcgcta cagacgttgt ttgtcttcaa gaagcttcca
     4861 gaggaactgc ttccttcacg acattcaaca gaccttgcat tcctttggcg agaggggaaa
     4921 gacccctagg aatgctcgtc aagaagacag ggccaggttt ccgggccctc acattgccaa
     4981 aagacggcaa tatggtggaa aataacatat agacaaacgc acaccggcct tattccaagc
     5041 ggcttcggcc agtaacgtta gggggggggg gcggaattcg ttatcgaacc actttgtaca
     5101 agaaagctgg gttgcggccg cactcgagct aggaggccag tacgcccatg cggaccacct
     5161 cctctgcccc tgcctcccgg ggaccccgct gcaccccacc gtcctcggcc tctacatctg
     5221 agtcgctcat ctccccatca gagaagtagc catcagtctc agtgtcaaaa tgcaggctga
     5281 ccttgggcct ctcagccaca gctgaaggtg gtcccatccc agcatcaggc ctggcacccg
     5341 gcaatctccg ggttaccttg ctctcgcggg gtggccggag ccggcccaaa ggcttagggc
     5401 ttgctgcagg gccaggcact tggacatctg agtcagcagc tactgaggct gcctcgtccg
     5461 gggtctcagg ggccagtggc ggggggcttt caggggtggc taggatggga cagtccccgg
     5521 ctgcagggct ggacggcaag tgagaagatg tccgacgtgg tggcttcctg gtaggtggcc
     5581 cttgaccccc cttgccactg cctgcatcct ggccccaggt cttcttgggg gctttgtctg
     5641 gagtcgtccg tgaaccaggc ccgtcctgcg gtggtggtgg tggtggtttc ttcttggcag
     5701 gcaggcgggt gcggcctcgg gccttcctag cagggtcacc aggtcgcagc gaggggtccc
     5761 gcatgaagct gagcagtgtc tcctcgtcct gcagcagttc ctctgacagc agccctggag
     5821 cagggtcctc gcggtgccca ttgttcagtg gccactcgct catggccatg ttctctgctg
     5881 tgatctgcac cgactgtgcc agccagctgt tgaagaaggt gccatcgatg gggaatgagg
     5941 tggacaggcc tcgacacaga tcctgttcaa taagtttcat ctgcaggtgg aatatctgct
     6001 cctggagttt gcagatggcg tgtttcgttc tctcgcgcct tgtcaccatg tagcacagat
     6061 ttctaaccct ctctaggtcc tgccgcagat gggtgaagag cttcaggcgg cggtagagga
     6121 cgtcctgctc ctgctgggcc aggttgtcca cctcgtcggt cttgggggtc agcagcggct
     6181 ggttggcatt ggctttcctc ttcagcttcc agtactggta gatgaagtcg accagtgcct
     6241 cagccaggtc cagccgctca gccacctcag ccggctccac cagctcgtag aagtcctcct
     6301 ctagctgctg cagccgctgc ttgcgcaggg tcaccttttc caggtcctcg ccagcctggc
     6361 tgggttccgt gggctcagat gtgggctcat tacgtgggcc cccgtcactg tgctcctggc
     6421 agaatgactt gaacttgacc tcatcgttgt ctgctaatat agtccgcatt tccaggccgt
     6481 ggtcaaaggc gcatgtgaca tggaacgctg tgacgcagga aggcatggaa cactggatgc
     6541 aggtgcctgt gcattccttg cagaggctgc aggacagagc ccagcggctg gctgggatat
     6601 gcgagatctt ggtgatgggc tccatcttct ctgggcagcc gatgctgacc tcaggaatcc
     6661 atagggcaca gctgacatgc acccacttgg tcccacttct agtgggcttc aaggctcctc
     6721 ctcgcttggg gcagagcagg cactttggct ggacacccag ggcacacgtc cggcacagcc
     6781 agctgcccgt gggcaccttg aggatcccgt agcatgcctg atgcacacag acgttgcact
