LOCUS       sequence-476067-        8190 bp    DNA     circular SYN 13-JUN-2025
DEFINITION  To express GFP from a 0.5 bp synapsin promoter. The non-coding BDNF 
            exon one is 5' to the BDNF start codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8190)
  TITLE     Ploski Lab Plasmids 2025
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 13, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti-0.5Syn-Exon 1 of BNDF-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..8190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    87..212
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             259..439
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(440..666)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(694..714)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(694..712)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(733..749)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(733..749)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(746..768)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    757..773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(781..787,788..805,806..811))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    826..847
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(964..981)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1135..1723)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1215..1234)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1894..2685,2686..2754))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2517..2536
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2755..2859)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2885..3340)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3022..3043)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3234..3253
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3467..3489
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3481..3498
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3482..3498
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3508..3527
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3508..3526
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3547..3682
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3722..3843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3733..3752)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3787..3806
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3915..4148
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4228..4816
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4334..4345
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4743..4763
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4840..4881)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    4934..4958
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(4959..5672,5673..5675,5676..5678))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(4962..5672,5673..5675,5676..5678))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5004..5025)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5353..5372
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5612..5633
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5675..5684
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(5687..5711)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6938..7055
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7322..7366)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7551..7784
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 atatgtttta atttatattt tttctttccc cctggcctta accgaatttt ttcccatcgc
       61 gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc cttctagcct
      121 ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc
      181 gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt cgctttcaag
      241 tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt ctgagggatc
      301 tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact caaggcaagc
      361 tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag gctcagatct
      421 ggtctaacca gagagacccg tttatgtatc gagctaggca cttaaataca atatctctgc
      481 aatgcggcaa ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc cttcctaata
      541 aggcacgatc gtaccacctt acttccacca atcggcatgc acggtgcttt ttctctcctt
      601 gtaaggcatg ttgctaactc atcgttacca tgttgcaaga ctacaagagt attgcataag
      661 actacattaa gcttgcagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
      721 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
      781 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      841 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      901 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      961 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1021 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1081 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1141 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1201 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1261 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1321 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1381 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     1441 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     1501 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     1561 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     1621 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     1681 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1741 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1801 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1861 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1921 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1981 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2041 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2101 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2161 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2221 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2281 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2341 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2401 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2461 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2521 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     2581 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     2641 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     2701 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2761 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2821 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2881 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     2941 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     3001 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     3061 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     3121 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     3181 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     3241 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     3301 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     3361 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     3421 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     3481 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacg
