LOCUS       sequence-475633-       13376 bp    DNA     circular SYN 11-JUN-2025
DEFINITION  Express HA-tagged NUP98 in dual promoter lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13376)
  TITLE     Mullighan lab plasmids 2025
REFERENCE   2  (bases 1 to 13376)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CCLMPC_HA-NUP98
FEATURES             Location/Qualifiers
     source          1..13376
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(105..124)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      918..1506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1407..1426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1660..1677
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1794..1815
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1830..1835,1836..1853,1854..1860)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1868..1884
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1873..1895
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1892..1908
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1892..1908
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1927..1947
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1929..1947
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2048..2427
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2428..2631
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2582..2602
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2646..2826
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2873..2998
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3491..3724
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3909..3953
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4250..4367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4970..4994
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5015..5041
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(10458..10482)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        10511..11010
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10546..10569)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10943..10962
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      11031..11040
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11037..11747
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(11182..11200)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     11450..11469
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             11869..12102
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    12174..12295
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12211..12230)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12265..12284
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      12335..12470
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(12490..12509)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12491..12509)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12519..12536)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12519..12535)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12528..12550)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12677..13132
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12764..12783)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12974..12995
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        13158..13262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13263..13331,13332..13376,1..747)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
       61 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      121 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      181 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      241 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      301 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      361 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      421 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      481 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      541 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      601 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
      661 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
      721 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
      781 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
      841 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
      901 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
      961 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1021 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     1081 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     1141 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     1201 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     1261 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     1321 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     1381 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     1441 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     1501 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     1561 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     1621 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     1681 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     1741 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
     1801 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
     1861 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc
     1921 aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttggccattg
     1981 catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg
     2041 ccatgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     2101 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     2161 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     2221 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     2281 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     2341 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     2401 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     2461 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     2521 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     2581 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
