LOCUS       sequence-476320-        7400 bp    DNA     circular SYN 18-JUN-2025
DEFINITION  AAV packaging plasmid expressing AAV2 Rep protein and AAV11 capsid 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Addgene Research Program
  TITLE     Addgene AAV RepCap Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV2/11 v1
FEATURES             Location/Qualifiers
     source          1..7400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             2606..2614
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(4031..4047)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4031..4047)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4263..4283)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4263..4281)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4302..4318)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4302..4318)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4315..4337)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4326..4342
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4350..4356,4357..4374,4375..4380))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4395..4416
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4533..4550)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4704..5292)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4784..4803)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5463..6254,6255..6323))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6086..6105
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6324..6428)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6454..6909)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6591..6612)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6803..6822
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7036..7058
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7050..7067
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7051..7067
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7077..7096
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7077..7095
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     7120..7136
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     7121..7137
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gagtaaggcc ccggaggctc ttttctttgt gcaatttgag aagggagaga gctacttcca
       61 catgcacgtg ctcgtggaaa ccaccggggt gaaatccatg gttttgggac gtttcctgag
      121 tcagattcgc gaaaaactga ttcagagaat ttaccgcggg atcgagccga ctttgccaaa
      181 ctggttcgcg gtcacaaaga ccagaaatgg cgccggaggc gggaacaagg tggtggatga
      241 gtgctacatc cccaattact tgctccccaa aacccagcct gagctccagt gggcgtggac
      301 taatatggaa cagtatttaa gcgcctgttt gaatctcacg gagcgtaaac ggttggtggc
      361 gcagcatctg acgcacgtgt cgcagacgca ggagcagaac aaagagaatc agaatcccaa
      421 ttctgatgcg ccggtgatca gatcaaaaac ttcagccagg tacatggagc tggtcgggtg
      481 gctcgtggac aaggggatta cctcggagaa gcagtggatc caggaggacc aggcctcata
      541 catctccttc aatgcggcct ccaactcgcg gtcccaaatc aaggctgcct tggacaatgc
      601 gggaaagatt atgagcctga ctaaaaccgc ccccgactac ctggtgggcc agcagcccgt
      661 ggaggacatt tccagcaatc ggatttataa aattttggaa ctaaacgggt acgatcccca
      721 atatgcggct tccgtctttc tgggatgggc cacgaaaaag ttcggcaaga ggaacaccat
      781 ctggctgttt gggcctgcaa ctaccgggaa gaccaacatc gcggaggcca tagcccacac
      841 tgtgcccttc tacgggtgcg taaactggac caatgagaac tttcccttca acgactgtgt
      901 cgacaagatg gtgatctggt gggaggaggg gaagatgacc gccaaggtcg tggagtcggc
      961 caaagccatt ctcggaggaa gcaaggtgcg cgtggaccag aaatgcaagt cctcggccca
     1021 gatagacccg actcccgtga tcgtcacctc caacaccaac atgtgcgccg tgattgacgg
     1081 gaactcaacg accttcgaac accagcagcc gttgcaagac cggatgttca aatttgaact
     1141 cacccgccgt ctggatcatg actttgggaa ggtcaccaag caggaagtca aagacttttt
     1201 ccggtgggca aaggatcacg tggttgaggt ggagcatgaa ttctacgtca aaaagggtgg
     1261 agccaagaaa agacccgccc ccagtgacgc agatataagt gagcccaaac gggtgcgcga
     1321 gtcagttgcg cagccatcga cgtcagacgc ggaagcttcg atcaactacg cagacaggta
     1381 ccaaaacaaa tgttctcgtc acgtgggcat gaatctgatg ctgtttccct gcagacaatg
     1441 cgagagaatg aatcagaatt caaatatctg cttcactcac ggacagaaag actgtttaga
     1501 gtgctttccc gtgtcagaat ctcaacccgt ttctgtcgtc aaaaaggcgt atcagaaact
     1561 gtgctacatt catcatatca tgggaaaggt gccagacgct tgcactgcct gcgatctggt
     1621 caatgtggat ttggatgact gcatctttga acaataaatg atttaaatca ggtatggctg
     1681 ctgacggtta tcttccagat tggctcgagg acaacctctc tgagggcatt cgcgagtggt
     1741 gggacctgaa acctggagcc ccgaagccca aggccaacca gcagaagcag gacgacggcc
     1801 ggggtctggt gcttcctggc tacaagtacc tcggaccctt caacggactc gacaaggggg
     1861 agcccgtcaa cgcggcggac gcagcggccc tcgagcacga caaggcctac gaccagcagc
     1921 tcaaagcggg tgacaatccg tacctgcggt ataaccacgc cgacgccgag tttcaggagc
     1981 gtctgcaaga agatacgtct tttgggggca acctcgggcg agcagtcttc caggccaaga
     2041 agagggtact cgaacctctg ggcctggttg aagaaggtgc taaaacggct cctggaaaga
     2101 agagaccgtt agagtcacca caagagcccg actcctcctc gggcatcggc aaaaaaggca
     2161 aacaaccagc cagaaagagg ctcaactttg aagaggacac tggagccgga gacggacccc
     2221 ctgaaggatc agataccagc gccatgtctt cagacattga aatgcgtgca gcaccgggcg
     2281 gaaatgctgt cgatgcggga caaggttccg atggagtggg taatgcctcg ggtgattggc
     2341 attgcgattc cacctggtct gagggcaagg tcacaacaac ctcgaccaga acctgggtct
