LOCUS       sequence-475875-       11349 bp    DNA     circular SYN 12-JUN-2025
DEFINITION  Express UTR1 membrane protein cargo, peroxisomal Ynd1p truncation, 
            and inactive Pex22 peroxisomal chauffeur as negative control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11349)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1428
FEATURES             Location/Qualifiers
     source          1..11349
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3346..3759
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     terminator      5319..5506
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5374..5398)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     promoter        5850..6263
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     CDS             6397..7104
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     CDS             7622..8425
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(9067..9882)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9067..9882)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9790..9809
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9874..9893
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10107..10695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10596..10615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgttgat aggtcaagat caatgtaaac aattactttg
       61 ttatgtagag tttttttagc tacctatatt ccaccataac atcaatcatg cggttgctgg
      121 tgtatttacc aataatgttt aatgtatata tatatatata tatatggggc cgtatactta
      181 catatagtag atgtcaagcg taggcgcttc ccctgccggc tgtgagggcg ccataaccaa
      241 ggtatctata gaccgccaat cagcaaacta cctccgtaca ttcatgttgc acccacacat
      301 ttatacaccc agaccgcgac aaattaccca taaggttgtt tgtgacggcg tcgtacaaga
      361 gaacgtggga actttttagg ctcaccaaaa aagaaagaaa aaatacgagt tgctgacaga
      421 agcctcaaga aaaaaaaaat tcttcttcga ctatgctgga ggcagagatg atcgagccgg
      481 tagttaacta tatatagcta aattggttcc atcaccttct tttctggtgt cgctccttct
      541 agtgctattt ctggcttttc ctattttttt ttttccattt ttctttctct ctttctaata
      601 tataaattct cttgcatttt ctatttttct ctctatctat tctacttgtt tattcccttc
      661 aaggtttttt tttaaggagt acttgttttt agaatatacg gtcaacgaac tataattaac
      721 taaacagatc tatggaggtt cacggttcag gattcagaag aatattatta ttggcattat
      781 gtatttcagg tatttggtca gcatatatct accaaggtgt tttacaagaa accttatcaa
      841 caaagagatt tggtcctgat gagaagagat ttgaacattt agcattcttg aatttggccc
      901 aatctgtcgt ttgtttgatt tggtcttata ttatgatcaa gttatggtca aacgctggta
      961 atggtggagc tccatggtgg acttattggt cagccggtat aaccaacacc ataggaccag
     1021 ctatgggtat tgaggcatta aaatatatat cttacccagc acaagtttta gcaaaatcat
     1081 ctaaaatgat accagttatg ttaatgggaa ccttggttta cggaataaga tatacatttc
     1141 cagaatacat gtgcactttc ttggttgcag gtggtgtctc tatatttgca ttgttaaaga
     1201 cctcttctaa gactatctct aagttggcac atcctaatgc ccctttggga tacgcattat
     1261 gctcattaaa tttggcattt gatggattta ctaatgcaac ccaggattct attgcatcta
     1321 ggtatcctaa aactgaggct tgggatatta tgttaggtat gaatttgtgg ggtactattt
     1381 ataatatgat ctacatgttc ggattacctc aaggtattgg atttaaagca attcagtttt
     1441 gcaagttaca ccctgaagcc gcttgggata tattgaagta ctgcatttgt ggagctgtag
     1501 gacaaaattt tatctttatg acaatatcta attttggttc attggcaaat actacaataa
     1561 ctacaaccag aaagttcgtt tcaattgttg tatcatctgt aatgtctggt aacccattat
     1621 ctttaaaaca gtggggttgt gtatctatgg tctttggtgg tttagcttac caaatctatt
     1681 taaaatggaa gaaattacaa agagttgaag gttctgtttc taaaggtgaa gaattattca
     1741 ctggtgttgt cccaattttg gttgaattag atggtgatgt taatggtcac aaattttctg
     1801 tctccggtga aggtgaaggt gatgctactt acggtaaatt gaccttaaaa tttatttgta
     1861 ctactggtaa attgccagtt ccatggccaa ccttagtcac tactttatct tggggtgttc
     1921 aatgttttgc aagataccca gatcatatga aacaacatga ctttttcaag tctgccatgc
     1981 cagaaggtta tgttcaagaa agaactattt ttttcaaaga tgacggtaac tacaagacca