     2641 aaccggggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     2701 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     2761 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     2821 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     2881 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     2941 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     3001 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     3061 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     3121 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     3181 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     3241 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     3301 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     3361 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     3421 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     3481 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     3541 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     3601 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     3661 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     3721 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     3781 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     3841 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     3901 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     3961 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     4021 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     4081 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     4141 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     4201 ctcgacggta tcgataagct aattcacaaa tggcagtatt catccacaat tttaaaagaa
     4261 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     4321 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     4381 gggacagcag agatccagtt tgggaattag cttgatcgat tagtccaatt tgttaaagac
     4441 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
     4501 gagtacgagc catagataga ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
     4561 aaagacccca cctgtaggtt tggcaagcta ggatcaaggt taggaacaga gagacagcag
     4621 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     4681 cagttggaac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     4741 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga
     4801 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     4861 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     4921 agagcccaca acccctcact cggcgcgatc tagatctcga atcgaattca caagtttgta
     4981 caaaaaagca ggcttggaag gagttcgaac catgtaccca tacgatgttc cagattacgc
     5041 ttttaacaaa tcatttggaa caccctttgg gggtggcaca ggtggctttg gcacaacttc
     5101 aacatttgga cagaatactg gctttggcac tactagtgga ggggcatttg gaacatctgc
     5161 atttggttct agcaacaata ctggaggcct ctttggaaat tcacagacta aaccaggagg
     5221 attgtttgga accagttcat ttagccagcc agctacctcc acaagcactg gctttgggtt
     5281 tggtacgtca acaggaacag caaatacctt gtttggaact gcaagcacag ggaccagtct
     5341 cttctcatcc caaaacaatg cctttgcaca aaataaacca actggctttg gcaattttgg
     5401 aaccagtact agcagtggag gactctttgg aaccacaaat accacctcta atccttttgg
     5461 cagcacatct ggctccctct ttgggccaag tagttttaca gctgctccta ctgggactac
     5521 tattaaattt aaccctccaa ctggtacaga tactatggtc aaagctggag ttagcactaa
     5581 cataagtacc aagcaccagt gtattactgc tatgaaagaa tatgaaagca agtcactaga
     5641 ggaacttcgt ttagaggatt atcaggctaa caggaagggc ccacagaacc aggtgggagc
     5701 aggtaccaca actggcttgt ttgggtcttc tccagccact tccagcgcaa caggactctt
     5761 cagctcctcc accactaatt caggctttgc atatggtcag aacaaaactg cctttggaac
     5821 tagtacaact ggatttggaa caaatccagg tggtctcttt ggccaacaga atcagcagac
     5881 taccagcctc ttcagcaaac catttggcca ggctacaacc acccagaaca ctggcttttc
     5941 ctttggtaat accagcacca taggacagcc aagcaccaac accatgggat tatttggagt
     6001 aacccaagcc tcacagcctg gaggtctttt tgggacagct acaaacacca gcactgggac
     6061 agcatttgga acaggaacag gtctctttgg gcagaccaat actggatttg gtgctgttgg
     6121 ttcgaccctg tttggcaata acaagcttac tacatttgga agcagcacaa ccagtgcacc
     6181 ttcatttggt acaaccagtg gcgggctctt tggttttggc acaaatacca gtgggaatag
     6241 tatttttgga agtaaaccag cacctgggac tcttggaact gggcttggtg caggatttgg
     6301 aacagctctt ggtgctggac aggcatcttt gtttgggaac aaccaaccta agattggagg
     6361 gcctcttggt acaggagcct ttggggcccc tggatttaat actacgacag ccactttggg
     6421 ctttggagcc ccccaggccc cagtagcttt gacagatcca aatgcttctg ctgcccagca
     6481 ggctgttctc cagcagcaca tcaatagtct aacatactca ccttttggag actctcctct
     6541 cttccggaat ccgatgtcag accctaagaa gaaggaagag agattgaaac caacaaatcc
     6601 agcagcccag aaggctctta ctacacctac tcattataaa ctgacacccc gccctgccac
     6661 tagagtccgg ccaaaggctt tacaaacaac aggcacagcc aagtcacatc tctttgatgg
     6721 gctggatgac gatgaaccat ccctagccaa tggagcattc atgcccaaga agagcattaa
     6781 gaagttggtt ttgaagaacc ttaataatag caatctcttt tctcctgtta atcgtgattc
     6841 agaaaatcta gcttcaccat ctgaatatcc agaaaatgga gagagattta gtttcctaag
     6901 caaacctgtt gatgagaatc accagcagga tggagatgaa gattcccttg tttcacattt
     6961 ttatactaac cctattgcca aacctattcc tcaaacccca gaaagtgctg gaaataaaca
     7021 cagcaacagc aacagtgtgg atgataccat tgttgcatta aacatgcgtg ctgctttgcg
     7081 aaatgggctg gaaggaagca gtgaagaaac gtcttttcat gatgagtcac ttcaggatga
     7141 ccgagaagaa atagaaaata attcttacca tatgcaccca gcaggtatta ttctcactaa
     7201 ggttggttac tatactattc catctatgga tgaccttgct aaaattacca atgaaaaagg
     7261 agagtgcatt gtctctgatt tcactattgg tcggaaaggt tatggttcaa tctattttga
     7321 aggagatgtg aatttgacaa atctaaattt ggatgatatt gtgcatatcc ggaggaaaga
     7381 agtagttgtc tacttagatg ataaccaaaa accacctgtg ggtgaagggc taaataggaa
     7441 ggctgaagtt acattggatg gagtttggcc aacagataaa acatctcgtt gtttaataaa
     7501 gagcccagat cgccttgctg atatcaacta tgaaggaaga ttggaagcag tttcaaggaa
     7561 acagggagct caattcaaag aataccggcc tgaaactggt tcttgggtgt ttaaggtctc
     7621 ccatttttct aagtatggcc ttcaggattc tgatgaagag gaggaggagc atccgtctaa
     7681 aactagtaca aagaagttga agactgctcc tttgcctcct gcaagccaga ctacgccctt
     7741 gcagatggct cttaatggca aacctgcacc tccacctcag agccagagcc cagaggtgga
     7801 gcagttaggg agggttgtgg aactggacag tgacatggta gatatcaccc aggagccagt
     7861 tttggatacc atgttagaag agagcatgcc tgaggatcag gaacctgtgt ctgcctcaac
     7921 acatattgca tcttcactgg gaattaatcc acatgtctta cagatcatga aagcatcatt