     6841 tgtcacagaa gaccatctcg ttgccatcct cgccctcagg agagcgacac acgtcgcaga
     6901 caacatcctc gtcgtactcg atgcccagcc cctcctgcgt ctcaatggcc ctggccatat
     6961 tctggtggca cagggtctcc agctcctcca gcacacgctc taatgtcagc tcgtccagct
     7021 ccggcctctc catctcctta agctccgagt tgatgagctc cagccagtag gcatcaatct
     7081 cgtccaagtc atagcggctg ccccctggcc aatcaggctg ggagccctca ccaagcatgg
     7141 tgctgctcgg ggatgcctgg gcaggggggc cttccagtgg tgggaggatc ctcaccacgg
     7201 gctctgggat ggcctctgcc ccggcaggca cctgcacacc tttctcccat tcctgtcgcc
     7261 atgggtctgc caggatgtag tagtcatccg ggctgagctg gtatgagtcc gggatcttca
     7321 tggctgtgat caagtctgtc cggaaaacct cggagggctt cttttcgttc tgtcggggcc
     7381 agcccgactt ggtgctgctg ggcagtttgg agcatcttga tgcggatgta gatgtcgcat
     7441 gactgtcagt ggtgtcagag ttgtcactgc tgatggagta ttttcgcctc ttctcttcca
     7501 tcatggactc tggggcgccc ctagcccgcc ctgtccccag ggccgggccc gagcggcgat
     7561 tctcatcaac aggtttgctt aggaaactaa atctctctcc attttctgga tattcagatg
     7621 gtgaagctag attttctgaa tcacgattaa caggagaaaa gagattgcta ttattaaggt
     7681 tcttcaaaac caacttctta atgctcttct tgggcatgaa tgctccattg gctagggatg
     7741 gttcatcgtc atccagccca tcaaagagat gtgacttggc tgtgcctgtt gtttgtaaag
     7801 cctttggccg gactctagtg gcagggcggg gtgtcagttt ataatgagta ggtgtagtaa
     7861 gagccttctg ggctgctgga tttgttggtt tcaatctctc ttccttcttc ttagggtctg
     7921 acatcggatt ccggaagaga ggagagtctc caaaaggtga gtatgttaga ctattgatgt
     7981 gctgctggag aacagcctgc tgggcagcag aagcatttgg atctgtcaaa gctactgggg
     8041 cctggggggc tccaaagccc aaagtggctg tcgtagtatt aaatccaggg gccccaaagg
     8101 ctcctgtacc aagaggccct ccaatcttag gttggttgtt cccaaacaaa gatgcctgtc
     8161 cagcaccaag agctgttcca aatcctgcac caagcccagt tccaagagtc ccaggtgctg
     8221 gtttacttcc aaaaatacta ttcccactgg tatttgtgcc aaaaccaaag agcccgccac
     8281 tggttgtacc aaatgaaggt gcactggttg tgctgcttcc aaatgtagta agcttgttat
     8341 tgccaaacag ggtcgaacca acagcaccaa atccagtatt ggtctgccca aagagacctg
     8401 ttcctgttcc aaatgctgtc ccagtgctgg tgtttgtagc tgtcccaaaa agacctccag
     8461 gctgtgaggc ttgggttact ccaaataatc ccatggtgtt ggtgcttggc tgtcctatgg
     8521 tgctggtatt accaaaggaa aagccagtgt tctgggtggt tgtagcctgg ccaaatggtt
     8581 tgctgaagag gctggtagtc tgctgattct gttggccaaa gagaccacct ggatttgttc
     8641 caaatccagt tgtactagtt ccaaaggcag ttttgttctg accatatgca aagcctgaat
     8701 tagtggtgga ggagctgaag agtcctgttg cgctggaagt ggctggagaa gacccaaaca
     8761 agccagttgt ggtacctgct cccacctggt tctgtgggcc cttcctgtta gcctgataat