     2041 gagctgaagt caagtttgaa ggtgatacct tagttaatag aatcgaatta aaaggtattg
     2101 attttaaaga agatggtaac attttaggtc acaaattgga atacaattat ttctctgaca
     2161 atgtttacat cactgctgac aaacaaaaga atggtatcaa agctaacttc aaaattagac
     2221 acaacattga agatggtggt gttcaattag ctgaccatta tcaacaaaat actccaattg
     2281 gtgatggtcc agtcttgtta ccagacaacc attacttatc cactcaatct aagttatcca
     2341 aagatccaaa cgaaaagagg gaccacatgg tcttgttaga atttgttact gctgctggta
     2401 ttaccttggg tatggatgaa ttgtacaaag gatccgcaga gtatgtgcgg gccctgtttg
     2461 actttaatgg gaatgatgaa gaagaccttc cctttaagaa aggagacatc ctgagaatcc
     2521 gggataagcc tgaagagcag tggtggaatg cagaggacag cgaaggaaag agggggatga
     2581 ttcctgtccc ttacgtggag aagtatggat ctgggagcgg atctggaggc agcatggcgg
     2641 aatatgtacg agctcttttt gattttaatg gcaatgatga ggaggatctc ccttttaaaa
     2701 agggcgatat tctgcgcatt cgtgataagc cagaggaaca atggtggaac gccgaggata
     2761 gtgaggggaa acgcggcatg attccggtgc cgtatgtaga gaagtactaa tggcgccaat
     2821 tggtgcggca attgataata acgaaaatgt cttttaatga tctgggtata atgaggaatt
     2881 ttccgaacgt ttttacttta tatatatata tacatgtaac atatattcta tacgctatag
     2941 agaaaggaaa tttttcaatt aaaaaaaaaa tagagaaaga gtttcacttc ttgattatcg
     3001 ctaacactaa tggttgaagt actgctactt taattttatg ctgccaaacc agatgtcaac
     3061 acagctacaa cgccttgcca acagggagtt cttcagagac atggaggctc aaaacgaaat
     3121 tattgacagc ctagacatca atagtcatac aacagaaagc gaccacccaa ctttggctga
     3181 taatagcgta taaacaatgc atactttgta cgttcaaaat acaatgcagt agatatattt
     3241 atgcatatta catataatac atatcacata ggaagcaaca ggcgcgttgg acttttaatt
     3301 ttcgaggacc gcgaatcctt acatcacacc caatccccca caagtgatcc cccacacacc
     3361 atagcttcaa aatgtttcta ctcctttttt actcttccag attttctcgg actccgcgca
     3421 tcgccgtacc acttcaaaac acccaagcac agcatactaa atttcccctc tttcttcctc
     3481 tagggtgtcg ttaattaccc gtactaaagg tttggaaaag aaaaaagaca ccgcctcgtt
     3541 tctttttctt cgtcgaaaaa ggcaataaaa atttttatca cgtttctttt tcttgaaaat
     3601 tttttttttt gatttttttc tctttcgatg acctcccatt gatatttaag ttaataaacg
     3661 gtcatcaatt tctcaagttt cagtttcatt tttcttgttc tattacaact ttttttactt
     3721 cttgctcatt agaaagaaag catagcaatc taatctaagt tttaattaca aaagatctat
     3781 gctcatagaa aacactaatg atcggtttgg tatcgtcata gatgcagggt cttcgggttc
     3841 gagaatccat gtgtttaagt ggcaggacac ggaatcgctt cttcatgcaa caaaccagga
     3901 ctcacagtcc atattacaat cagtacctca tattcatcaa gaaaaagact ggacttttaa
     3961 gctgaatcca ggcttgtcga gttttgaaaa aaaacctcaa gatgcgtaca aatctcacat
     4021 caagccgtta ctagattttg ctaagaatat tatccctgaa tcacattggt cgagttgtcc
     4081 cgtttttatt caagcaaccg cgggcatgcg ccttttacct caagacatac aatcttccat
     4141 tttggatggt ttgtgtcagg ggctcaaaca ccctgcagaa tttttggttg aggattgctc
     4201 agcacaaatt caagtcattg atggtgaaac cgaaggttta tatggctggc ttggcttaaa
     4261 ctatctatat ggacacttta atgactataa tccagaagtc tctgaccatt ttacatttgg
     4321 gtttatggac atgggcggtg cctctactca gattgcgttt gcaccgcatg attcaggaga
     4381 aatagctaga catagagatg acatcgccac catcttcttg aggagtgtta acggagattt
     4441 gcagaaatgg gacgtttttg ttagtacgtg gttagggttt ggtgccaatc aggctagaag
     4501 aaggtacttg gctcagttga tcaataccct tccagaaaac acaaatgatt acgaaaatga
     4561 tgacttctct acaaggaact tgaatgatcc atgtatgccg cgaggaagca gcacggattt
     4621 tgaatttaaa gataccatat ttcacatcgc gggttctggg aattacgaac aatgtacaaa
     4681 atctatttat ccgttacttt taaagaacat gccttgtgat gacgagccgt gcttgtttaa
     4741 tggtgttcat gctcctcgaa tagactttgc caatgacaaa tttataggta cttctgagta
     4801 ctggtacact gccaacgacg tattcaaact tgggggcgaa tacaactttg acaaatttag
     4861 caaaagccta agggagtttt gcaattccaa ttggacgcaa atattagcga acagtgataa
     4921 aggcgtgtat aattctattc cggagaattt tttgaaagat gcatgcttta agggcaattg
     4981 ggtccttaat atacttcatg aagggtttga tatgcctcga atagatgtcg acgcagaaaa
