LOCUS       sequence-476349-        8918 bp    DNA     circular SYN 18-JUN-2025
DEFINITION  Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 
            incorporated in the loop of the sgRNA constant region for 10X Direct
            Capture.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8918)
  AUTHORS   Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, 
            Guruvayurappan K, Norman TM
  TITLE     Comprehensive transcription factor perturbations recapitulate 
            fibroblast transcriptional states.
  JOURNAL   bioRxiv [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 
            10.1101/2024.07.31.606073.
  PUBMED    39131349
REFERENCE   2  (bases 1 to 8918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 18, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Aug 3:2024.07.31.606073. doi: 
            10.1101/2024.07.31.606073."
COMMENT     Sequence Label: pRCA893 - pBA904 Puro-T2A-BFP KLF5 g1 CRISPRa ...
FEATURES             Location/Qualifiers
     source          1..8918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(213..1004,1005..1073))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     836..855
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1074..1178)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1253..1272)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1444..1823
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1825..2023
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1978..1998
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2041..2221
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2268..2393
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2890..3123
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3308..3352
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3650..3767
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(4020..4053)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     4057..4077
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        4348..4559
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          4560..5498
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5455..5475
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5519..6115
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5519..5538)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6015..6035
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             6125..6178
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      6185..6194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6191..6901
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    complement(6924..6957)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7013..7601
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7066..7086)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7484..7495)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7604..7620)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7605..7621)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8130..8310
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(join(8372..8918,1..42))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8452..8471)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1201 cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct
     1261 gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg
     1321 agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga
     1381 agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc
     1441 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     1501 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     1561 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1621 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1681 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1741 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1801 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     1861 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     1921 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     1981 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact
     2041 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2101 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2161 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     2221 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     2281 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     2341 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     2401 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     2461 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     2521 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     2581 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     2641 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     2701 aaaacaaaag taagaccacc gcacagcaag cggccggccg ctgatcttca gacctggagg
     2761 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     2821 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     2881 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     2941 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     3001 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     3061 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     3121 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     3181 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     3241 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     3301 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     3361 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     3421 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     3481 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     3541 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     3601 ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa
     3661 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     3721 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     3781 gggacagcag agatccagtt tggttagtac cgggcccgct ctagagatcc gacgcgccat
     3841 ctctaggccc gcgccggccc cctcgcacgg acttgtggga gaagctcggc tactcccctg
     3901 ccccggttaa tttgcatata atatttccta gtaactatag aggcttaatg tgcgataaaa
     3961 gacagataat ctgttctttt taatactagc tacattttac atgataggct tggatttcta
     4021 taacttcgta tagcatacat tatacgaagt tataaacagc acaaaaggaa actcacccta
     4081 actgtaaagt aattgtgtgt tttgagacta taagtatccc ttggagaacc accttgttgg
     4141 aagttgtgta caaactgcgg tttaagagct aagctggaaa cagcatagca agtttaaata
     4201 aggctagtcc gttatcaact tggccgcttt aaggccggtc ctagcaaggc caagtggcac
     4261 cgagtcggtg ctttttttct cgagtactag gatccattag gcggccgcgt ggataaccgt
     4321 attaccgcca tgcatgtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     4381 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     4441 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     4501 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     4561 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     4621 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     4681 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     4741 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     4801 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     4861 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     4921 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     4981 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     5041 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     5101 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     5161 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     5221 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     5281 acctcgatta gttctcgcgc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     5341 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     5401 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     5461 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagct agcccaccat
     5521 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     5581 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     5641 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5701 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5761 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5821 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5881 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5941 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6001 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6061 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccggatc
     6121 gggagagggc agaggaagtc tgctaacatg cggtgacgtc gaggagaatc ctggcccacc
     6181 ggtcgccacc atggtgtcta agggcgaaga gctgattaag gagaacatgc acatgaagct
     6241 gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa
     6301 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt
     6361 cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac
     6421 ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt
     6481 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     6541 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat
     6601 gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg
     6661 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa
     6721 cgccaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta
     6781 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca
     6841 gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa
     6901 ttaagaattc gtcgagggac ctaataactt cgtatagcat acattatacg aagttataca
     6961 tgtttaaggg ttccggttcc actaggtaca attcgatatc aagcttatcg ataatcaacc
     7021 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     7081 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     7141 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     7201 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     7261 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     7321 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     7381 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     7441 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     7501 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     7561 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     7621 atcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg
     7681 attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg
     7741 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7801 aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga
     7861 tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca
     7921 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag
     7981 aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg
     8041 atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca
     8101 tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct
     8161 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8221 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8281 ccttttagtc agtgtggaaa atctctagca gcatgtgagc aaaaggccag caaaaggcca
     8341 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8401 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8461 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8521 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     8581 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     8641 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     8701 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     8761 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     8821 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     8881 ccggcaaaca aaccaccgct ggtagcggtg gttttttt
//
