LOCUS       sequence-475942-        3005 bp    DNA     circular SYN 10-JUL-2025
DEFINITION  BsaI Golden Gate assemby plasmid encoding the UbC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Peterman EL, Ploessl DS, Love KS, Sanabria V, Daniels RF, Johnstone 
            CP, Godavarti DR, Kabaria SR, Oakes CG, Pai AA, Galloway KE
  TITLE     High-resolution profiling reveals coupled transcriptional and 
            translational regulation of transgenes.
  JOURNAL   Nucleic Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528
  PUBMED    39651241
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 10, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Research, Volume 53, Issue 11, 24 June 2025, gkaf528"
COMMENT     Sequence Label: pKG1988_pPV1-UbC
FEATURES             Location/Qualifiers
     source          1..3005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     458..477
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        582..1793
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1630..1649
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     promoter        1838..1942
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1943..2011,2012..2803)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGYRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2161..2180)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(2974..3005,1..557)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
       61 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
      121 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
      181 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
      241 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
      301 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
      361 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
      421 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
      481 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
      541 ggcggagcct atggaaaaac gccagcaacg ggtctcatac tggcctccgc gccgggtttt
      601 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
      661 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
      721 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
      781 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
      841 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
      901 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
      961 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     1021 gccgggccgc tcggtgggac ggaagcgtgt ggagagatcg ccaagggctg tagtctgggt
     1081 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     1141 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     1201 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     1261 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     1321 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     1381 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     1441 tggcttataa tgcagggtgg ggccacctgt cggtaggtgt gcggtaggct tttctccgtc
     1501 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     1561 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     1621 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     1681 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     1741 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacaatgaga
     1801 gacctagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1861 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1921 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1981 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     2041 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     2101 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     2161 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     2221 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     2281 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     2341 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     2401 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     2461 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     2521 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     2581 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     2641 tggagccggt gagcgtgggt atcgcggtat cattgcagca ctggggccag atggtaagcc
     2701 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     2761 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2821 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2881 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2941 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     3001 ctgct
//
