LOCUS       sequence-475882-       15236 bp    DNA     circular SYN 12-JUN-2025
DEFINITION  proUBI10::VIH2-FL::FLAG-Wild type ubiquitous expression in 
            Arabidopsis plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Raia P, Lee K, Bartsch SM, Rico-Resendiz F, Portugal-Calisto D, 
            Vadas O, Panse VG, Fiedler D, Hothorn M
  TITLE     A small signaling domain controls PPIP5K phosphatase activity in 
            phosphate homeostasis.
  JOURNAL   Nat Commun. 2025 Feb 19;16(1):1753. doi: 10.1038/s41467-025-56937-0.
  PUBMED    39966396
REFERENCE   2  (bases 1 to 15236)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-02-19"; volume: "16(1):1753. doi"; pages: " 
            10.1038/s41467-025-56937-0"
COMMENT     Sequence Label: proUBI10::VIH2-FL::FLAG-Wild type
FEATURES             Location/Qualifiers
     source          1..15236
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    775..915
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(841..860)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1001..1023
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      1259..1453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(1519..2592)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(3021..3650)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4951..4975
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9680..9691)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             10168..10233
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      10241..10493
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13621..13645
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             complement(14023..14814)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
      841 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
      901 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
      961 ctgctcccgg catccgctta cagacaagct gtgactgtct ccgggagctg catgtgtcag
     1021 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
     1081 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
     1141 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
     1201 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
     1261 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
     1321 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
     1381 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
     1441 cgtgctatcc acaggaaaga gtccttttcg acctttttcc cctgctaggg caatttgccc
     1501 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
     1561 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
     1621 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
     1681 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
     1741 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
     1801 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
     1861 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
     1921 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
     1981 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
     2041 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
     2101 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
     2161 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
     2221 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
     2281 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
     2341 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
     2401 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
     2461 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
     2521 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
     2581 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
     2641 gcaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
     2701 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