     5041 tgtcaatgac aggcccttat ttcaaagtgt ggaaaaagtc gaagaacgag agttatcgtg
     5101 gacactaggc agaattttac tttatgcctc aggtagcata ttggcaggta atgatgactt
     5161 catggtgggt attgcgccca gtgaaagaag aactaagctc actggtaaga aattcatacc
     5221 tggcaagtta ctggagtctg atcaactacg caagcaaagt tccggatccg gttcattggg
     5281 aagaggtaga agatccaaat tgtaataact cgagtggcgc gaatttctta tgatttatga
     5341 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt
     5401 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt
     5461 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggcatg ccgagcaaat
     5521 gcctgcaaat cgctccccat ttcgctggat gcacacactg gcttaagatg acaacgcctt
     5581 gccaacaggg agttcttcag agacatggag gctcaaaacg aaattattga cagcctagac
     5641 atcaatagtc atacaacaga aagcgaccac ccaactttgg ctgataatag cgtataaaca
     5701 atgcatactt tgtacgttca aaatacaatg cagtagatat atttatgcat attacatata
     5761 atacatatca cataggaagc aacaggcgcg ttggactttt aattttcgag gaccgcgaat
     5821 ccttacatca cacccaatcc cccacaagtg atcccccaca caccatagct tcaaaatgtt
     5881 tctactcctt ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca
     5941 aaacacccaa gcacagcata ctaaatttcc cctctttctt cctctagggt gtcgttaatt
     6001 acccgtacta aaggtttgga aaagaaaaaa gacaccgcct cgtttctttt tcttcgtcga
     6061 aaaaggcaat aaaaattttt atcacgtttc tttttcttga aaattttttt ttttgatttt
     6121 tttctctttc gatgacctcc cattgatatt taagttaata aacggtcatc aatttctcaa
     6181 gtttcagttt catttttctt gttctattac aacttttttt acttcttgct cattagaaag
     6241 aaagcatagc aatctaatct aagttttaat tacaaaagat ctatgccacc accatcaaga
     6301 agtagaataa acaaaacaag aacattagga atagtgggta cagctatagc agtgttggtc
     6361 acgtcctact atatatatca aaaggtgaca ggttctgtgt ccaaaggaga ggagttaatc
     6421 aaggaaaaca tgagaatgaa agttgtcatg gagggctccg ttaatggtca ccaattcaag
     6481 tgtacagggg aaggtgaagg taatccttac atgggtacac aaactatgag aattaaagta
     6541 attgaaggcg gaccactacc atttgcattt gacattctgg caacgtcatt catgtacgga
     6601 tcacgaactt tcatcaagta ccctaaaggt ataccagact ttttcaagca atcttttcca
     6661 gagggtttta catgggaaag ggttacaaga tacgaagatg ggggtgtcgt cacagttatg
     6721 caagatactt cattagaaga tggctgcctt gtctatcatg tgcaagtaag aggggtgaat
     6781 tttccttcta acggacctgt gatgcagaaa aagaccaaag gttgggaacc aaatactgaa
     6841 atgatgtacc cagctgatgg aggtttgaga ggctacacac acatggcgct taaagttgat
     6901 ggtggaggtc atttgtcttg tagttttgtt accacttatc gttctaaaaa gactgttggc
     6961 aatatcaaaa tgccaggaat acatgctgta gaccacagac tagaaagact cgaagagagc
     7021 gataacgaaa tgttcgttgt acagagagag catgccgtag ccaaatttgc tggcttaggc
     7081 ggtggtatgg atgaattgta taagggatcc gggtcaggct ccggatctgg ccctcccgca
     7141 gctcttgcgc caaaaagacg aaggtaatgg cagtgctttt aactaagaat tattagtctt
     7201 ttctgcttat tttttcatca tagtttagaa cactttatat taacgaatag tttatgaatc
     7261 tatttaggtt taaaaattga tacagtttta taagttactt tttcaaagac tcgtgctgtc
     7321 tattgcataa tgcactggaa ggggaaaaaa aaggtgcaca cgcgtggctt tttcttgaat
     7381 ttgcagtttg aaaaatgctg agcagttaca gagatgttac gaaccactag tgcactgcag
     7441 tacacggttt ccttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag
     7501 gaaggagcac agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa
     7561 ttgcccagta ttcttaaccc aactgcacag aacaaaaacg tgcaggaaac gaagataaat
     7621 catgtcgaaa gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa
     7681 gctatttaat atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac
     7741 caccaaggaa ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac
     7801 acatgtggat atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt
     7861 atccgccaag tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac
     7921 agtcaaattg cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa
     7981 tgcacacggt gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt
     8041 aacaaaggaa cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc
