LOCUS       sequence-476167-        3756 bp    DNA     circular SYN 16-JUN-2025
DEFINITION  Firefly luciferase (red-shifted), codon optimized for Chlamydomonas 
            reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_4_033_Firefly_luciferase
FEATURES             Location/Qualifiers
     source          1..3756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      1700..1757
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(1781..1798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1952..2540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2032..2051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2723..2817)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2838..3497)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     3412..3431
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     3659..3677
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(3681..3724)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    3727..3748
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcgaat ggaagatgct aaaaatatta aaaaaggtcc agctccattt tatccattag
       61 aagatggtac agctggtgaa caattacata aagctatgaa acgttatgct ttagttccag
      121 gtacaattgc ttttacagat gctcatattg aagttgatat tacatatgct gaatattttg
      181 aaatgtcagt tcgtttagct gaagctatga aacgttatgg tttaaataca aatcatcgta
      241 ttgttgtttg ttcagaaaat tcattacaat tttttatgcc agttttaggt gctttattta
      301 ttggtgttgc tgttgctcca gctaatgata tttataatga acgtgaatta ttaaattcaa
      361 tgggtatttc acaaccaaca gttgtttttg tttcaaaaaa aggtttacaa aaaattttaa
      421 atgttcaaaa aaaattacca attattcaaa aaattattat tatggattca aaaacagatt
      481 atcaaggttt tcaatcaatg tatacatttg ttacatcaca tttaccacca ggttttaatg
      541 aatatgattt tgttccagaa tcatttgatc gtgataaaac aattgcttta attatgaatt
      601 catcaggttc aacaggttta ccaaaaggtg ttgctttacc acatcgtaca gcttgtgttc
      661 gtttttcaca tgctcgtgat ccaatttttg gtaatcaaat tattccagat acagctattt
      721 tatcagttgt tccatttcat catggttttg gtatgtttac aacattaggt tatttaattt
      781 gtggttttcg tgttgtttta atgtatcgtt ttgaagaaga attattttta cgttcattac
      841 aagattataa aattcaatca gctttattag ttccaacatt attttcattt tttgctaaat
      901 caacattaat tgataaatat gatttatcaa atttacatga aattgcttca ggtggtgctc
      961 cattatcaaa agaagttggt gaagctgttg ctaaacgttt tcatttacca ggtattcgtc
     1021 aaggttatgg tttaacagaa acaacatcag ctattttaat tacaccagaa ggtgatgata
     1081 aaccaggtgc tgttggtaaa gttgttccat tttttgaagc taaagttgtt gatttagata
     1141 caggtaaaac attaggtgtt aatcaacgtg gtgaattatg tgttcgtggt ccaatgatta
     1201 tgtcaggtta tgttaataat ccagaagcta caaatgcttt aattgataaa gatggttggt
     1261 tacattcagg tgatattgct tattgggatg aagatgaaca tttttttatt gttgatcgtt
     1321 taaaatcatt aattaaatat aaaggttatc aagttgctcc agctgaatta gaatcaattt
     1381 tattacaaca tccaaatatt tttgatgctg gtgttgctgg tttaccagat gatgatgctg
     1441 gtgaattacc agctgctgtt gttgttttag aacatggtaa aacaatgaca gaaaaagaaa
     1501 ttgttgatta tgttgcttca caagttacaa cagctaaaaa attacgtggt ggtgttgttt
     1561 ttgttgatga agttccaaaa ggtttaacag gtaaattaga tgctcgtaaa attcgtgaaa
     1621 ttttaattaa agctaaaaaa ggtggtaaaa ttgctgttgc tttgagacca ctcatcgcga
     1681 ctagtagcgg ccgctgcagt ccggcaaaaa agggcaaggt gtcaccaccc tgcccttttt
     1741 ctttaaaacc gaaaagatta cttcgcgtta tgcaggcttc ctcgctcact gactcgctgc
     1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc cctgacgagc
     1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     2701 ggtctgacag ctcgaggctt ggattctcac caataaaaaa cgcccggcgg caaccgagcg
     2761 ttctgaacaa atccagatgg agttctgagg tcattactgg atctatcaac aggagtccaa
     2821 gcgagctcga tatcaaatta cgccccgccc tgccactcgt cgcagtactg ttgtaattca
     2881 ttaagcattc tgccgacatg gaagccatca caaacggcat gatgaacctg aatcgccagc
     2941 ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca tggtgaaaac gggggcgaag
     3001 aagttgtcca tattggccac gtttaaatca aaactggtga aactcaccca gggattggct
     3061 gatacgaaaa acatattctc aataaaccct ttagggaaat aggccaggtt ttcaccgtaa
     3121 cacgccacat cttgcgaata tatgtgtaga aactgccgga aatcgtcgtg gtattcactc
     3181 cagagtgatg aaaacgtttc agtttgctca tggaaaacgg tgtaacaagg gtgaacacta
     3241 tcccatatca ccagctcacc gtctttcatt gccatacgaa attccggatg agcattcatc
     3301 aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt gcttattttt ctttacggtc
     3361 tttaaaaagg ccgtaatatc cagctgaacg gtctggttat aggtacattg agcaactgac
     3421 tgaaatgcct caaaatgttc tttacgatgc cattgggata tatcaacggt ggtatatcca
     3481 gtgatttttt tctccatttt agcttcctta gctcctgaaa atctcgataa ctcaaaaaat
     3541 acgcccggta gtgatcttat ttcattatgg tgaaagttgg aacctcttac gtgcccgatc
     3601 aactcgagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     3661 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
     3721 tttctggaat tcgcggccgc ttctagagcg atgagt
//