     2761 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
     2821 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
     2881 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
     2941 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
     3001 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
     3061 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
     3121 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
     3181 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
     3241 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
     3301 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
     3361 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
     3421 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
     3481 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
     3541 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
     3601 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
     3661 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
     3721 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
     3781 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
     3841 cggttgagat gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
     3901 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
     3961 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
     4021 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
     4081 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
     4141 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
     4201 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
     4261 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
     4321 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
     4381 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
     4441 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
     4501 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
     4561 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
     4621 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
     4681 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
     4741 ttaggacttg tgcgacatgt cgttttcaga agaaggctgc actgaacgtc agaagccgac
     4801 tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg
     4861 ttggcatgca catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg
     4921 attattctaa taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac
     4981 tgatagttta aaccagagaa gacaacagag cggagtctag ctcaacagag cttttaaccc
     5041 aaattggtac aatagaatac aactttagat cataattctc aaaagaaaga gattccttag
     5101 ctattctatc tgccactcca tttccttctc ggcttgtatg cacaagcata aaatcctcaa
     5161 acttgctaag tagatacttt atgtcttgga taattggatt gagacttgac aagcataact
     5221 ttcatgtaac caaagacaca agttgctgag aatccacctc aaaaatgatc ttcctataat
     5281 tgaatcggga taatgacagc acagcccatc taagagcctc cacttctact tccagcacgc
     5341 ttcttacttt taccacagct cttgcaccta accataacac cttccctgta tgatcgcgaa
     5401 gcacccaccc taagccacat tttaatcctt ctgttggcca tgccccatca aagttgcact
     5461 taacccaaga ttgtggtgga gcttcccatg tttctcgtct gtcccgacgg tgttgtggtt
     5521 ggtgctttcc ttacattctg agcctctttc cttctaatcc actcatctgc atcttcttgt
     5581 gtccttacta atacctcatt ggttccaaat tccctccctt taagcaccag ctcgtttctg
     5641 ttcttccaca gcctcccaag tatccaaggg actaaagcct ccacattctt cagatcagga
     5701 tattcttgtt taagatgttg aactctatgg aggtttgtat gaactgatga tctaggaccg
     5761 gataagttcc cttcttcata gcgaacttat tcaaagaatg ttttgtgtat cattcttgtt
     5821 acattgttat taatgaaaaa atattattgg tcattggact gaacacgagt gttaaatatg
     5881 gaccaggccc caaataagat ccattgatat atgaattaaa taacaagaat aaatcgagtc
     5941 accaaaccac ttgccttttt taacgagact tgttcaccaa cttgatacaa aagtcattat
     6001 cctatgcaaa tcaataatca tacaaaaata tccaataaca ctaaaaaatt aaaagaaatg
     6061 gataatttca caatatgtta tacgataaag aagttacttt tccaagaaat tcactgattt