     3541 tccctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg
     3601 cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac
     3661 tgggcggagt taggggcggg atgggcggag ttaggggcgg gatagctaga gccagacatg
     3721 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     3781 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     3841 gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa taataagatg
     3901 acatgaacta ctactgctag agattttcca cactgactaa aagggtctga gggatctcta
     3961 gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag gcaagcttta
     4021 ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc agatctggtc
     4081 taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg agtgaattag
     4141 cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg ccttgtaagt
     4201 cattggtctt aaaggtacca tcgccaggcg gggaggcggc ccaaagggag atccgactcg
     4261 tctgagggcg aaggcgaaga cgcggaagag gccgcagagc cggcagcagg ccgcgggaag
     4321 gaaggtccgc tggattgagg gccgaaggga cgtagcagaa ggacgtcccg cgcagaatcc
     4381 aggtggcaac acaggcgagc agccatggaa aggacgtcag cttccccgac aacaccacgg
     4441 aattgtcagt gcccaacagc cgagcccctg tccagcagcg ggcaaggcag gcggcgatga
     4501 gttccgccgt ggcaataggg agggggaaag cgaaagtccc ggaaaggagc tgacaggtgg
     4561 tggcaatgcc ccaaccagtg ggggttgcgt cagcaaacac agtgcacacc acgccacgtt
     4621 gcctgacaac gggccacaac tcctcataaa gagacagcaa ccaggattta tacaaggagg
     4681 agaaaatgaa agccatacgg gaagcaatag catgatacaa aggcattaaa gcagcgtatc
     4741 cacatagcgt aaaaggagca acatagttaa gaataccagt caatctttca caaattttgt
     4801 aatccagagg ttgattgtcg atcattacta accggtacgc gtagaatcga gaccgaggag
     4861 agggttaggg ataggcttac cttcgaaccg cgggccctct agactcgagc ggccgccact
     4921 gtgctggata tcaaccactt tgtacaagaa agctgggttt acttgtacag ctcgtccatg
     4981 ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg
     5041 ttggggtctt tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg
     5101 gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc
     5161 tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg
     5221 atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc
     5281 tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc
     5341 acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg
     5401 tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg
     5461 ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc
     5521 ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc
     5581 tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc
     5641 accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcgtagcc tgcttttttg
     5701 tacaaacttg ttgatatctg cagaattcca ccacactgga ctagttgtgg ctttgctgtc
     5761 ctggagactc agtgtcttaa aatcttgtct ccccaacaga tgcaggaagg taatgtgtct
     5821 tgtttgaagt catcatgtga gaagttcggc tttgctcagt ggatcgccca ccacttggtg
     5881 tgacttatga atctaataac ccttcgcaat atccgcaaag cttcaactct catccacctt
     5941 ggcgactaca gaagacaaag caactggcat cgatgttgaa aaacctatag atttacgcaa
     6001 acgccctcat tctgagagac acgtttccct ttgagttctt acgtgattct aatgaatgag
     6061 cgaggttacc aatgactgtc caattgccct gctatatagc gggcgtttcc tgaagcagct
     6121 ttctcaacgc ctgtcactga gccctagcac aaaaagttcc cagagaaagt gagggagttt
     6181 cagagtaccc ccataaaaat aatccaattt gcacgccgat cctttgccca ctgctcaggt
     6241 cacacctggg gctctggcaa agatgagctc ggctcaggag atttccctgt cgccaggtaa
     6301 gaaacccttc gccttgtcaa gctagggcgg gaagaccgct ggggaacttg ttgcttttct
     6361 gcaccagccg gctactgctt taactagtgg atccaagggg cagtgggtcg gtgggccgcg
     6421 ggggcggagc gcaaggggct caggagcacg gcggcgctct ctggcacgac gcgactcctc
     6481 tgctgcccgc tgcagactga ggcagcgctg aggctctgcc gccgcagagc gtatggtcgc
     6541 gcctgcaccc ccctatcttg cacctcttgt ggtgcggccc gactggactc accgctgcac
     6601 ggacgcgacc aaggcggtga ggtaggggag tttggggagg gctggagagt gacgtcagcc
     6661 cgcctttggt gctggggagg tggtggcgcg cgccggcagc gggggcgaag gctctgtccg
     6721 cggtactaaa tccgacagtg cgcacgcgcc gcgccgcgct gcatcctctt cccctccccc
     6781 tctgatgggg gagggaatgc gcaatttggg gagtgggagt gggggttctt gtccgtaggg
     6841 tctggacccg tagtaaggct cctactctgt ttttcagcct cctcctaaat cctcaggcta
     6901 acttgcattg ttacccttcc cagatacttg tatcgataaa attttgaatt tttgtaattt
     6961 gtttttgtaa ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg
     7021 cactgtaccc cccaatcccc ccttttcttt taaaagttaa ccgataccgt cgagatccgt
     7081 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
     7141 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
     7201 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
     7261 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
     7321 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
     7381 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
     7441 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
     7501 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
     7561 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
     7621 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
     7681 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
     7741 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
     7801 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
     7861 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
     7921 gtctgaagat cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct
     7981 atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg
     8041 gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt
     8101 agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg
     8161 aatcgttcta gctccctgct tgcccatact
//