     7981 gcttactgat gaagaagatg tagatatggc actggatcaa cgcttcagtc gcctgccttc
     8041 caaagcagat acttctcaag aaatctgttc tcccagactc cccatttcag catcccactc
     8101 gtcgaaaact cgttcactag ttggtgggtt actacaatca aaatttacaa gtggagcttt
     8161 tctttcacca agtgtctctg tgcaagaatg tcgtactccc agagcagcat ctttaatgaa
     8221 tatcccatcc acatcctctt ggtctgtccc tccacccctg acttctgtgt tcacaatgcc
     8281 cagcccagcc cctgaggttc cgttgaaaac agtgggtaca cgtaggcaac taggcctagt
     8341 ccctcgtgaa aagtctgtca cctatggcaa gggaaaactc ttgatggaca tggccctatt
     8401 catgggacgc tcatttcgtg ttggttgggg ccccaactgg actcttgcta atagtggaga
     8461 acagctgaat ggctctcatg aactagaaaa tcatcagatt gccgattcca tggagtttgg
     8521 attcctgccc aatccagtag ctgttaaacc cctaactgag tccccattca aagttcactt
     8581 agaaaaactc agtttgagac aaagaaagcc agatgaagac atgaaattat atcagacacc
     8641 tctggagctc aaattaaaac acagcactgt ccatgtggat gaactgtgtc ctctcattgt
     8701 ccccaatctg ggagttgctg tcattcatga ctatgcagat tgggttaaag aagcatcagg
     8761 agacttacca gaagcacaaa ttgtgaagca ctggagcctg acatggacac tatgtgaagc
     8821 cctatggggc cacctgaagg agcttgacag ccagctaaat gaaccccgtg aatacattca
     8881 aattctggag cgaagaagag ctttctcccg ctggctatcc tgtactgcca cacctcagat
     8941 tgaagaggaa gtctccttaa cccaaaaaaa cagccctgtg gaggctgtat tcagctacct
     9001 cacaggcaaa aggatcagtg aggcctgctc tctggcccag cagtcagggg atcatcgtct
     9061 tgctcttctt ttatctcagt ttgtgggtag ccagtcagtc cgggagctgc tcaccatgca
     9121 gttggtggac tggcatcagc tccaagcaga ctccttcatc caggatgaga gactgcgcat
     9181 ctttgctctg ttggctggaa aaccggtgtg gcagctctca gaaaagaagc aaataaacgt
     9241 gtgctcccag ttggattgga aacgctccct ggctatccat ctttggtatt tgcttccacc
     9301 aacagcctcc atttctaggg cgctcagcat gtatgaagaa gcatttcaga atacctctga
     9361 cagtgacaga tatgcctgct ccccacttcc ttcgtatctg gagggttctg gctgtgtgat
     9421 agcggaggag caaaactcac agacaccact tcgagatgtc tgctttcacc ttctaaaact
     9481 ctacagtgac agacattatg atctcaacca gctgctggag cctcgaagca taacagcaga
     9541 tcctttggac taccgcctaa gctggcactt gtgggaagtg ctgagggctc ttaactacac
     9601 ccatctctca gcgcagtgtg aaggtgtgct acaggccagt tacgctggcc agcttgaaag
     9661 tgaggggctc tgggagtggg ccatctttgt cctcctgcac attgacaact caggcatacg
     9721 tgagaaggct gttcgagagc tgcttacccg gcactgccag ctgttggaga cccctgaatc
     9781 ttgggctaaa gagactttcc ttacccagaa gctccgtgta cctgccaaat ggatccacga
     9841 ggccaaagct gtgcgagcac acatggaatc tgacaagcac ttagaggccc tttgcttatt
     9901 taaggctgag cactggaacc gctgccacaa gctcatcatc cgacacttag cttctgatgc
     9961 catcattaat gagaactatg actacctgaa ggggttcttg gaagacctgg cacctccaga
    10021 gcgcagcagc ctaattcagg attgggaaac atctgggctt gtttacctgg actatattag
    10081 agtcattgaa atgctccgcc atatacagca ggtggattgc tcaggtaatg acctggagca
    10141 gttacacatc aaagtgactt cactgtgcag tcggatagag cagattcagt gttacagtgc
    10201 taaagatcgc ctggctcagt cagacatggc caaacgtgta gccaacctgc tgcgcgtggt
    10261 gctgagtctg catcatcctc ctgatagaac ctccgactca acaccagacc ctcagcgagt
    10321 ccctttgcgc ctcttggctc cccacattgg ccggcttccc atgcctgagg actatgccat
    10381 ggacgaactg cgcagcctta cccagtccta tctgcgagaa ctggctgttg ggagcctgta
    10441 gctcgagtgc ggccgcaacc cagctttctt gtacaaagtg gtgatatcgg taccgagctc
    10501 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
    10561 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
    10621 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
    10681 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
    10741 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
    10801 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
    10861 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
    10921 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
    10981 ttctcctctt cctcatctcc gggcctttcg accatctaga tccaccgcgg gccaccatgg
    11041 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
    11101 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
    11161 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
    11221 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
    11281 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
    11341 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
    11401 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
    11461 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
    11521 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
    11581 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
    11641 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
    11701 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtagagc ggccaactcg
    11761 agggatcccc cggggtcgac tgatcaaatt cgagctcggt acctttaaga ccaatgactt
    11821 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
    11881 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
    11941 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
    12001 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
    12061 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
    12121 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
    12181 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12241 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    12301 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
    12361 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
    12421 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
    12481 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    12541 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    12601 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    12661 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
    12721 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
    12781 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
    12841 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
    12901 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
    12961 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
    13021 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
    13081 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
    13141 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
    13201 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
    13261 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
    13321 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttg
//
