LOCUS       sequence-476582-       11230 bp    DNA     circular SYN 01-JUL-2025
DEFINITION  pLV-U6-[empty]-EFSNC-dSaCas9-[empty linker]-P2A-Puro-WPRE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11230)
  TITLE     SNCA-targeted epigenome therapy for Parkinson's disease alleviates 
            pathological and behavioral perturbations in a mouse model
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 1, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBK1575
FEATURES             Location/Qualifiers
     source          1..11230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2634..2882
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2634..2654
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2805..2824
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2903..2978
                     /label=Sa gRNA scaffold
                     /note="guide RNA scaffold for the Staphylococcus aureus 
                     CRISPR/Cas9 system"
     promoter        3040..3251
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      3259..3268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3268..3333
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3343..3363
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3367..3387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6664..6684
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6703..6759
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6760..7356
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     7253..7273
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7372..7960
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7425..7445)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7843..7854)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8032..8265
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8291..8308)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8297..8521
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8567..8925
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8654..8673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8864..8885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8943..8962)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8997..9016
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9076..9092)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9076..9092)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9089..9111)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9100..9116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9124..9130,9131..9148,9149..9154))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9169..9190
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9307..9324)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9478..10066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9558..9577)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10237..11028,11029..11097))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10860..10879
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11098..11202)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta atgggcggga cgttaacggg gcggaacggt accgagggcc
     2641 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta
     2701 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     2761 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     2821 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     2881 ccggagacgt gtacacgtct ctgttttagt actctggaaa cagaatctac taaaacaagg
     2941 caaaatgccg tgtttatctc gtcaacttgt tggcgagatt ttttgaattc gctagctagg
     3001 tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     3061 cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc
     3121 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     3181 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     3241 ccagaacaca ggaccggtgc caccatggac tacaaagacc atgacggtga ttataaagat
     3301 catgacatag attacaagga tgacgatgac aagggtggat cgccaaagaa aaagaggaaa
     3361 gtcgccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc caagcggaac
     3421 tacatcctgg gcctggccat cggcatcacc agcgtgggct acggcatcat cgactacgag
     3481 acacgggacg tgatcgatgc cggcgtgcgg ctgttcaaag aggccaacgt ggaaaacaac
     3541 gagggcaggc ggagcaagag aggcgccaga aggctgaagc ggcggaggcg gcatagaatc
     3601 cagagagtga agaagctgct gttcgactac aacctgctga ccgaccacag cgagctgagc
     3661 ggcatcaacc cctacgaggc cagagtgaag ggcctgagcc agaagctgag cgaggaagag
     3721 ttctctgccg ccctgctgca cctggccaag agaagaggcg tgcacaacgt gaacgaggtg
     3781 gaagaggaca ccggcaacga gctgtccacc aaagagcaga tcagccggaa cagcaaggcc
     3841 ctggaagaga aatacgtggc cgaactgcag ctggaacggc tgaagaaaga cggcgaagtg
     3901 cggggcagca tcaacagatt caagaccagc gactacgtga aagaagccaa acagctgctg
     3961 aaggtgcaga aggcctacca ccagctggac cagagcttca tcgacaccta catcgacctg
     4021 ctggaaaccc ggcggaccta ctatgaggga cctggcgagg gcagcccctt cggctggaag
     4081 gacatcaaag aatggtacga gatgctgatg ggccactgca cctacttccc cgaggaactg
     4141 cggagcgtga agtacgccta caacgccgac ctgtacaacg ccctgaacga cctgaacaat
     4201 ctcgtgatca ccagggacga gaacgagaag ctggaatatt acgagaagtt ccagatcatc
     4261 gagaacgtgt tcaagcagaa gaagaagccc accctgaagc agatcgccaa agaaatcctc
     4321 gtgaacgaag aggatattaa gggctacaga gtgaccagca ccggcaagcc cgagttcacc
     4381 aacctgaagg tgtaccacga catcaaggac attaccgccc ggaaagagat tattgagaac
     4441 gccgagctgc tggatcagat tgccaagatc ctgaccatct accagagcag cgaggacatc
     4501 caggaagaac tgaccaatct gaactccgag ctgacccagg aagagatcga gcagatctct
     4561 aatctgaagg gctataccgg cacccacaac ctgagcctga aggccatcaa cctgatcctg
     4621 gacgagctgt ggcacaccaa cgacaaccag atcgctatct tcaaccggct gaagctggtg
     4681 cccaagaagg tggacctgtc ccagcagaaa gagatcccca ccaccctggt ggacgacttc
     4741 atcctgagcc ccgtcgtgaa gagaagcttc atccagagca tcaaagtgat caacgccatc
     4801 atcaagaagt acggcctgcc caacgacatc attatcgagc tggcccgcga gaagaactcc
     4861 aaggacgccc agaaaatgat caacgagatg cagaagcgga accggcagac caacgagcgg
     4921 atcgaggaaa tcatccggac caccggcaaa gagaacgcca agtacctgat cgagaagatc
     4981 aagctgcacg acatgcagga aggcaagtgc ctgtacagcc tggaagccat ccctctggaa
     5041 gatctgctga acaacccctt caactatgag gtggaccaca tcatccccag aagcgtgtcc
     5101 ttcgacaaca gcttcaacaa caaggtgctc gtgaagcagg aagaagccag caagaagggc
     5161 aaccggaccc cattccagta cctgagcagc agcgacagca agatcagcta cgaaaccttc
     5221 aagaagcaca tcctgaatct ggccaagggc aagggcagaa tcagcaagac caagaaagag
     5281 tatctgctgg aagaacggga catcaacagg ttctccgtgc agaaagactt catcaaccgg
     5341 aacctggtgg ataccagata cgccaccaga ggcctgatga acctgctgcg gagctacttc