     8101 tactggagaa tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat
     8161 cggctttatt gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat
     8221 gacacccggt gtgggtttag atgacaaggg agatgcattg ggtcaacagt atagaaccgt
     8281 ggatgatgtg gtttctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa
     8341 gggaagggat gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt
     8401 gagaagatgc ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa
     8461 ctcacaaatt agagcttcaa tttaattata tcagttatta cccgagtaga gcacttgaat
     8521 ccactgcccc gggaatctcg gtcgtaatga tttctataat gacgaaaaaa aaaaaattgg
     8581 aaagaaaaag cttcatggcc tttataaaaa ggaactatcc aatacctcgc cagaaccaag
     8641 taacagtatt ttacggggca caaatcaaga acaataagac aggactgtaa agatggacgc
     8701 attgaactcc aaagaacaac aagagttcca aaaagtagtg gaacaaaagc aaatgaagga
     8761 tttcatgcgt ttgtactcta atctggtaga aagatgtttc acagactgtg tcaatgactt
     8821 cacaacatca aagctaacca ataaggaaca aacatgcatc atgaagtgct cagaaaagtt
     8881 cttgaagcat agcgaacgtg tagggcagcg tttccaagaa caaaacgctg ccttgggaca
     8941 aggcttgggc cgataaggtg tactggcgta tatatatcta attatgtatc tctggtgtag
     9001 cccattttta gcatgtaaat ataaagaccg agcggccgcg tgttacaacc aattaaccaa
     9061 ttctgattag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     9121 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     9181 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     9241 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     9301 gacgactgaa tccggtgaga atggcaaaag cttatgcatt tctttccaga cttgttcaac
     9361 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     9421 tgattgcgcc tgagcgaggc gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     9481 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     9541 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     9601 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     9661 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     9721 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     9781 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     9841 tcgcggcctg gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     9901 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
     9961 acatcagaga ttttgagaca caacgtggct ttgttgaata aatcgaactt ttgctgagtt
    10021 gaaggatcag tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10081 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    10141 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    10201 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
    10261 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    10321 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    10381 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    10441 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    10501 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    10561 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    10621 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    10681 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    10741 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtgcggcc
    10801 gccaatgcta aattcgagtg aaacacagga agatcagaaa atcctcattt catccatatt
    10861 aacaataatt tcaaatgttt atttgcatta tttgaaacta ggcaagacaa gcaacgaaac
    10921 gtttttgaaa attttgagta ttttcaataa atttgtagag gactcagata ttgaaaaaaa
    10981 gctacagcaa ttaatacttg ataagaagag tattgagaag ggcaacggtt catcatctca
    11041 tggatctgca catgaacaaa caccagagtc aaacgacgtt gaaattgagg ctactgcgcc
    11101 aattgatgac aatacagacg atgataacaa accgaagtta tctgatgtag aaaaggatta
    11161 aagatgctaa gagatagtga tgatatttca taaataatgt aattctatat atgttaatta
    11221 ccttttttgc gaggcatatt tatggtgaag gataagtttt gaccatcaaa gaaggttaat
    11281 gtggctgtgg tttcagggtc cataaagccc acatggataa cattacccct gaattcgcat
    11341 ctagactga
//