     2401 tgcccaccta caacaaccac ttgtacctgc gtctcggaac aacatcaagc agcaacacct
     2461 acaacggatt ctccaccccc tggggatatt ttgacttcaa cagattccac tgtcacttct
     2521 caccacgtga ctggcaaaga ctcatcaaca acaactgggg actacgacca aaagccatgc
     2581 gcgttaaaat cttcaatatc caagttaagg aggtcacaac gtcgaacggc gagactacgg
     2641 tcgctaataa ccttaccagc acggttcaga tatttgcgga ctcgtcgtat gagctcccgt
     2701 acgtgatgga cgctggacaa gaggggagcc tgcctccttt ccccaatgac gtgttcatgg
     2761 tgcctcaata tggctactgt ggcatcgtga ctggcgagaa tcagaaccaa acggacagaa
     2821 acgctttcta ctgcctggag tattttcctt cgcaaatgtt gagaactggc aacaactttg
     2881 aaatggctta caactttgag aaggtgccgt tccactcaat gtatgctcac agccagagcc
     2941 tggacagact gatgaatccc ctcctggacc agtacctgtg gcacttacag tcgactacct
     3001 ctggagagac tctgaatcaa ggcaatgcag caaccacatt tggaaaaatc aggagtggag
     3061 actttgcctt ttacagaaag aactggctgc ctgggccttg tgttaaacag cagagattct
     3121 caaaaactgc cagtcaaaat tacaagattc ctgccagcgg gggcaacgct ctgttaaagt
     3181 atgacaccca ctatacctta aacaaccgct ggagcaacat cgcgcccgga cctccaatgg
     3241 ccacagccgg accttcggat ggggacttca gtaacgccca gcttatattc cctggaccat
     3301 ctgttaccgg aaatacaaca acttcagcca acaatctgtt gtttacatca gaagaagaaa
     3361 ttgctgccac caacccaaga gacacggaca tgtttggcca gattgctgac aataatcaga
     3421 atgctacaac tgctcccata accggcaacg tgactgctat gggagtgctg cctggcatgg
     3481 tgtggcaaaa cagagacatt tactaccaag ggccaatttg ggccaagatc ccacacgcgg
     3541 acggacattt tcatccttca ccgctgattg gtgggtttgg actgaaacac ccgcctcccc
     3601 agatattcat caagaacact cccgtacctg ccaatcctgc gacaaccttc actgcagcca
     3661 gagtggactc tttcatcaca caatacagca ccggccaggt cgctgttcag attgaatggg
     3721 aaattgaaaa ggaacgctcc aaacgctgga atcctgaagt gcagtttact tcaaactatg
     3781 ggaaccagtc ttctatgttg tgggctcctg atacaactgg gaagtataca gagccgcggg
     3841 ttattggctc tcgttatttg actaatcatt tgtaattacg tgttaatcaa taaaccggtt
     3901 gattcgtttc agttgaactt tggtctcctg tccttcttat cttatcggtt accatggtta
     3961 tagcttacac attaactgct tggttgcgct tcgcgataaa agacttacgt catcgggtta
     4021 cccctagggg gatccactag ttctagaggt cctgtattag aggtcacgtg agtgttttgc
     4081 gacattttgc gacaccatgt ggtcacgctg ggtatttaag cccgagtgag cacgcagggt
     4141 ctccattttg aagcgggagg tttgaacgcg cagccgccaa gccgaattct gcagatatcc
     4201 atcacactgg cggccgctcg actagagcgg ccgccaccgc ggtggagctc cagcttttgt
     4261 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
     4321 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4381 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4441 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4501 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4561 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4621 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4681 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4741 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4801 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4861 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4921 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4981 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5041 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5101 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5161 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5221 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5341 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5401 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5461 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5521 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5581 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5761 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5821 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5881 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5941 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6001 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6061 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6121 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6181 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6241 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6301 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6421 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     6481 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     6541 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     6601 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     6661 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     6721 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6781 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6841 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6901 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     6961 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     7021 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     7081 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgggg
     7141 gagctcgcag ggtctccatt ttgaagcggg aggtttgaac gcgcagccgc catgccgggg
     7201 ttttacgaga ttgtgattaa ggtccccagc gaccttgacg agcatctgcc cggcatttct
     7261 gacagctttg tgaactgggt ggccgagaag gaatgggagt tgccgccaga ttctgacatg
     7321 gatctgaatc tgattgagca ggcacccctg accgtggccg agaagctgca gcgcgacttt
     7381 ctgacggaat ggcgccgtgt
//