     8821 cctctaaacg aagttcctct agtgacttgc tttcatattc tttcatagca gtaatacact
     8881 ggtgcttggt acttatgtta gtgctaactc cagctttgac catagtatct gtaccagttg
     8941 gagggttaaa tttaatagta gtcccagtag gagcagctgt aaaactactt ggcccaaaga
     9001 gggagccaga tgtgctgcca aaaggattag aggtggtatt tgtggttcca aagagtcctc
     9061 cactgctagt actggttcca aaattgccaa agccagttgg tttattttgt gcaaaggcat
     9121 tgttttggga tgagaagaga ctggtccctg tgcttgcagt tccaaacaag gtatttgctg
     9181 ttcctgttga cgtaccaaac ccaaagccag tgcttgtgga ggtagctggc tggctaaatg
     9241 aactggttcc aaacaatcct cctggtttag tctgtgaatt tccaaagagg cctccagtat
     9301 tgttgctaga accaaatgca gatgttccaa atgcccctcc actagtagtg ccaaagccag
     9361 tattctgtcc aaatgttgaa gttgtgccaa agccacctgt gccaccccca aagggtgttc
     9421 caaatgattt gttaaacatg gttcgaactc cttccaagcc tgcttttttg tacaaacttg
     9481 tttgataacc tcgagagatc taattccggc gcctagagaa ggagtgaggg ctggataaag
     9541 ggaggatcga ggcggggtcg aacgaggagg ttcaaggggg agagacgggg cggatggagg
     9601 aagaggaggc ggaggcttag ggtgtacaaa gggcttgacc cagggagggg ggtcaaaagc
     9661 caaggcttcc caggtcacga tgtaggggac ctggtctggg tgtccatgcg ggccaggtga
     9721 aaagaccttg atcttaacct gggtgatgag gtctcggtta aaggtgccgt ctcgcggcca
     9781 tccgacgtta aaggttggcc attctgcaga gcagaaggta acccaacgtc tcttcttgac
     9841 atctaccgac tggttgtgag cgatccgctc gacatctttc cagtgaccta aggtcaaact
     9901 taagggagtg gtaacagtct ggccctaatt ttcagacaaa tacagaaaca cagtcagaca
     9961 gagacaacac agaacgatgc tgcagcgctg cagcagacaa gacgcgcggc ttcggttcca
    10021 aaccgaaagc aaaaattcag acggaggcgg gaactgtttt aggttctcgt ctcctaccag
    10081 aaccacatat cctgacgggg tcggattcca catcgactcc cttcctcagg tcgggccaca
    10141 aaaacggccc ccaaagtccc tgggacgtct cccagggttg cggccgggtg ttcagaactc
    10201 gtcagttcca ccacgggtcc gccagataca gagctagtta gctaactagt acagacgcag
    10261 gcgcaaacat tagatgccgg cacaaacacg cacaaagaca gagacagaca cgaaacgacc
    10321 gctggccagc ttacctcccg gcgggggggt cggtggtccc tgggcagggg tctccaaatc
    10381 tcggtggaac ctccaaatga aagacccccg aggtgggcag tcaatcaatc tgaggagacc
    10441 ctcccaagga tcagcgagtc cacgattcgg atgcaaacag caagaggctt tattgggaat
    10501 acgggtaccc gggcgacgca gtctatcgga ggactggcgc gccgagtgag gggttgtggg
    10561 ctcttttatt gagctcgggg agcagaagcg cgcgaacaga agcgagaagc gaactgattg
    10621 gttagttcaa ataaggcaca gggtcatttc aggtccttgg ggcaccctgg aaacatctga
    10681 tggttctcta gaaactgctg agggcgggac cgcatctggg gaccatctgt tcttggccct
    10741 gagccggggc aggaactgct taccacagat atcctgtttg gcccatattc tgctgtctct
    10801 ctgttcctaa ccttgatctg aacttctcta ttctcagtta tgtat
//