     6121 tataagccca cttgcattag ataaatggca aaaaaaaaca aaaaggaaaa gaaataaagc
     6181 acgaagaatt ctagaaaata cgaaatacgc ttcaatgcag tgggacccac ggttcaatta
     6241 ttgccaattt tcagctccac cgtatattta aaaaataaaa cgataatgct aaaaaaatat
     6301 aaatcgtaac gatcgttaaa tctcaacggc tggatcttat gacgaccgtt agaaattgtg
     6361 gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg cagccggcac acacgagtcg
     6421 tgtttatcaa ctcaaagcac aaatactttt cctcaaccta aaaataaggc aattagccaa
     6481 aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca ttttattatt agctattgct
     6541 tcaccgcctt agctttctcg tgacctagtc gtcctcgtct tttcttcttc ttcttctata
     6601 aaacaatacc caaagagctc ttcttcttca caattcagat ttcaatttct caaaatctta
     6661 aaaactttct ctcaattctc tctaccgtga tcaaggtaaa tttctgtgtt ccttattctc
     6721 tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg tatatgttct ttggtttaga
     6781 ttctgttaat cttagatcga atacgatttt ctgggtttga tcgttagata tcatcttaat
     6841 tctcgattag ggtttcatag atatcatccg atttgttcaa ataatttgag ttttgtcgaa
     6901 taattactct tcgatttgtg atttctatct agatctggtg ttagtttcta gtttgtgcga
     6961 tcgaatttgt cgattaatct gagtttttct gattaacagt ctggaagcac catggagatg
     7021 gaagaaggag caagtggtgt tggtgagaag ataaagattg gagtctgcgt catggaaaag
     7081 aaggttttct cagctcccat gggggaaatt ctcgacagac tccagtcttt tggtgaattt
     7141 gagatcttac attttgggga taaagttata cttgaagatc caatagaaag ttggcccatt
     7201 tgtgattgct tgattgcttt tcattcctct ggatatcctc ttgagaaggc tcaggcatat
     7261 gctgctttaa gaaagccatt tttagtgaat gaacttgatc cgcaatatct tcttcatgat
     7321 cgccggaagg tgtatgagca tcttgagatg tatggcatcc cagttcctag gtatgcttgt
     7381 gtcaatagaa aggtaccaaa tcaagacctt cattattttg tcgaggaaga agattttgtt
     7441 gaggtccatg gtgaacgctt ctggaagcca tttgtggaaa agcctgtcaa tggagatgac
     7501 catagtataa tgatatacta ccctagctcg gcaggtggag gcatgaaaga attgtttcgc
     7561 aagattggga accgatcaag tgaatttcat cctgacgtca gaagggtaag gagagaaggc
     7621 tcttatatat acgaggagtt tatggccacc ggaggaactg atgtcaaggt ctatacagtg
     7681 ggtcctgaat acgcacatgc tgaagcaaga aagtcacctg ttgttgatgg tgttgttatg
     7741 aggaatacag atgggaagga agtgagatat ccagtattgc ttacacctgc tgaaaagcaa
     7801 atggctagag aagtttgcat tgcatttagg caagcggtct gtgggtttga tcttctacga
     7861 tctgagggct gttcatatgt ttgtgatgtc aatggatgga gttttgtgaa gaactcatac
     7921 aagtattatg acgatgctgc gtgtgtgcta aggaaaatgt gtttggatgc aaaagctcct
     7981 catctctcat ccactcttcc tcccaccttg ccatggaagg tcaatgaacc tgtacaatct
     8041 aatgaaggtc taactcgcca gggaagtggc atcatcggca cttttgggca gtcagaagag
     8101 ctacgttgtg tcattgctgt tgttcgacat ggcgatagaa ctcccaagca gaaagtgaaa
     8161 ctaaaagtta cagaggaaaa actattaaac ctaatgttga agtacaatgg tggaaagcca
     8221 agagctgaaa ccaaacttaa gagtgccgtc cagttgcaag atctattaga tgccacaaga
     8281 atgttagttc cccgtacaag accaggtcgt gaaagtgata gtgatgcaga ggaccttgaa
     8341 catgctgaga agcttcggca agttaaagca gtccttgaag agggaggaca tttctctggt
     8401 atatacagga aggttcaact gaagccgctg aagtgggtta aaataccaaa aagcgatggt
     8461 gatggcgaag aagaaagacc agtagaggcc cttatggttc tgaaatatgg cggtgtttta
     8521 acacacgctg gtagaaagca ggcagaagaa cttggtagat actttcgaaa caatatgtat
     8581 ccaggtgaag ggactggttt gcttcgtctc catagtacgt accgtcatga ccttaaaatt
     8641 tacagctctg acgagggacg tgttcagatg tctgcagcgg cttttgctaa aggcctgctt
     8701 gacctagaag gacagctgac gccaatcctg gtttctttgg ttagcaagga ctcttccatg
     8761 ttggatggtc ttgataatgc cagcattgaa atggaagcgg ccaaggctag attgaatgag
     8821 attgtaacgt ctggcacaaa gatgatagac gacgaccaag tctcctctga agatttccct
     8881 tggatgactg atggagctgg acttcctccc aacgctcatg aactcctacg tgaactggtg
     8941 aaattaacta agaatgtgac tgaacaagta agactacttg caatggatga ggacgagaac
     9001 ctcactgagc catacgatat aattcctcca tatgatcaag caaaagccct tggcaagaca
     9061 aacattgaca gtgatcggat tgcttctgga ttaccatgtg gtagtgaagg attccttctg
