LOCUS       sequence-475937-        2502 bp    DNA     circular SYN 10-JUL-2025
DEFINITION  BsaI Golden Gate assembly plasmid encoding the tagBFP CDS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Peterman EL, Ploessl DS, Love KS, Sanabria V, Daniels RF, Johnstone 
            CP, Godavarti DR, Kabaria SR, Oakes CG, Pai AA, Galloway KE
  TITLE     High-resolution profiling reveals coupled transcriptional and 
            translational regulation of transgenes.
  JOURNAL   Nucleic Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528
  PUBMED    39651241
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528"
COMMENT     Sequence Label: pKG1055_pPV2-tagBFP
FEATURES             Location/Qualifiers
     source          1..2502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      33..621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     522..541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             653..1354
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        1399..1503
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1504..1572,1573..2364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1722..1741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
       61 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      121 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      181 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      241 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      301 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      361 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      421 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
      481 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
      541 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
      601 ggggggcgga gcctatggaa aaacgccagc aacgggtctc aaatgagcca ccatgagcga
      661 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
      721 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
      781 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
      841 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
      901 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
      961 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     1021 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
     1081 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
     1141 gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc
     1201 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
     1261 ggaggccaac aacgaaacgt acgtcgagca gcacgaggtg gcagtggcca gatactgcga
     1321 cctccctagc aaactggggc acaagcttaa ttaacaacag agacctagac gtcaggtggc
     1381 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     1441 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     1501 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     1561 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     1621 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     1681 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     1741 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     1801 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     1861 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     1921 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     1981 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     2041 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     2101 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     2161 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     2221 tatcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     2281 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     2341 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     2401 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     2461 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cg
//