     5401 agagtgaaca acctggacgt gaaagtgaag tccatcaatg gcggcttcac cagctttctg
     5461 cggcggaagt ggaagtttaa gaaagagcgg aacaaggggt acaagcacca cgccgaggac
     5521 gccctgatca ttgccaacgc cgatttcatc ttcaaagagt ggaagaaact ggacaaggcc
     5581 aaaaaagtga tggaaaacca gatgttcgag gaaaagcagg ccgagagcat gcccgagatc
     5641 gaaaccgagc aggagtacaa agagatcttc atcacccccc accagatcaa gcacattaag
     5701 gacttcaagg actacaagta cagccaccgg gtggacaaga agcctaatag agagctgatt
     5761 aacgacaccc tgtactccac ccggaaggac gacaagggca acaccctgat cgtgaacaat
     5821 ctgaacggcc tgtacgacaa ggacaatgac aagctgaaaa agctgatcaa caagagcccc
     5881 gaaaagctgc tgatgtacca ccacgacccc cagacctacc agaaactgaa gctgattatg
     5941 gaacagtacg gcgacgagaa gaatcccctg tacaagtact acgaggaaac cgggaactac
     6001 ctgaccaagt actccaaaaa ggacaacggc cccgtgatca agaagattaa gtattacggc
     6061 aacaaactga acgcccatct ggacatcacc gacgactacc ccaacagcag aaacaaggtc
     6121 gtgaagctgt ccctgaagcc ctacagattc gacgtgtacc tggacaatgg cgtgtacaag
     6181 ttcgtgaccg tgaagaatct ggatgtgatc aaaaaagaaa actactacga agtgaatagc
     6241 aagtgctatg aggaagctaa gaagctgaag aagatcagca accaggccga gtttatcgcc
     6301 tccttctaca acaacgatct gatcaagatc aacggcgagc tgtatagagt gatcggcgtg
     6361 aacaacgacc tgctgaaccg gatcgaagtg aacatgatcg acatcaccta ccgcgagtac
     6421 ctggaaaaca tgaacgacaa gaggcccccc aggatcatta agacaatcgc ctccaagacc
     6481 cagagcatta agaagtacag cacagacatt ctgggcaacc tgtatgaagt gaaatctaag
     6541 aagcaccctc agatcatcaa aaagggcaaa aggccggcgg ccacgaaaaa ggcgctcgag
     6601 ggtggaggaa gtggcgggtc agggtcgggt ggcagcgcta tacatcctag gggaggtggt
     6661 tcgccaaaga agaaacggaa ggtgggccgg cccggatccg gcgcaacaaa cttctctctg
     6721 ctgaaacaag ccggagatgt cgaagagaat cctggaccga ccgagtacaa gcccacggtg
     6781 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
     6841 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
     6901 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
     6961 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
     7021 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
     7081 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
     7141 ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
     7201 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
     7261 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
     7321 cgcacctggt gcatgacccg caagcccggt gcctgaacgc gttaagtcga caatcaacct
     7381 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     7441 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     7501 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     7561 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     7621 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     7681 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7741 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7801 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7861 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7921 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     7981 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     8041 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     8101 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8161 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8221 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc
     8281 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     8341 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     8401 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     8461 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     8521 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     8581 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     8641 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     8701 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     8761 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     8821 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     8881 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataatg gttacaaata
     8941 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     9001 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
     9061 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     9121 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     9181 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     9241 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     9301 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     9361 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     9421 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     9481 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     9541 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     9601 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     9661 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     9721 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     9781 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     9841 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     9901 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     9961 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
    10021 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
    10081 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
    10141 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
    10201 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
    10261 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
    10321 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
    10381 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
    10441 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
    10501 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
    10561 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
    10621 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
    10681 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
    10741 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
    10801 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
    10861 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
    10921 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
    10981 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
    11041 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
    11101 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
    11161 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
    11221 gccacctgac
//