     9121 atgtttgctc ggtggataaa acttgctagg gatctctaca atgaaagaaa agaccgattt
     9181 gacatcacac agattccgga tgtttacgat tcatgcaagt acgacctgtt acataattcc
     9241 catctcgatc taaaaggatt agatgaactc ttcaaagtag cacagttact tgcagatggt
     9301 gtaatcccaa atgagtatgg catcaatccg caacaaaagc ttaaaatcgg ttcaaagatt
     9361 gctcggcgct taatggggaa aatcttgata gacttgagga atactcgaga agaagcactg
     9421 agcgttgccg agttgaaaga aagccaagaa caagtcttgt cattatccgc ctcgcaaagg
     9481 gaagatagaa atagtcaacc gaagctattt atcaatagcg atgaattgag acgacctggc
     9541 acaggtgata aagatgaaga tgatgataaa gaaaccaaat accgattaga tccaaagtat
     9601 gcaaatgtga aaacacctga acgtcatgtg aggactcgac tttacttcac atctgaatcg
     9661 catattcatt cgctcatgaa cgtccttcga tactgtaacc ttgatgaatc tctcctagga
     9721 gaagaaagcc tcatttgcca aaacgcctta gaacgtctct gtaaaaccaa ggaactcgat
     9781 tacatgagtt acattgtcct aaggctcttt gagaacaccg aggtatcact tgaggacccg
     9841 aaaagattcc gcattgaact tacatttagc cgtggagctg atttgtctcc cttaaggaat
     9901 aatgacgacg aggcagagac attgctaagg gaacacacac ttccgataat gggaccagag
     9961 aggcttcaag aagtaggttc ttgtttaagc ttggagacaa tggagaaaat ggttcgtcca
    10021 tttgctatgc cggcggaaga tttccctccg gcgtccactc cggttggttt ctcgggttac
    10081 ttctcaaaaa gtgctgcggt gctggagcga ctcgtaaacc tcttccacaa ctataagaac
    10141 tcttcttcta atggaaggag caagggagat tacaaagacc atgatggaga ttataaggat
    10201 cacgatatcg actacaaaga tgacgacgat aaataaaatc gatcgttcaa acatttggca
    10261 ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct
    10321 gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg
    10381 ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata
    10441 gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag atctgaggta
    10501 tgtaggtata gtaacatgaa caagaacaga ctcaagtaca agatcgcata cgaaaatgga
    10561 aatggcaatg tcacttccac ataatcaaac acgaatcctc atatcaacaa ggcctgagat
    10621 tctaactagc tcataacaac ttagccaata gttacttgag actaccaaat gtatgtagaa
    10681 ctaaagacta agggacagag agttcgtcta aacaggtgaa tctagtcgtt gttatctaat
    10741 aaacaattca gccccaaatg cagaacacac atagagctct ctattgattc aaattacgat
    10801 ctgatactga taacgtctag atttttaggg ttaaagcaat caatcacctg acgattcaag
    10861 gtggttggat catgacgatt ccagaaaaca tcaagcaagc tctcaaagct acactctttg
    10921 ggatcatact gaactctaac aacctcgtta tgtcccgtag tgccagtaca gacatcctcg
    10981 taactcggat tgtgcacgat gccatggcta tacccaacct cggtcttggt cacaccagga
    11041 actctctggt aagctagctc cactccccag aaacaaccgg cgccaaattg cgcgaattgc
    11101 tgacctgatg acggaacatc atcgtcgggt ccttgggcga ttgcggcgga agatgggtca
    11161 gcttgggctt gaggacgaga gccgaatccg agtctgttga aaaggttgtt cattggggat
    11221 ttgtatacgg agattggtcg tcgagaggtt tgagggaaag gacaaatggg tttggctctg
    11281 gagaaagaga gtgcggcttt agagagagaa ttgagaggtt tagagagaga tgcggcggcg
    11341 atgagcgtgc cgaggagaga cgacgaggac ctgcattatc aaagcagtga cgtggtgaaa
    11401 tttggaactt ttaagaggca gatagattta ttatttgtat ccattttctt cattgttcta
    11461 gaatgtcgcg gaacaaattt taaaactaaa tcctaaattt ttctaatttt gttgccaata
    11521 gtggatatgt gggccgtata gaaggaatct attgaaggct caaacccata ctgacgagcc
    11581 caaaggttcg ttttgcgttt tatgtttcgg ttcgatgcca acgccacatt ctgagctagg
    11641 caaaaaacaa acgtgtcttt gaatagactc ctctcgttaa cacatgcagc ggctgcatgg
    11701 tgacgccatt aacacgtggc ctacaattgc atgatgtctc cattgacacg tgacttctcg
    11761 tctcctttct taatatatct aacaaacact cctacctctt ccaaaatata tacacatctt
    11821 tttgatcaat ctctcattca aaatctcatt ctctctagta aacaagaaca aaaaaatggc
    11881 ggatacagct agaggaaccc atcacgatat catcggcagg gaccagtacc cgatgatggg
    11941 ccgtgaccgt gaccagtacc agatgtccgg acgaggatct gactactcca agtctaggca
    12001 gattgctaaa gctgcaactg ctgtcacagc tggtggttcc ctccttgttc tctccagcct
    12061 tacccttgtt ggaactgtca tagctttgac tgttgcaaca cctctgctcg ttatcttcag
    12121 cccaatcctt gtcccggctc tcatcacagt tgcactcctc atcaccggtt ttctttcctc
    12181 tggagggttt ggcattgccg ctataaccgt tttctcttgg atttacaagt aagcacacat
    12241 ttatcatctt acttcataat tttgtgcaat atgtgcatgc atgtgttgag ccagtagctt
    12301 tggatcaatt tttttggtcg aataacaaat gtaacaataa gaaattgcaa attctaggga
    12361 acatttggtt aactaaatac gaaatttgac ctagctagct tgaatgtgtc tgtgtatatc
    12421 atctatatag gtaaaatgct tggtatgata cctattgatt gtgaataggt acgcaacggg
    12481 agagcaccca cagggatcag acaagttgga cagtgcaagg atgaagttgg gaagcaaagc
    12541 tcaggatctg aaagacagag ctcagtacta cggacagcaa catactggtg gggaacatga
    12601 ccgtgaccgt actcgtggtg gccagcacac tactatgagc gagctgatta aggagaacat
    12661 gcacatgaag ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga
    12721 gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg
    12781 ccctctcccc ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt
    12841 catcaaccac acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac
    12901 atgggagaga gtcaccacat acgaggacgg aggtgtgctg accgctactc aggacaccag
    12961 cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa
    13021 cggccctgtg atgcagaaga aaacactcgg ctgggaagcc aacaccgaga tgctgtatcc
    13081 agctgacgga ggactggaag gcagaagcga catggccctg aagctcgtgg gcgggggcca
    13141 cctgatctgc aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat
    13201 gcccggcgtc tactatgtgg accacagact ggaaagaatc aaggaggccg acaaagaaac
    13261 ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg
    13321 gcacaagtga ttagagtttt tcagatccgc gtttgtgttt tctgggtttc tcacttaagc
    13381 gtctgcgttt tacttttgta ttgggtttgg cgtttagtag tttgcggtag cgttcttgtt
    13441 atgtgtaatt acgctttttc ttcttgcttc agcagtttcg gttgaaatat aaatcgaatc
    13501 aagtttcact ttatcagcgt tgttttaaat tttggcatta aattggtgaa aattgcttca
    13561 attttgtatc taaatagaag tgtcctcatt gtcttccgta atccgtctaa gcgtcaattt
    13621 gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc ggcagctcgg
    13681 cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca gcgggagagc
    13741 cgttgtaagg cggcagactt tgctcatgtt accggcagaa gatcctttga tcttttctac
    13801 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcatgatat
    13861 atctcccaat ttgtgtaggg cttattatgc acgcttaaaa ataataaaag cagacttgac
    13921 ctgatagttt ggctgtgagc aattatgtgc ttagtgcatc taacgcttga gttaagccgc
    13981 gccgcgaagc ggcgtcggct tgaacgaatt tctagctaga cattatttgc cgactacctt
    14041 ggtgatctcg cctttcacgt agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa
    14101 gcgatcttct tcttgtccaa gataagcctg tctagcttca agtatgacgg gctgatactg
    14161 ggccggcagg cgctccattg cccagtcggc agcgacatcc ttcggcgcga ttttgccggt
    14221 tactgcgctg taccaaatgc gggacaacgt aagcactaca tttcgctcat cgccagccca
    14281 gtcgggcggc gagttccata gcgttaaggt ttcatttagc gcctcaaata gatcctgttc
    14341 aggaaccgga tcaaagagtt cctccgccgc tggacctacc aaggcaacgc tatgttctct
    14401 tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg gctggctcga agatacctgc
    14461 aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg cgcttagctg gataacgcca
    14521 cggaatgatg tcgtcgtgca caacaatggt gacttctaca gcgcggagaa tctcgctctc
    14581 tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc
    14641 aagccttacg gtcaccgtaa ccagcaaatc aatatcactg tgtggcttca ggccgccatc
    14701 cactgcggag ccgtacaaat gtacggccag caacgtcggt tcgagatggc gctcgatgac
    14761 gccaactacc tctgatagtt gagtcgatac ttcggcgatc accgcttccc ccatgatgtt
    14821 taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
    14881 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
    14941 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
    15001 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
    15061 cgaacaggct tatgtccact gggttcgtgc ccgaattgat cacaggcagc aacgctctgt
    15121 catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct
    15181 tagttgccgt tcttccgatg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
